##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062960_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	201478
Sequences flagged as poor quality	0
Sequence length	38
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.60340583090958	32.0	32.0	32.0	32.0	32.0
2	31.273608036609456	32.0	32.0	32.0	32.0	32.0
3	31.36274431947905	32.0	32.0	32.0	32.0	32.0
4	31.51359453637618	32.0	32.0	32.0	32.0	32.0
5	31.354281857076206	32.0	32.0	32.0	32.0	32.0
6	34.81983144561689	36.0	36.0	36.0	32.0	36.0
7	34.89802856887601	36.0	36.0	36.0	36.0	36.0
8	34.88970507946277	36.0	36.0	36.0	36.0	36.0
9	34.945860093906035	36.0	36.0	36.0	32.0	36.0
10	34.823881515599716	36.0	36.0	36.0	32.0	36.0
11	34.98037006521804	36.0	36.0	36.0	36.0	36.0
12	34.92143062766158	36.0	36.0	36.0	32.0	36.0
13	34.92563456059719	36.0	36.0	36.0	36.0	36.0
14	34.864079452843484	36.0	36.0	36.0	32.0	36.0
15	34.84877257070251	36.0	36.0	36.0	32.0	36.0
16	34.81297213591559	36.0	36.0	36.0	32.0	36.0
17	34.79458303139797	36.0	36.0	36.0	32.0	36.0
18	34.768674495478415	36.0	36.0	36.0	32.0	36.0
19	34.73649728506338	36.0	36.0	36.0	32.0	36.0
20	34.671626678843346	36.0	36.0	36.0	32.0	36.0
21	34.60430915534202	36.0	36.0	36.0	32.0	36.0
22	34.610379296995205	36.0	36.0	36.0	32.0	36.0
23	34.560071074757545	36.0	36.0	36.0	32.0	36.0
24	34.50844260911861	36.0	36.0	36.0	32.0	36.0
25	34.50224342111794	36.0	36.0	36.0	32.0	36.0
26	34.40655059113154	36.0	36.0	36.0	32.0	36.0
27	34.369449766227575	36.0	36.0	36.0	32.0	36.0
28	34.30079710936182	36.0	36.0	36.0	32.0	36.0
29	34.248890697743676	36.0	36.0	36.0	32.0	36.0
30	34.188000675011665	36.0	36.0	36.0	32.0	36.0
31	34.11398763140392	36.0	36.0	36.0	32.0	36.0
32	34.05310753531403	36.0	36.0	36.0	32.0	36.0
33	33.92220986906759	36.0	36.0	36.0	32.0	36.0
34	33.82224361965078	36.0	36.0	36.0	32.0	36.0
35	33.66140223746513	36.0	36.0	36.0	27.0	36.0
36	33.57310971917529	36.0	36.0	36.0	27.0	36.0
37	33.47529755109739	36.0	36.0	36.0	21.0	36.0
38	33.055638829053294	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	4.0
21	7.0
22	14.0
23	64.0
24	172.0
25	411.0
26	873.0
27	1819.0
28	3147.0
29	4972.0
30	7427.0
31	10627.0
32	14349.0
33	21022.0
34	45037.0
35	91532.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.67630970227045	20.08408700572824	13.732887251933407	21.506716040067904
2	13.003404838245366	23.65369916318407	39.61375435531423	23.729141643256334
3	17.94439095087305	31.234675746235318	29.63499736943984	21.18593593345179
4	9.97131200428831	18.416899115536186	37.57184407230566	34.039944807869844
5	10.13659059549926	41.789674306872215	33.4979501484033	14.575784949225227
6	27.612940370660816	38.64739574544119	19.658722044094144	14.080941839803849
7	25.20499126429479	34.64253097204574	22.3058290978399	17.84664866581957
8	28.951339117314223	31.8633484881574	20.7399392483472	18.445373146181183
9	28.213846092757304	14.313651524777637	20.949313055908515	36.52318932655654
10	16.352238717074403	28.35162326220859	32.15205705862208	23.14408096209493
11	32.42846151937061	24.734321097957462	22.931030203757476	19.906187178914454
12	23.1692790279832	29.03840617834205	30.16756171889735	17.624753074777395
13	30.256901497930293	22.354301710360435	27.66852956650354	19.720267225205728
14	23.46707829142636	22.335937422448108	28.857741291853205	25.339242994272325
15	24.805685980603343	27.39703590466453	29.459792136114118	18.337485978618012
16	21.700632327102714	27.867558740904713	29.084565064175745	21.347243867816836
17	19.37184208697724	27.16425614707313	30.5785247024489	22.88537706350073
18	20.789862913072398	24.278581284308956	35.07380458412333	19.85775121849532
19	21.532872075363066	28.085944867429696	32.239748260355974	18.141434796851268
20	21.75076186978231	24.105857711511927	35.44307567079284	18.700304747912924
21	23.69600500305246	24.85842056413387	29.825736932751628	21.61983750006204
22	21.41921202314893	27.169219468130517	30.490673919733176	20.92089458898738
23	20.232481958327956	27.133483556517334	31.44561689117422	21.188417593980486
24	19.820526310564926	29.512899671428144	31.20787381252544	19.45870020548149
25	22.027218852678704	25.978022414357895	31.391516691648718	20.603242041314683
26	18.871042992287	26.56220530281222	33.67712603857493	20.889625666325852
