##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062961_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1303697
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28212920640302	32.0	32.0	32.0	32.0	32.0
2	30.986434731383135	32.0	32.0	32.0	32.0	32.0
3	31.036626608790232	32.0	32.0	32.0	32.0	32.0
4	31.082225394397625	32.0	32.0	32.0	32.0	32.0
5	31.04153112264583	32.0	32.0	32.0	32.0	32.0
6	34.65947455582087	36.0	36.0	36.0	32.0	36.0
7	34.599470582504985	36.0	36.0	36.0	32.0	36.0
8	34.587933392498414	36.0	36.0	36.0	32.0	36.0
9	34.689917979407795	36.0	36.0	36.0	32.0	36.0
10	34.46905761077919	36.0	36.0	36.0	32.0	36.0
11	34.67983051276485	36.0	36.0	36.0	32.0	36.0
12	34.53173014895332	36.0	36.0	36.0	32.0	36.0
13	34.60176712840484	36.0	36.0	36.0	32.0	36.0
14	34.51093927500025	36.0	36.0	36.0	32.0	36.0
15	34.47925860073315	36.0	36.0	36.0	32.0	36.0
16	34.49685471394043	36.0	36.0	36.0	32.0	36.0
17	34.41796291622977	36.0	36.0	36.0	32.0	36.0
18	34.4446010077495	36.0	36.0	36.0	32.0	36.0
19	34.42945408327242	36.0	36.0	36.0	32.0	36.0
20	34.396009195388196	36.0	36.0	36.0	32.0	36.0
21	34.368755163201264	36.0	36.0	36.0	32.0	36.0
22	34.34976762238465	36.0	36.0	36.0	32.0	36.0
23	34.31227501482323	36.0	36.0	36.0	32.0	36.0
24	34.30013569103864	36.0	36.0	36.0	32.0	36.0
25	34.27116730344551	36.0	36.0	36.0	32.0	36.0
26	34.238704238791684	36.0	36.0	36.0	32.0	36.0
27	34.23595513374657	36.0	36.0	36.0	32.0	36.0
28	34.21960240761465	36.0	36.0	36.0	32.0	36.0
29	34.172336056614384	36.0	36.0	36.0	32.0	36.0
30	34.15170166073865	36.0	36.0	36.0	32.0	36.0
31	34.15690915910675	36.0	36.0	36.0	32.0	36.0
32	34.119948116778666	36.0	36.0	36.0	32.0	36.0
33	34.07579828748551	36.0	36.0	36.0	32.0	36.0
34	34.081692295065494	36.0	36.0	36.0	32.0	36.0
35	34.03782320585228	36.0	36.0	36.0	32.0	36.0
36	34.011591650513886	36.0	36.0	36.0	32.0	36.0
37	33.99041495071324	36.0	36.0	36.0	32.0	36.0
38	33.351770388364784	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	0.0
9	4.0
10	10.0
11	9.0
12	7.0
13	8.0
14	140.0
15	288.0
16	444.0
17	483.0
18	655.0
19	909.0
20	1195.0
21	1597.0
22	2279.0
23	3385.0
24	4843.0
25	7243.0
26	10602.0
27	15506.0
28	22039.0
29	30613.0
30	41549.0
31	56958.0
32	79785.0
33	122570.0
34	283511.0
35	617063.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.2067355913303	17.51634669587082	11.558346606783822	25.718571106015066
2	16.277847109334587	19.506830053771612	38.616397962112735	25.59892487478107
3	19.05526851777269	23.670299069077018	28.534342888047593	28.740089525102704
4	12.21152415273284	15.319231518599146	36.412122160498654	36.05712216816936
5	14.375766092349892	36.871228984715735	33.636957752356	15.116047170578373
6	34.044467558288304	35.931032789953456	16.84515974632045	13.179339905437788
7	30.342579865244655	30.3989580392975	20.669772707747818	18.58868938771002
8	27.890176514084615	33.771473630274635	19.32810817278928	19.01024168285147
9	27.599323461392427	13.95372383878131	18.813986400193297	39.63296629963297
10	16.06573321653701	26.6415278431902	31.62068706306848	25.672051877204318
11	37.81313426516138	20.782112706090633	22.481387217970138	18.92336581077785
12	25.006462539897072	23.76043882371802	28.998969828951722	22.234128807433187
13	29.950656077771914	19.35874381666646	25.213808319728976	25.47679178583265
14	23.130143543606202	20.435018748427403	25.167922698791635	31.266915009174763
15	24.78335756455158	28.403923539317873	22.251086568744412	24.561632327386135
16	25.173966309106543	25.800406119563625	24.915136046985285	24.110491524344543
17	23.508454018257517	26.30377693881606	25.672995704519426	24.514773338406997
18	24.602380729560597	24.662366891320723	27.499129356859363	23.236123022259314
19	25.577743582033225	24.793380822119566	25.47809690964908	24.15077868619813
20	25.642070057953337	24.457382626271922	25.57771155269688	24.322835763077855
