FastQCFastQC Report
Thu 2 Feb 2017
SRR4062960_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062960_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201478
Sequences flagged as poor quality0
Sequence length38
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT42402.104448128331629No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT33351.6552675726382038No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA25191.2502605743555126No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC23581.1703511053315996No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT20471.0159918204468974No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13940.691886955399597No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA11570.5742562463395507No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG10090.5007990946902391No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG7730.38366471773593147No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA6700.33254251084485653No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA6350.3151708871440058No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC6340.3146745550382672No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG5860.2908506139628148No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5850.29035428185707624No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA5830.28936161764559903No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA5670.2814203039537816No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA5260.2610706876184993No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5200.2580926949840677No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC5070.251640377609466No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCT5050.25064771339798886No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA5040.2501513812922503No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT4530.2248384438995821No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA4460.22136411915941195No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT4370.21689713020776463No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4300.21342280546759448No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC3860.19158419281509645No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTA3680.18265021491180178No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG3680.18265021491180178No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA3610.17917589017163166No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAAC3380.16776025173964404No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGG3240.16081160225930374No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC3090.15336662067322487No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG2810.1394693217125443No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT2720.135002332760897No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGAT2680.1330170043379426No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTA2630.13053534380924964No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA2590.12855001538629526No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2580.1280536832805567No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA2560.12706101906907952No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA2520.12507569064612514No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGC2370.11763070906004626No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGT2350.11663804484856907No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCT2320.11514904853135331No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA2300.11415638431987613No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCT2300.11415638431987613No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGG2270.11266738800266034No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT2270.11266738800266034No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCA2190.1086967311567516No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGT2170.10770406694527442No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG2150.10671140273379724No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAG2120.10522240641658147No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTTCT2090.10373341009936568No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT2090.10373341009936568No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCC2080.10323707799362708No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACA2050.10174808167641132No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCG2050.10174808167641132No Hit
TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTC2020.10025908535919555No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTACT308.3926745E-426.6666667
AAAAGAT451.2314858E-524.8888919
CAAGTAT651.3898898E-824.61538513
TCTAGCA752.4610927E-923.4666673
ATTTAGA350.002060666422.8571431
AGTATTA703.0522642E-822.85714315
CTACTCA502.7828459E-522.49
AGATAAA502.7828459E-522.422
ATCTAAT453.522804E-421.3333342
AGGAAGT453.522804E-421.33333428
GCATTAC756.3366315E-821.3333347
TGTAGGA1350.021.3333342
TATGTTC453.522804E-421.3333348
CTAATAT555.802766E-520.3636364
GAAATGT400.00447087820.06
ATTACAA801.2525743E-720.09
TTGTAAC400.00447087820.03
GTGCTGG400.00447087820.01
GAAAATT400.00447087820.031
CACGTAG400.00447087820.026
Produced by FastQC (version 0.11.5)