##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062962_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	781458
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.137162841765008	32.0	32.0	32.0	32.0	32.0
2	31.29833209206381	32.0	32.0	32.0	32.0	32.0
3	31.37825321386434	32.0	32.0	32.0	32.0	32.0
4	31.47828162230088	32.0	32.0	32.0	32.0	32.0
5	31.403677484906417	32.0	32.0	32.0	32.0	32.0
6	34.91071945005362	36.0	36.0	36.0	36.0	36.0
7	34.936860330305656	36.0	36.0	36.0	36.0	36.0
8	34.889672893488836	36.0	36.0	36.0	32.0	36.0
9	34.977027556183444	36.0	36.0	36.0	36.0	36.0
10	34.83794778478178	36.0	36.0	36.0	32.0	36.0
11	35.00596833099156	36.0	36.0	36.0	36.0	36.0
12	34.90546644861272	36.0	36.0	36.0	32.0	36.0
13	34.95545505964492	36.0	36.0	36.0	36.0	36.0
14	34.89859211883428	36.0	36.0	36.0	32.0	36.0
15	34.871719273460634	36.0	36.0	36.0	32.0	36.0
16	34.88490488292397	36.0	36.0	36.0	32.0	36.0
17	34.843985217375725	36.0	36.0	36.0	32.0	36.0
18	34.845243122471075	36.0	36.0	36.0	32.0	36.0
19	34.8248197599871	36.0	36.0	36.0	32.0	36.0
20	34.815635645165834	36.0	36.0	36.0	32.0	36.0
21	34.80370410181993	36.0	36.0	36.0	32.0	36.0
22	34.78711971724648	36.0	36.0	36.0	32.0	36.0
23	34.7265188916103	36.0	36.0	36.0	32.0	36.0
24	34.70903362688718	36.0	36.0	36.0	32.0	36.0
25	34.6783909052054	36.0	36.0	36.0	32.0	36.0
26	34.61872934949799	36.0	36.0	36.0	32.0	36.0
27	34.60105469519795	36.0	36.0	36.0	32.0	36.0
28	34.56748411302975	36.0	36.0	36.0	32.0	36.0
29	34.53569353695272	36.0	36.0	36.0	32.0	36.0
30	34.488474108653314	36.0	36.0	36.0	32.0	36.0
31	34.495293413081704	36.0	36.0	36.0	32.0	36.0
32	34.456268922961954	36.0	36.0	36.0	32.0	36.0
33	34.396337359141505	36.0	36.0	36.0	32.0	36.0
34	34.390617282054826	36.0	36.0	36.0	32.0	36.0
35	34.34465191987285	36.0	36.0	36.0	32.0	36.0
36	34.323587448077824	36.0	36.0	36.0	32.0	36.0
37	34.30447573637995	36.0	36.0	36.0	32.0	36.0
38	33.870946359241316	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	5.0
21	19.0
22	87.0
23	217.0
24	557.0
25	1334.0
26	2934.0
27	5670.0
28	9877.0
29	15124.0
30	22789.0
31	32215.0
32	45980.0
33	72580.0
34	172399.0
35	399669.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.26873117156686	18.478803375488546	12.15667303985933	26.095792413085267
2	15.508958894783852	21.276383375690056	36.77139398406568	26.44326374546041
3	17.959401476984656	25.373629003259296	29.023477939285208	27.643491580470837
4	12.038681640425054	16.3803208370017	35.82722507729162	35.753772445281626
5	13.859879686278322	37.334107954756306	33.10175735837033	15.70425500059504
6	33.69573284808652	35.94115102795032	16.799879200161747	13.563236923801409
7	29.23409439327203	31.231524824237894	21.169128317213808	18.365252465276267
8	28.212238671889494	32.67182039335023	19.73968604155656	19.376254893203708
9	27.75973402744186	13.868605665993543	18.656981907621116	39.714678398943484
10	15.922262759244637	27.078659881016513	31.49197712466393	25.50710023507492
11	37.184832546294516	21.37981341421277	22.048092550645627	19.387261488847084
12	24.47021969247149	24.343916995966502	28.884927622284557	22.300935689277452
13	30.03077592439744	19.418776513042975	25.223845263002985	25.326602299556594
14	23.81013926550021	19.870447126329406	25.551757806251658	30.767655801918725
15	25.118816366330627	26.885897898543494	23.238229053896692	24.75705668122919
16	25.276598358453047	25.998582137491717	24.195414212919953	24.52940529113529
17	23.73652838668233	25.758134154362743	25.826723893030717	24.67861356592421
18	24.74054907621395	24.441364731054005	27.164863626708026	23.65322256602402