27	21.093121829678672	28.448267304618867	32.02930344752281	18.429307418179654
28	19.484509474979898	26.246041751456733	33.14356902490594	21.12587974865742
29	18.985695708712612	27.53799422269429	33.94861970041394	19.527690368179158
30	20.963082817975163	26.574117273349945	33.486038177865574	18.97676173080932
31	19.294910610587756	27.054070419599164	32.219894976126426	21.431123993686654
32	17.786557341248177	28.942117749828768	32.48890697743674	20.782417931486314
33	18.69385243053832	28.692959032747993	31.99604919643832	20.617139340275365
34	18.135478811582406	28.728198612255433	34.081140372646146	19.055182203516015
35	20.665283554532007	28.71628664171771	31.696760936677947	18.921668867072334
36	19.610081497731763	29.025501543592846	31.871469837897937	19.492947120777455
37	21.03951796225891	29.014085905160865	30.415727771766644	19.53066836081359
38	19.288954625318894	30.517475853443056	29.94470860342072	20.24886091781733
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	51.0
1	66.5
2	82.0
3	82.0
4	399.5
5	717.0
6	717.0
7	999.5
8	1282.0
9	1411.5
10	1541.0
11	1541.0
12	1918.5
13	2296.0
14	2585.0
15	2874.0
16	2874.0
17	4196.5
18	5519.0
19	5519.0
20	5732.5
21	5946.0
22	5441.0
23	4936.0
24	4936.0
25	4687.5
26	4439.0
27	4439.0
28	6019.0
29	7599.0
30	9033.5
31	10468.0
32	10468.0
33	11197.0
34	11926.0
35	11926.0
36	11828.0
37	11730.0
38	12373.5
39	13017.0
40	13017.0
41	13178.0
42	13339.0
43	14625.5
44	15912.0
45	15912.0
46	19995.5
47	24079.0
48	24079.0
49	21244.5
50	18410.0
51	14134.0
52	9858.0
53	9858.0
54	8591.0
55	7324.0
56	7324.0
57	6583.5
58	5843.0
59	5493.0
60	5143.0
61	5143.0
62	4973.0
63	4803.0
64	4364.0
65	3925.0
66	3925.0
67	3472.0
68	3019.0
69	3019.0
70	2649.5
71	2280.0
72	1810.0
73	1340.0
74	1340.0
75	1109.5
76	879.0
77	879.0
78	667.0
79	455.0
80	356.5
81	258.0
82	258.0
83	180.5
84	103.0
85	103.0
86	74.5
87	46.0
88	31.5
89	17.0
90	17.0
91	13.0
92	9.0
93	8.0
94	7.0
95	7.0
96	6.5
97	6.0
98	6.0
99	3.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009926642114771837
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0029779926344315507
8	9.926642114771837E-4
9	0.0029779926344315507
10	4.963321057385919E-4
11	0.0064523173746016925
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	4.963321057385919E-4
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	201478.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.80352197262232
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.15142074955159	26.554263989120404
2	14.234467505495543	10.477570752141672
3	5.124678021874283	5.658186005419947
4	2.4922118380062304	3.668886925619671
5	1.3661312726733288	2.5139221155659675
6	0.8846812585130343	1.9535631681870975
7	0.5529257865706464	1.4244731434697586
8	0.4005340453938585	1.1792850832348942
9	0.31422367870966	1.040808425733827
>10	2.028293617078664	14.843307954218327
>50	0.2521881026553924	6.568459087344523
>100	0.16992353440951571	11.38436950932608
>500	0.017531793232727813	3.7696423430846044
>1k	0.010788795835524808	8.96326149753323
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4240	2.104448128331629	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3335	1.6552675726382038	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2519	1.2502605743555126	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	2358	1.1703511053315996	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2047	1.0159918204468974	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1394	0.691886955399597	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1157	0.5742562463395507	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	1009	0.5007990946902391	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	773	0.38366471773593147	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	670	0.33254251084485653	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	635	0.3151708871440058	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	634	0.3146745550382672	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	586	0.2908506139628148	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	585	0.29035428185707624	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	583	0.28936161764559903	