FastQCFastQC Report
Thu 2 Feb 2017
SRR4062961_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062961_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1303697
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT37730.28940773814774445No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT26820.2057226487443018No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC18390.14106038442981766No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT18280.1402166300911945No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA16390.12571939645485108No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG16170.12403188777760477No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG15970.1224977889801081No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC15290.11728185306861948No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG15150.1162079839103718No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT14970.11482729499262483No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT14550.11160568751788183No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC14030.10761703064439054No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA13740.10539258738802038No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG13530.10378178365064887No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG13060.10017665147653175No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC13050.10009994653665691No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG4050.018.17175128
TAGCGGC4150.017.73319830
CTAGCGG4100.017.55992129
GTCCTAC8750.016.2939361
ATCGTTT3150.016.25299329
GATATAC3450.016.2514821
TCCTACA9050.016.0985492
GCTATAC805.622824E-515.9996553
CGCTTCG3100.015.99842832
GGACCGT908.596839E-615.99726
CGCAAGA4850.015.8450432
ACGTTTT4150.015.80628229
GTAGGAC7550.015.681783
CGGTCCA5000.015.67966210
AAGACGG5500.015.4160755
CCTACAG9150.015.3876463
CGCCGGT4800.015.3318267
AGCGGCG4700.015.31764331
CACGTTT4050.015.01144628
TAGGACG6400.014.9996774
Produced by FastQC (version 0.11.5)