21	26.888082411087353	24.21803743601427	24.408891916376895	24.484988236521477
22	25.620333641962763	24.975663789790374	25.36281856655311	24.041184001693754
23	23.88024894693116	24.66325194408283	26.13275379706974	25.32374531191627
24	24.67583777389426	25.28189187074324	25.880657975288337	24.161612380074168
25	24.613171613022015	24.933313386805374	25.860443349942653	24.593071650229962
26	23.661982204259747	26.19110090236893	26.596023807441437	23.55089308592989
27	24.754272283110243	25.207103224435535	25.525458028179386	24.51316646427484
28	23.63609166746572	25.46767667082175	26.16066736983412	24.735564291878415
29	23.92398013430974	25.205476719798337	25.871962342948585	24.998580802943337
30	23.62557031391428	25.59478333440723	26.48511737650997	24.294528975168525
31	24.937136492438736	25.210989722449366	24.973113289322484	24.87876049578941
32	24.19498978611049	25.596781876885622	25.0179308800054	25.19029745699849
33	23.66451389203192	25.171430565922236	26.182408719312605	24.98164682273324
34	24.680502419965464	25.431999699278347	26.121434779840296	23.766063100915897
35	25.12299813958841	25.233318629049272	26.09248355357793	23.551199677784385
36	23.609985945660647	25.95212011857198	25.80774093372366	24.63015300204371
37	24.857737266929227	25.724354735519427	25.07337519772564	24.34453279982571
38	23.916917315438507	25.861736997906593	25.566098018052845	24.65524766860205
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	70.0
1	102.0
2	134.0
3	134.0
4	408.5
5	683.0
6	683.0
7	905.0
8	1127.0
9	1220.5
10	1314.0
11	1314.0
12	1650.0
13	1986.0
14	2645.5
15	3305.0
16	3305.0
17	4852.0
18	6399.0
19	6399.0
20	7658.0
21	8917.0
22	9826.5
23	10736.0
24	10736.0
25	13314.5
26	15893.0
27	15893.0
28	20799.5
29	25706.0
30	32229.5
31	38753.0
32	38753.0
33	47645.0
34	56537.0
35	56537.0
36	61458.0
37	66379.0
38	73249.5
39	80120.0
40	80120.0
41	81332.5
42	82545.0
43	90645.0
44	98745.0
45	98745.0
46	99012.5
47	99280.0
48	99280.0
49	105442.0
50	111604.0
51	112465.5
52	113327.0
53	113327.0
54	108796.0
55	104265.0
56	104265.0
57	100704.0
58	97143.0
59	86902.5
60	76662.0
61	76662.0
62	73503.5
63	70345.0
64	58841.5
65	47338.0
66	47338.0
67	40047.0
68	32756.0
69	32756.0
70	26282.5
71	19809.0
72	15517.0
73	11225.0
74	11225.0
75	8509.0
76	5793.0
77	5793.0
78	5619.0
79	5445.0
80	4285.5
81	3126.0
82	3126.0
83	3156.5
84	3187.0
85	3187.0
86	2124.5
87	1062.0
88	902.0
89	742.0
90	742.0
91	522.5
92	303.0
93	261.0
94	219.0
95	219.0
96	204.0
97	189.0
98	189.0
99	358.5
100	528.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12265119885985779
2	0.059753148162494815
3	0.011659150860974597
4	0.002684672895619151
5	2.3011481962449863E-4
6	3.835246993741644E-4
7	7.670493987483289E-5
8	3.835246993741644E-4
9	1.5340987974966577E-4
10	2.3011481962449863E-4
11	0.0017642136171211562
12	0.0023011481962449862
13	0.014804053395842747
14	0.009281297724854778
15	0.021630793044702872
16	0.010738691582476603
17	0.017948955930710893
18	0.004832411212114471
19	0.006980149528609792
20	0.004755706272239639
21	0.006596624829235628
22	0.006443214949485961
23	0.009664822424228942
24	0.014037003997094415
25	0.01626144725346457
26	0.01833248063008506
27	0.009204592784979945
28	0.005522755670987967
29	0.010968806402101102
30	0.0022244432563701534
31	0.006059690250111797
32	0.006136395189986631
33	0.008284133506481951
34	0.012733020019222257
35	0.0170284966522129
36	0.014190413876844082
37	0.009434707604604445
38	0.006136395189986631
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1303697.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.02840710307727
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.63071349588125	42.48292199560905
2	13.368752196080639	14.445847722200062
3	3.9059835497042483	6.331022080840319