19	25.16961884067986	25.003519063084646	26.358550299568247	23.468311796667255
20	25.629144496569232	23.85310534923182	26.202559830470733	24.31519032372821
21	26.95320797790796	24.12247363262005	25.02092242961234	23.903395959859647
22	25.45450357473284	24.376389231909112	25.675117120967627	24.49399007239042
23	23.917118920170914	24.064791792766588	26.287690813442072	25.73039847362043
24	24.28383867079229	25.205193369317353	26.117718418648217	24.39324954124214
25	24.735558404930273	24.162271036959122	26.061157477433206	25.0410130806774
26	24.134630396003367	25.297072907309158	26.480245899331763	24.088050797355713
27	25.115489252141508	24.614630600748857	25.651026670659206	24.618853476450433
28	24.133990566351613	24.651228856829157	26.578907631632156	24.635872945187074
29	24.10622195946551	24.832684546066456	26.682688001146577	24.37840549332146
30	24.08037284153467	25.029880044736892	26.77674295995434	24.1130041537741
31	24.41717917021772	24.93710474523263	25.64654786309693	24.99916822145272
32	24.129255826928638	25.097318090031713	25.62479365493731	25.148632428102342
33	24.050684745693307	24.479626544228864	26.262959749596266	25.206728960481563
34	24.603241632947643	24.744771951915524	26.423812924047102	24.228173491089734
35	25.31562796721001	24.683860169068588	26.21228524117739	23.788226622544016
36	24.24391330052287	25.41121851718199	25.88661194843485	24.458256233860297
37	25.103332488758195	25.206856926411913	25.81904593720968	23.87076464762022
38	24.27564885124984	24.931090346506146	26.094300653394043	24.69896014884997
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	404.0
1	310.0
2	216.0
3	216.0
4	778.0
5	1340.0
6	1340.0
7	1493.5
8	1647.0
9	1519.0
10	1391.0
11	1391.0
12	1721.5
13	2052.0
14	2542.5
15	3033.0
16	3033.0
17	4529.5
18	6026.0
19	6026.0
20	6821.5
21	7617.0
22	7397.0
23	7177.0
24	7177.0
25	7769.0
26	8361.0
27	8361.0
28	10634.0
29	12907.0
30	15485.0
31	18063.0
32	18063.0
33	22477.0
34	26891.0
35	26891.0
36	29430.0
37	31969.0
38	36398.5
39	40828.0
40	40828.0
41	44144.5
42	47461.0
43	53889.5
44	60318.0
45	60318.0
46	64307.0
47	68296.0
48	68296.0
49	71415.0
50	74534.0
51	72973.5
52	71413.0
53	71413.0
54	68454.5
55	65496.0
56	65496.0
57	63308.5
58	61121.0
59	54687.0
60	48253.0
61	48253.0
62	45412.0
63	42571.0
64	35113.5
65	27656.0
66	27656.0
67	22900.5
68	18145.0
69	18145.0
70	14550.5
71	10956.0
72	8486.0
73	6016.0
74	6016.0
75	4452.5
76	2889.0
77	2889.0
78	2832.5
79	2776.0
80	2107.0
81	1438.0
82	1438.0
83	1473.0
84	1508.0
85	1508.0
86	935.0
87	362.0
88	301.0
89	240.0
90	240.0
91	142.5
92	45.0
93	35.0
94	25.0
95	25.0
96	16.5
97	8.0
98	8.0
99	8.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007677955821042207
2	0.0
3	1.2796593035070342E-4
4	2.5593186070140683E-4
5	1.2796593035070342E-4
6	0.0
7	5.118637214028137E-4
8	0.002175420815961958
9	0.0030711823284168822
10	6.398296517535172E-4
11	0.0061423646568337644
12	2.5593186070140683E-4
13	3.838977910521103E-4
14	1.2796593035070342E-4
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	1.2796593035070342E-4
23	1.2796593035070342E-4
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	781458.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.693631060810404
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.51124473399317	49.25395801519223
2	11.590420020584407	13.837485130971919
3	2.861384628482335	5.1241931500709565
4	1.0503561954870713	2.507983008633667
5	0.5230297575486134	1.561077269046602
6	0.292428743422784	1.0473680112873631
7	0.19031971476436976	0.7952612385720071
