##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062960_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	201478
Sequences flagged as poor quality	0
Sequence length	38
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.770441437774846	32.0	32.0	32.0	32.0	32.0
2	30.59641747486078	32.0	32.0	32.0	32.0	32.0
3	30.757645996088904	32.0	32.0	32.0	32.0	32.0
4	30.693758127438233	32.0	32.0	32.0	32.0	32.0
5	30.816367047518835	32.0	32.0	32.0	32.0	32.0
6	34.344161645440195	36.0	36.0	36.0	32.0	36.0
7	34.07224610131131	36.0	36.0	36.0	32.0	36.0
8	34.02708980633121	36.0	36.0	36.0	32.0	36.0
9	34.01244800921192	36.0	36.0	36.0	32.0	36.0
10	33.97185796960462	36.0	36.0	36.0	32.0	36.0
11	34.29175393839526	36.0	36.0	36.0	32.0	36.0
12	34.134347174381325	36.0	36.0	36.0	32.0	36.0
13	34.17134873286413	36.0	36.0	36.0	32.0	36.0
14	34.092253248493634	36.0	36.0	36.0	32.0	36.0
15	34.12594923515223	36.0	36.0	36.0	32.0	36.0
16	34.08202384379436	36.0	36.0	36.0	32.0	36.0
17	34.030355671586975	36.0	36.0	36.0	32.0	36.0
18	34.088188288547634	36.0	36.0	36.0	32.0	36.0
19	34.01047260743108	36.0	36.0	36.0	32.0	36.0
20	33.85786041155858	36.0	36.0	36.0	32.0	36.0
21	33.7436692839913	36.0	36.0	36.0	32.0	36.0
22	33.772471436087315	36.0	36.0	36.0	32.0	36.0
23	33.75423619452248	36.0	36.0	36.0	32.0	36.0
24	33.744736398018645	36.0	36.0	36.0	27.0	36.0
25	33.693748200796115	36.0	36.0	36.0	27.0	36.0
26	33.69263641687926	36.0	36.0	36.0	27.0	36.0
27	33.63916159580699	36.0	36.0	36.0	27.0	36.0
28	33.64409513693803	36.0	36.0	36.0	27.0	36.0
29	33.575333287009	36.0	36.0	36.0	27.0	36.0
30	33.46895442678605	36.0	36.0	36.0	21.0	36.0
31	33.48576023188636	36.0	36.0	36.0	21.0	36.0
32	33.400063530509534	36.0	36.0	36.0	21.0	36.0
33	33.29078609078907	36.0	36.0	36.0	21.0	36.0
34	33.18293312421207	36.0	36.0	36.0	21.0	36.0
35	33.03628684025055	36.0	36.0	36.0	14.0	36.0
36	32.954913191514706	36.0	36.0	36.0	14.0	36.0
37	32.86948947279604	36.0	36.0	36.0	14.0	36.0
38	32.085944867429696	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	0.0
12	3.0
13	1.0
14	105.0
15	174.0
16	182.0
17	276.0
18	352.0
19	465.0
20	530.0
21	597.0
22	793.0
23	1024.0
24	1431.0
25	1895.0
26	2459.0
27	3383.0
28	4732.0
29	6394.0
30	8721.0
31	10986.0
32	14523.0
33	19953.0
34	40615.0
35	81882.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.38021868787277	18.72813121272366	12.970675944333996	20.920974155069583
2	13.543580829401538	22.528433076732057	40.96250310404768	22.96548298981872
3	18.520504152456972	31.54576638023897	28.728648230055548	21.205081237248507
4	10.176103875476493	17.837714421855146	37.61862690597205	34.36755479669632
5	10.390712633637419	42.116757164553945	33.18823891442242	14.304291287386217
6	27.372851491733545	38.319510415580936	20.528397782377144	13.77924031030837
7	25.975044421723464	34.694606855339046	22.030693177418875	17.299655545518615
8	29.25431064433834	31.40144333376349	20.78489959201501	18.559346429883163
9	29.15702955161358	14.672569709844252	20.734273717229673	35.4361270213125
10	16.693716900688415	28.12182035666602	31.84184795286807	23.342614789777492
11	32.36796259622686	25.276830605974876	22.987239238225705	19.36796755957256
12	23.999384542079454	28.550298794893685	29.732573606781948	17.71774305624491
13	30.06180346992976	22.842463203355752	27.185087741070763	19.910645585643724
14	23.873721830636356	22.58909957311625	28.267149806413183	25.27002878983421
15	24.667619870324586	27.528720224797198	29.388460278216318	18.415199626661902
16	21.68336195856126	28.5340574026825	28.742541721683363	21.04003891707288
17	19.632139399806388	27.462456871943804	29.86918857199593	23.036215156253878
18	20.845287139524498	25.41073112622227	34.006551843946994	19.73742989030625
19	21.49107785471422	28.865063410517955	31.01332737696374	18.630531357804085
20	22.345425937966873	24.613719952549474	34.07404466107121	18.96680944841244
21	24.30571756168504	25.2830488367822	28.528245320573593	21.882988280959164
22	21.778969051696325	28.19447546720274	29.11820911820912	20.908346362891816
23	20.585826396437984	28.322884557155547	30.128711052869317	20.962577993537145
24	19.931198125570866	30.593503038004844	30.055398911878	19.419899924546286
25	22.241251780918482	27.498374213789646	29.694550761761505	20.565823243530364
26	18.954099109385705	27.85775986179096	32.27260541914473	20.915535609678603
27	21.154256612876807	29.841211537602437	30.415014171336672	18.589517678184084
28	19.390672464113408	28.05259396033117	31.45114856949987	21.105585006055552
29	18.93285812145701	29.421604932143318	32.38357143566273	19.261965510736943
30	20.783120570191983	28.39643431475709	31.96360856876253	18.856836546288392