4	1.6063867250795991	3.471620637903186
5	0.7236256622211943	1.9548170934360283
6	0.40225206178949685	1.3039822891449078
7	0.23934992056486815	0.9052186463857532
8	0.18658471870878984	0.8064700113289325
9	0.13241401161061664	0.6438706312904996
>10	0.6155532064108521	6.299313082416023
>50	0.08094424152738691	3.041630343925557
>100	0.09180738995050727	10.651309505117142
>500	0.011085090879113422	4.124876091763339
>1k	0.0045477295914311475	3.537099868639265
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3773	0.28940773814774445	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2682	0.2057226487443018	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1839	0.14106038442981766	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1828	0.1402166300911945	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1639	0.12571939645485108	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1617	0.12403188777760477	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1597	0.1224977889801081	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1529	0.11728185306861948	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1515	0.1162079839103718	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1497	0.11482729499262483	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1455	0.11160568751788183	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1403	0.10761703064439054	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1374	0.10539258738802038	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1353	0.10378178365064887	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1306	0.10017665147653175	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1305	0.10009994653665691	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.670493987483289E-5
2	0.0	0.0	0.0	0.0	7.670493987483289E-5
3	0.0	0.0	0.0	0.0	7.670493987483289E-5
4	0.0	0.0	0.0	0.0	7.670493987483289E-5
5	0.0	0.0	0.0	0.0	7.670493987483289E-5
6	0.0	0.0	0.0	0.0	7.670493987483289E-5
7	0.0	0.0	0.0	7.670493987483289E-5	7.670493987483289E-5
8	0.0	0.0	0.0	1.5340987974966577E-4	7.670493987483289E-5
9	0.0	0.0	0.0	1.5340987974966577E-4	7.670493987483289E-5
10	0.0	0.0	0.0	1.5340987974966577E-4	7.670493987483289E-5
11	0.0	0.0	0.0	1.5340987974966577E-4	7.670493987483289E-5
12	0.0	0.0	0.0	3.0681975949933154E-4	3.0681975949933154E-4
13	0.0	0.0	0.0	3.0681975949933154E-4	4.6022963924899726E-4
14	0.0	0.0	0.0	3.0681975949933154E-4	4.6022963924899726E-4
15	0.0	0.0	0.0	3.8352469937416437E-4	6.903444588734959E-4
16	0.0	0.0	0.0	4.6022963924899726E-4	6.903444588734959E-4
17	0.0	0.0	0.0	5.369345791238301E-4	6.903444588734959E-4
18	0.0	0.0	0.0	5.369345791238301E-4	9.204592784979945E-4
19	0.0	0.0	0.0	6.136395189986631E-4	9.204592784979945E-4
20	0.0	0.0	0.0	6.136395189986631E-4	0.0010738691582476603
21	0.0	0.0	0.0	7.670493987483287E-4	0.0010738691582476603
22	0.0	0.0	0.0	0.0011505740981224931	0.0010738691582476603
23	0.0	0.0	0.0	0.0013806889177469918	0.0010738691582476603
24	0.0	0.0	0.0	0.0020710333766204877	0.0010738691582476603
25	0.0	0.0	0.0	0.002377853136119819	0.0010738691582476603
26	0.0	0.0	0.0	0.002991492655118482	0.0012272790379973262
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	405	0.0	18.171751	28
TAGCGGC	415	0.0	17.733198	30
CTAGCGG	410	0.0	17.559921	29
GTCCTAC	875	0.0	16.293936	1
ATCGTTT	315	0.0	16.252993	29
GATATAC	345	0.0	16.251482	1
TCCTACA	905	0.0	16.098549	2
GCTATAC	80	5.622824E-5	15.999655	3
CGCTTCG	310	0.0	15.998428	32
GGACCGT	90	8.596839E-6	15.9972	6
CGCAAGA	485	0.0	15.845043	2
ACGTTTT	415	0.0	15.806282	29
GTAGGAC	755	0.0	15.68178	3
CGGTCCA	500	0.0	15.679662	10
AAGACGG	550	0.0	15.416075	5
CCTACAG	915	0.0	15.387646	3
CGCCGGT	480	0.0	15.331826	7
AGCGGCG	470	0.0	15.317643	31
CACGTTT	405	0.0	15.011446	28
TAGGACG	640	0.0	14.999677	4
>>END_MODULE