##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062961_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1303697
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.172209493463587	32.0	32.0	32.0	32.0	32.0
2	31.26140583279704	32.0	32.0	32.0	32.0	32.0
3	31.352039622703742	32.0	32.0	32.0	32.0	32.0
4	31.457903945471994	32.0	32.0	32.0	32.0	32.0
5	31.364147497462984	32.0	32.0	32.0	32.0	32.0
6	34.88896576428419	36.0	36.0	36.0	36.0	36.0
7	34.92768104858721	36.0	36.0	36.0	36.0	36.0
8	34.84785805290647	36.0	36.0	36.0	32.0	36.0
9	34.978088466875356	36.0	36.0	36.0	36.0	36.0
10	34.816949030334506	36.0	36.0	36.0	32.0	36.0
11	34.9904379621952	36.0	36.0	36.0	36.0	36.0
12	34.87910150901628	36.0	36.0	36.0	32.0	36.0
13	34.951718842645185	36.0	36.0	36.0	36.0	36.0
14	34.88903403168067	36.0	36.0	36.0	32.0	36.0
15	34.86072990886686	36.0	36.0	36.0	32.0	36.0
16	34.875936663197045	36.0	36.0	36.0	32.0	36.0
17	34.84307473285587	36.0	36.0	36.0	32.0	36.0
18	34.83661387577021	36.0	36.0	36.0	32.0	36.0
19	34.821817492868355	36.0	36.0	36.0	32.0	36.0
20	34.810449820778906	36.0	36.0	36.0	32.0	36.0
21	34.79695435365733	36.0	36.0	36.0	32.0	36.0
22	34.777370815457886	36.0	36.0	36.0	32.0	36.0
23	34.725330348999805	36.0	36.0	36.0	32.0	36.0
24	34.70960967157246	36.0	36.0	36.0	32.0	36.0
25	34.68350467938486	36.0	36.0	36.0	32.0	36.0
26	34.61810681469697	36.0	36.0	36.0	32.0	36.0
27	34.60427614698814	36.0	36.0	36.0	32.0	36.0
28	34.562031668401474	36.0	36.0	36.0	32.0	36.0
29	34.530944690369004	36.0	36.0	36.0	32.0	36.0
30	34.49765014416693	36.0	36.0	36.0	32.0	36.0
31	34.498489296209165	36.0	36.0	36.0	32.0	36.0
32	34.46826064645389	36.0	36.0	36.0	32.0	36.0
33	34.43874305149126	36.0	36.0	36.0	32.0	36.0
34	34.423665161460065	36.0	36.0	36.0	32.0	36.0
35	34.387199633043565	36.0	36.0	36.0	32.0	36.0
36	34.3564800716731	36.0	36.0	36.0	32.0	36.0
37	34.341049338918474	36.0	36.0	36.0	32.0	36.0
38	33.89454067931429	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	5.0
21	18.0
22	92.0
23	271.0
24	816.0
25	2141.0
26	4450.0
27	8751.0
28	15406.0
29	24636.0
30	37132.0
31	54400.0
32	78489.0
33	125600.0
34	303508.0
35	647982.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.5039199501683	18.783235193941643	12.234540360911577	26.478304494978477
2	15.363232407530278	20.570117136113684	37.62852871487777	26.43812174147827
3	18.110266419267667	24.370233267392656	28.877415534437834	28.642084778901843
4	11.773878932968012	16.222939331482692	36.39604783648886	35.607133899060436
5	13.837187628720477	37.38215244799981	33.3090434357063	15.471616487573417
6	34.20858850529571	36.34313520943533	16.359795535155435	13.088480750113524
7	29.94181921384986	30.713165272552246	20.73805606372656	18.606959449871326
8	27.770554551974623	33.94046783491157	19.1240847917218	19.164892821391998
9	27.404727185420025	13.751916732315628	18.633744767579667	40.20961131468468
10	15.854955982532617	26.901549221553942	31.525665286222843	25.717829509690592
11	37.9231731629991	20.84029784456208	22.32507155004514	18.91145744239368
12	24.803232049263134	23.882616536255085	28.94147625246128	22.372675162020506
13	29.79490617444558	19.365480909659922	25.285342595206245	25.55427032068826
14	23.04876056322903	20.390704281746448	25.05421121625654	31.506323938767984
15	24.71218388935466	28.455308250306626	22.132826876183653	24.699680984155062
16	25.14602702928671	25.85209600083455	24.758820492798556	24.24305647708018
17	23.541589801924832	26.436127413041525	25.476625320147246	24.545657464886396
18	24.597279889422158	24.65266085601179	27.388419241587574	23.361640012978476
19	25.505006147900932	24.76395972377017	25.614617506982068	24.116416621346833
