FastQCFastQC Report
Thu 2 Feb 2017
SRR4062960_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062960_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201478
Sequences flagged as poor quality0
Sequence length38
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT35561.7649569680064325No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT24741.227925629597276No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA23881.1852410685037573No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC19700.9777742483050258No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA15190.753928468616921No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT13860.6879162985536882No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11230.5573809547444386No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG9880.4903761204697287No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA7870.39061336721627177No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG7580.3762197361498526No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA6860.340483824536674No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA5850.29035428185707624No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA5590.2774496471078728No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG5050.25064771339798886No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC4450.22086778705367335No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA4430.21987512284219615No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4350.21590446599628743No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA4310.21391913757333306No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCT4250.2109411449389015No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4250.2109411449389015No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT3940.19555484966100517No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTA3890.1930731891323122No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC3650.181161218594586No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA3500.17371623700850714No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG3290.16329326278799672No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGG3120.15485561699044065No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA3120.15485561699044065No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT3020.14989229593305473No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA3010.14939596382731613No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC2980.14790696751010035No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAAC2950.14641797119288458No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2820.13996565381828288No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT2790.1384766575010671No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTA2600.12904634749203386No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGAT2560.12706101906907952No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA2540.12606835485760232No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2540.12606835485760232No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTTCT2380.11812704116578486No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA2360.11713437695430766No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCT2320.11514904853135331No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGT2300.11415638431987613No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA2270.11266738800266034No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA2270.11266738800266034No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCT2260.11217105589692174No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCA2250.11167472379118315No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC2240.11117839168544458No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGC2240.11117839168544458No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTC2220.11018572747396739No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGG2200.1091930632624902No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG2190.1086967311567516No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGT2170.10770406694527442No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATG2080.10323707799362708No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAG2080.10323707799362708No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCG2070.10274074588788851No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGC2060.10224441378214992No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCT2020.10025908535919555No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTAGA252.8694523E-432.0325661
TTTAGAA308.391518E-426.6672882
TTAGAAA308.403702E-426.6606663
TAGAAAT308.415901E-426.654054
TCCGGTG557.532253E-826.18242812
ACGATTA509.607975E-725.60059532
CCTTTAG401.5762776E-424.0244221
GCTATTA601.747976E-724.0005599
CGGTGTT401.5869457E-424.00055914
CCGGTGT401.5869457E-424.00055913
TATGGAG401.5923029E-423.9886445
TAGGCAT401.5923029E-423.9886445
GGTTCGT653.7834252E-722.15436218
GAAGCTA653.8015787E-722.1433646
CTGTAGA453.4986564E-421.3550431
CAACGAT604.6877612E-621.3338330
GTTAACA453.5222404E-421.3338323
AACGATT604.6877612E-621.3338331
TTAACAG453.5222404E-421.3338324
TTAGGCA453.5340828E-421.323244
Produced by FastQC (version 0.11.5)