8	0.14338480531953587	0.6847327734776398
9	0.09786136025060177	0.5257529940517629
>10	0.5685208014739174	6.589706289407394
>50	0.07884683937642531	3.309567040553505
>100	0.08380077880187162	10.200982583576147
>500	0.007109063495749496	2.902404972959392
>1k	0.0012925569992271813	1.6595275221994221
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4149	0.5309306450250686	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3171	0.4057799651420806	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1822	0.23315392509898164	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1385	0.17723281353572423	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1213	0.15522267351540325	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1165	0.1490803088585695	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	966	0.1236150887187795	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	865	0.11069052975335847	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	851	0.10889900672844861	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	831	0.10633968812143454	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	830	0.10621172219108385	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	828	0.10595579033038244	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	814	0.10416426730547258	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	805	0.10301257393231626	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	803	0.10275664207161485	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	802	0.10262867614126414	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2796593035070342E-4
2	0.0	0.0	0.0	0.0	1.2796593035070342E-4
3	0.0	0.0	0.0	0.0	1.2796593035070342E-4
4	0.0	0.0	0.0	0.0	1.2796593035070342E-4
5	0.0	0.0	0.0	0.0	2.5593186070140683E-4
6	0.0	0.0	0.0	0.0	2.5593186070140683E-4
7	0.0	0.0	0.0	0.0	2.5593186070140683E-4
8	0.0	0.0	0.0	0.0	2.5593186070140683E-4
9	0.0	0.0	0.0	0.0	2.5593186070140683E-4
10	0.0	0.0	0.0	0.0	2.5593186070140683E-4
11	0.0	0.0	0.0	0.0	2.5593186070140683E-4
12	0.0	0.0	0.0	0.0	3.838977910521103E-4
13	0.0	0.0	0.0	0.0	6.398296517535171E-4
14	0.0	0.0	0.0	0.0	6.398296517535171E-4
15	0.0	0.0	0.0	0.0	6.398296517535171E-4
16	0.0	0.0	0.0	0.0	6.398296517535171E-4
17	0.0	0.0	0.0	0.0	7.677955821042206E-4
18	0.0	0.0	0.0	0.0	0.0010237274428056273
19	0.0	0.0	0.0	1.2796593035070342E-4	0.0010237274428056273
20	0.0	0.0	0.0	2.5593186070140683E-4	0.0012796593035070342
21	0.0	0.0	0.0	3.838977910521103E-4	0.0014076252338577377
22	0.0	0.0	0.0	7.677955821042206E-4	0.0014076252338577377
23	0.0	0.0	0.0	0.0020474548856112547	0.0014076252338577377
24	0.0	0.0	0.0	0.0037110119801703994	0.0014076252338577377
25	0.0	0.0	0.0	0.005118637214028137	0.0014076252338577377
26	0.0	0.0	0.0	0.007422023960340799	0.0014076252338577377
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATCCG	20	0.0037535585	31.998657	27
TACCGGA	35	0.0020656877	22.857645	2
GTTTACG	45	3.5363718E-4	21.332438	30
TATACTG	95	1.718945E-9	20.212263	5
AGACACG	40	0.0044799875	20.00172	5
CGACAAT	50	7.195152E-4	19.199194	19
ATATCGA	50	7.195152E-4	19.199194	15
TATCGAC	60	1.1379567E-4	18.665882	16
CCGAGTT	45	0.008849949	17.77703	31
TATCCGA	45	0.008849949	17.77703	28
TCGACAA	55	0.0013644997	17.453814	18
TCGTTTA	195	0.0	17.230045	30
CGGCATC	195	0.0	17.230045	25
TCTAGCG	155	1.0913936E-11	16.515434	28
ATCGTTT	205	0.0	16.389557	29
TACCGTC	170	3.6379788E-12	16.001377	7
ACGATCA	120	3.1021045E-8	16.001375	9
CGATCAG	140	7.403287E-10	16.000353	10
GATTACT	60	0.002440609	16.00035	2
CATCGTT	210	0.0	15.999329	28
>>END_MODULE