31	19.30071873883175	28.850812055751895	30.514136520668707	21.334332684747647
32	17.580955784541466	30.976631524609367	30.93642662854647	20.505986062302696
33	18.510098628532287	30.388706611139515	30.769421680407817	20.33177307992038
34	17.879197029565354	30.47574608343427	32.64301173480531	19.002045152195063
35	20.224183635984552	30.55916839586581	30.236494871973076	18.980153096176565
36	19.628478668797968	30.850070988175254	30.186852791374193	19.334597551652585
37	20.669714433209734	31.24407447669253	28.79196263259522	19.29424845750252
38	18.693569602660514	33.36261881716427	28.406422951877495	19.53738862829772
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	108.0
1	149.5
2	191.0
3	191.0
4	481.0
5	771.0
6	771.0
7	1046.0
8	1321.0
9	1340.0
10	1359.0
11	1359.0
12	1692.0
13	2025.0
14	2310.5
15	2596.0
16	2596.0
17	3610.5
18	4625.0
19	4625.0
20	5056.5
21	5488.0
22	5043.5
23	4599.0
24	4599.0
25	4670.0
26	4741.0
27	4741.0
28	6400.0
29	8059.0
30	9644.0
31	11229.0
32	11229.0
33	11694.0
34	12159.0
35	12159.0
36	12137.5
37	12116.0
38	12911.0
39	13706.0
40	13706.0
41	14046.0
42	14386.0
43	15627.5
44	16869.0
45	16869.0
46	20113.5
47	23358.0
48	23358.0
49	20095.0
50	16832.0
51	13100.5
52	9369.0
53	9369.0
54	8226.5
55	7084.0
56	7084.0
57	6537.0
58	5990.0
59	5585.5
60	5181.0
61	5181.0
62	4966.5
63	4752.0
64	4408.0
65	4064.0
66	4064.0
67	3543.5
68	3023.0
69	3023.0
70	2588.5
71	2154.0
72	1749.5
73	1345.0
74	1345.0
75	1100.0
76	855.0
77	855.0
78	679.0
79	503.0
80	364.5
81	226.0
82	226.0
83	179.5
84	133.0
85	133.0
86	105.0
87	77.0
88	51.5
89	26.0
90	26.0
91	18.0
92	10.0
93	9.0
94	8.0
95	8.0
96	11.0
97	14.0
98	14.0
99	70.0
100	126.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13798032539532853
2	0.06353050953453976
3	0.015386295277896348
4	0.0029779926344315507
5	0.0
6	4.963321057385919E-4
7	0.0
8	0.0
9	0.0
10	4.963321057385919E-4
11	4.963321057385919E-4
12	9.926642114771837E-4
13	0.01637895948937353
14	0.008933977903294654
15	0.02580926949840677
16	0.011911970537726203
17	0.02134228054675945
18	0.003970656845908735
19	0.0064523173746016925
20	0.0024816605286929593
21	0.00545965316312451
22	0.0064523173746016925
23	0.009430310009033245
24	0.014889963172157756
25	0.018364287912327897
26	0.02183861265249804
27	0.007444981586078878
28	0.004963321057385919
29	0.011911970537726203
30	9.926642114771837E-4
31	0.006948649480340286
32	0.004963321057385919
33	0.007444981586078878
34	0.012904634749203385
35	0.017867955806589307
36	0.017867955806589307
37	0.009430310009033245
38	0.0064523173746016925
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	201478.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.92098392876642
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.37214044161527	29.690090233176825
2	13.141535707181221	10.49246071531383
3	4.795355082554207	5.743058795501246
4	2.1856972349313706	3.490207367553778
5	1.2756116968370799	2.546183702438976
6	0.7559180425701213	1.8106195217343828
7	0.5582355281480008	1.559971808336394
8	0.3841754525561965	1.226932965385799
9	0.28098269345534116	1.0095395030722958
>10	1.8562263775611696	14.81452069208549
>50	0.21260194947284664	6.0095891362828695
>100	0.16411378555798686	12.035557232055112
>500	0.008703003779590213	2.416144690735465
>1k	0.008703003779590213	7.15512363632754
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3556	1.7649569680064325	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2474	1.227925629597276	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2388	1.1852410685037573	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1970	0.9777742483050258	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1519	0.753928468616921	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1386	0.6879162985536882	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1123	0.5573809547444386	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	988	0.4903761204697287	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	787	0.39061336721627177	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	758	0.3762197361498526	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	686	0.340483824536674	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	585	0.29035428185707624	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	559	0.2774496471078728	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	505	0.25064771339798886	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	445	0.22086778705367335	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA	443	0.21987512284219615	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	435	0.21590446599628743	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA	431	0.21391913757333306	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCT	425	0.2109411449389015	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	425	0.2109411449389015	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	394	0.19555484966100517	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTA	389	0.1930731891323122	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	365	0.181161218594586	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	350	0.17371623700850714	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG	329	0.16329326278799672	No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGG	312	0.15485561699044065	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA	312	0.15485561699044065	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT	302	0.14989229593305473	No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA	301	0.14939596382731613	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	298	0.14790696751010035	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAAC	295	0.14641797119288458	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	282	0.13996565381828288	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	279	0.1384766575010671	No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTA	260	0.12904634749203386	No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGAT	256	0.12706101906907952	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA	254	0.12606835485760232	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	254	0.12606835485760232	No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTTCT	238	0.11812704116578486	No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA	236	0.11713437695430766	No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCT	232	0.11514904853135331	No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGT	230	0.11415638431987613	No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA	227	0.11266738800266034	No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA	227	0.11266738800266034	No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCT	226	0.11217105589692174	No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCA	225	0.11167472379118315	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC	224	0.11117839168544458	No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGC	224	0.11117839168544458	No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTC	222	0.11018572747396739	No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGG	220	0.1091930632624902	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	219	0.1086967311567516	No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGT	217	0.10770406694527442	No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATG	208	0.10323707799362708	No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAG	208	0.10323707799362708	No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCG	207	0.10274074588788851	No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGC	206	0.10224441378214992	No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCT	202	0.10025908535919555	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.963321057385918E-4	0.0
10	0.0	0.0	0.0	4.963321057385918E-4	0.0
11	0.0	0.0	0.0	4.963321057385918E-4	0.0
12	0.0	0.0	0.0	9.926642114771835E-4	0.0
13	0.0	0.0	0.0	0.0014889963172157754	0.0
14	0.0	0.0	0.0	0.0014889963172157754	0.0
15	0.0	0.0	0.0	0.0014889963172157754	0.0
16	0.0	0.0	0.0	0.001985328422954367	0.0
17	0.0	0.0	0.0	0.001985328422954367	0.0
18	0.0	0.0	0.0	0.002481660528692959	0.0
19	0.0	0.0	0.0	0.0034743247401701426	0.0
20	0.0	0.0	0.0	0.003970656845908734	0.0
21	0.0	0.0	0.0	0.00545965316312451	0.0
22	0.0	0.0	0.0	0.008933977903294652	0.0
23	0.0	0.0	0.0	0.01637895948937353	0.0
24	0.0	0.0	0.0	0.023823941075452406	0.0
25	0.0	0.0	0.0	0.026801933709883957	0.0
26	0.0	0.0	0.0	0.03275791897874706	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTAGA	25	2.8694523E-4	32.032566	1
TTTAGAA	30	8.391518E-4	26.667288	2
TTAGAAA	30	8.403702E-4	26.660666	3
TAGAAAT	30	8.415901E-4	26.65405	4
TCCGGTG	55	7.532253E-8	26.182428	12
ACGATTA	50	9.607975E-7	25.600595	32
CCTTTAG	40	1.5762776E-4	24.024422	1
GCTATTA	60	1.747976E-7	24.000559	9
CGGTGTT	40	1.5869457E-4	24.000559	14
CCGGTGT	40	1.5869457E-4	24.000559	13
TATGGAG	40	1.5923029E-4	23.988644	5
TAGGCAT	40	1.5923029E-4	23.988644	5
GGTTCGT	65	3.7834252E-7	22.154362	18
GAAGCTA	65	3.8015787E-7	22.143364	6
CTGTAGA	45	3.4986564E-4	21.355043	1
CAACGAT	60	4.6877612E-6	21.33383	30
GTTAACA	45	3.5222404E-4	21.33383	23
AACGATT	60	4.6877612E-6	21.33383	31
TTAACAG	45	3.5222404E-4	21.33383	24
TTAGGCA	45	3.5340828E-4	21.32324	4
>>END_MODULE