20	25.803158249194407	24.40175899768121	25.4846793388341	24.31040341429028
21	26.880060136765117	24.106328550772997	24.52283701325847	24.49077429920342
22	25.53722222264836	24.961474943947863	25.406517005101648	24.09478582830213
23	23.793295210919734	24.676247128354408	26.149674578793352	25.380783081932506
24	24.8080650641982	25.161444722201555	25.85217270577442	24.178317507825824
25	24.66646774518926	24.794028060201104	25.889911536192844	24.649592658416793
26	23.860759056744012	26.081827295759673	26.49097144505203	23.56644220244428
27	24.87901676238194	25.027134455734597	25.56322692535743	24.53062185652604
28	23.685871793829392	25.28486297046016	26.26799018483589	24.761275050874552
29	23.94306345722971	24.977199456622206	26.011412160954578	25.068324925193508
30	23.71103101410834	25.43075576610209	26.474633292858694	24.383579926930874
31	24.932633886554928	24.950199317786264	25.09823985174469	25.018926943914117
32	24.274735617248485	25.482685010397354	25.16566349389467	25.076915878459488
33	23.780679099514686	24.970296012033472	26.203864855100534	25.04516003335131
34	24.657569972163778	25.191359648752737	26.32076318346978	23.830307195613702
35	25.24213831894988	25.111433101403165	25.999292780454354	23.647135799192604
36	23.675286512126668	25.78589963772257	25.877178516173622	24.661635333977145
37	25.08734775028247	25.464122414947642	25.04377934443356	24.404750490336326
38	24.081994575422492	25.37324652372946	25.780473361888046	24.764285538960003
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	33.0
1	49.5
2	66.0
3	66.0
4	303.5
5	541.0
6	541.0
7	801.0
8	1061.0
9	1216.5
10	1372.0
11	1372.0
12	1740.0
13	2108.0
14	2723.0
15	3338.0
16	3338.0
17	5094.5
18	6851.0
19	6851.0
20	8008.5
21	9166.0
22	10046.0
23	10926.0
24	10926.0
25	13397.5
26	15869.0
27	15869.0
28	20752.0
29	25635.0
30	31853.5
31	38072.0
32	38072.0
33	47412.5
34	56753.0
35	56753.0
36	61639.0
37	66525.0
38	73241.5
39	79958.0
40	79958.0
41	80931.5
42	81905.0
43	90130.0
44	98355.0
45	98355.0
46	98684.5
47	99014.0
48	99014.0
49	105513.5
50	112013.0
51	112483.5
52	112954.0
53	112954.0
54	108312.0
55	103670.0
56	103670.0
57	101075.0
58	98480.0
59	87764.0
60	77048.0
61	77048.0
62	74113.5
63	71179.0
64	59369.5
65	47560.0
66	47560.0
67	40392.5
68	33225.0
69	33225.0
70	26863.5
71	20502.0
72	15884.0
73	11266.0
74	11266.0
75	8478.5
76	5691.0
77	5691.0
78	5480.5
79	5270.0
80	4206.0
81	3142.0
82	3142.0
83	2871.0
84	2600.0
85	2600.0
86	1716.5
87	833.0
88	688.0
89	543.0
90	543.0
91	324.0
92	105.0
93	71.0
94	37.0
95	37.0
96	26.0
97	15.0
98	15.0
99	15.5
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008360838446356784
2	0.0
3	0.0
4	4.6022963924899726E-4
5	0.0
6	7.670493987483289E-5
7	1.5340987974966577E-4
8	0.0024545580759946523
9	0.0035284272342423124
10	7.670493987483289E-4
11	0.004909116151989305
12	3.0681975949933154E-4
13	7.670493987483289E-5
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.5340987974966577E-4
22	0.0
23	1.5340987974966577E-4
24	0.0
25	0.0
26	0.0
27	3.0681975949933154E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	7.670493987483289E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1303697.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.27560809340604
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.19878170302992	40.35613257595998
2	14.192145159720514	14.838060367485584
3	4.218449888243569	6.6156609935848
4	1.7146123913987275	3.5852962161943043
5	0.7922540930312975	2.070778223885048
6	0.4325638862851101	1.3567524116880647
7	0.26827725642150413	0.9817049701945326
8	0.196355531972851	0.8211683869108015
9	0.12093744633840077	0.5689870693743206
>10	0.6691581369084654	6.552455269719879
