FastQCFastQC Report
Thu 2 Feb 2017
SRR4062961_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062961_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1303697
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT42170.32346473145217025No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT31460.24131374084622426No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC18600.14267118816718916No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT18550.142287663467815No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT18110.13891264611332235No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG17480.13408023490120788No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC16600.12733020019222258No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC16220.12441541247697893No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA16060.12318813343898159No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG15320.11751196788824397No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG15020.11521081969199899No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA14750.1131397863153785No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT14450.11083863811913351No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC14380.11030170354000969No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG14270.10945794920138652No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA13500.10355166883102439No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG13250.10163404533415357No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13220.10140393051452908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAATA659.437072E-619.6919062
ATACCGT3950.018.633966
TCTAGCG4050.018.56752428
TAGCGGC4100.018.3410930
CTAGCGG4100.018.3410929
TGCGTAC1055.980837E-918.28534116
TATTCGG450.00884802317.7787785
CCGTGCG1006.817754E-817.5996413
TCACGTT3150.017.2694927
ACCGTCG4200.017.1438228
CGTCGTA4050.016.9879610
TACCGTC4300.016.7451297
CATCGTT3000.016.53299528
CCGTCGT4200.016.3818749
ATCGTTT3050.016.26196329
TGTAGGA9550.016.2509782
GTATTAG3500.016.0015161
GACTGCG703.6952866E-416.0009027
GTTATTC2200.016.0002883
TCTATAC805.6209246E-516.0002883
Produced by FastQC (version 0.11.5)