>50	0.0811608019640858	2.9750365595742334
>100	0.0970202665658005	10.726838390298397
>500	0.013417684426631101	4.759927829556877
>1k	0.004865753693173916	3.7912007355731534
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4217	0.32346473145217025	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3146	0.24131374084622426	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1860	0.14267118816718916	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1855	0.142287663467815	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1811	0.13891264611332235	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1748	0.13408023490120788	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1660	0.12733020019222258	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1622	0.12441541247697893	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1606	0.12318813343898159	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1532	0.11751196788824397	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1502	0.11521081969199899	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1475	0.1131397863153785	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1445	0.11083863811913351	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1438	0.11030170354000969	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1427	0.10945794920138652	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1350	0.10355166883102439	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1325	0.10163404533415357	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1322	0.10140393051452908	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.670493987483289E-5
2	0.0	0.0	0.0	0.0	7.670493987483289E-5
3	0.0	0.0	0.0	0.0	7.670493987483289E-5
4	0.0	0.0	0.0	0.0	7.670493987483289E-5
5	0.0	0.0	0.0	0.0	7.670493987483289E-5
6	0.0	0.0	0.0	0.0	7.670493987483289E-5
7	0.0	0.0	0.0	0.0	7.670493987483289E-5
8	0.0	0.0	0.0	7.670493987483289E-5	7.670493987483289E-5
9	0.0	0.0	0.0	7.670493987483289E-5	7.670493987483289E-5
10	0.0	0.0	0.0	1.5340987974966577E-4	7.670493987483289E-5
11	0.0	0.0	0.0	1.5340987974966577E-4	7.670493987483289E-5
12	0.0	0.0	0.0	1.5340987974966577E-4	4.6022963924899726E-4
13	0.0	0.0	0.0	1.5340987974966577E-4	4.6022963924899726E-4
14	0.0	0.0	0.0	1.5340987974966577E-4	4.6022963924899726E-4
15	0.0	0.0	0.0	1.5340987974966577E-4	6.136395189986631E-4
16	0.0	0.0	0.0	2.3011481962449863E-4	6.136395189986631E-4
17	0.0	0.0	0.0	3.0681975949933154E-4	6.136395189986631E-4
18	0.0	0.0	0.0	3.0681975949933154E-4	6.136395189986631E-4
19	0.0	0.0	0.0	3.8352469937416437E-4	6.136395189986631E-4
20	0.0	0.0	0.0	3.8352469937416437E-4	7.670493987483287E-4
21	0.0	0.0	0.0	5.369345791238301E-4	8.437543386231617E-4
22	0.0	0.0	0.0	9.204592784979945E-4	9.204592784979945E-4
23	0.0	0.0	0.0	0.0010738691582476603	9.204592784979945E-4
24	0.0	0.0	0.0	0.0017642136171211562	9.204592784979945E-4
25	0.0	0.0	0.0	0.0020710333766204877	9.204592784979945E-4
26	0.0	0.0	0.0	0.002607967955744318	9.971642183728275E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTAATA	65	9.437072E-6	19.691906	2
ATACCGT	395	0.0	18.63396	6
TCTAGCG	405	0.0	18.567524	28
TAGCGGC	410	0.0	18.34109	30
CTAGCGG	410	0.0	18.34109	29
TGCGTAC	105	5.980837E-9	18.285341	16
TATTCGG	45	0.008848023	17.778778	5
CCGTGCG	100	6.817754E-8	17.59964	13
TCACGTT	315	0.0	17.26949	27
ACCGTCG	420	0.0	17.143822	8
CGTCGTA	405	0.0	16.98796	10
TACCGTC	430	0.0	16.745129	7
CATCGTT	300	0.0	16.532995	28
CCGTCGT	420	0.0	16.381874	9
ATCGTTT	305	0.0	16.261963	29
TGTAGGA	955	0.0	16.250978	2
GTATTAG	350	0.0	16.001516	1
GACTGCG	70	3.6952866E-4	16.000902	7
GTTATTC	220	0.0	16.000288	3
TCTATAC	80	5.6209246E-5	16.000288	3
>>END_MODULE