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	567	0.2814203039537816	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA	526	0.2610706876184993	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	520	0.2580926949840677	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	507	0.251640377609466	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCT	505	0.25064771339798886	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA	504	0.2501513812922503	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	453	0.2248384438995821	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	446	0.22136411915941195	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT	437	0.21689713020776463	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	430	0.21342280546759448	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	386	0.19158419281509645	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTA	368	0.18265021491180178	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG	368	0.18265021491180178	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA	361	0.17917589017163166	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAAC	338	0.16776025173964404	No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGG	324	0.16081160225930374	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC	309	0.15336662067322487	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	281	0.1394693217125443	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	272	0.135002332760897	No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGAT	268	0.1330170043379426	No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTA	263	0.13053534380924964	No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA	259	0.12855001538629526	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	258	0.1280536832805567	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA	256	0.12706101906907952	No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA	252	0.12507569064612514	No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGC	237	0.11763070906004626	No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGT	235	0.11663804484856907	No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCT	232	0.11514904853135331	No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA	230	0.11415638431987613	No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCT	230	0.11415638431987613	No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGG	227	0.11266738800266034	No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT	227	0.11266738800266034	No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCA	219	0.1086967311567516	No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGT	217	0.10770406694527442	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	215	0.10671140273379724	No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAG	212	0.10522240641658147	No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTTCT	209	0.10373341009936568	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	209	0.10373341009936568	No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCC	208	0.10323707799362708	No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACA	205	0.10174808167641132	No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCG	205	0.10174808167641132	No Hit
TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTC	202	0.10025908535919555	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	4.963321057385918E-4	0.0
13	0.0	0.0	0.0	9.926642114771835E-4	0.0
14	0.0	0.0	0.0	9.926642114771835E-4	0.0
15	0.0	0.0	0.0	9.926642114771835E-4	0.0
16	0.0	0.0	0.0	0.0014889963172157754	0.0
17	0.0	0.0	0.0	0.0014889963172157754	0.0
18	0.0	0.0	0.0	0.002481660528692959	0.0
19	0.0	0.0	0.0	0.0029779926344315507	0.0
20	0.0	0.0	0.0	0.0034743247401701426	0.0
21	0.0	0.0	0.0	0.004963321057385918	0.0
22	0.0	0.0	0.0	0.00843764579755606	0.0
23	0.0	0.0	0.0	0.015386295277896346	0.0
24	0.0	0.0	0.0	0.023327608969713816	0.0
25	0.0	0.0	0.0	0.026305601604145367	0.0
26	0.0	0.0	0.0	0.03275791897874706	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACTACT	30	8.3926745E-4	26.666666	7
AAAAGAT	45	1.2314858E-5	24.88889	19
CAAGTAT	65	1.3898898E-8	24.615385	13
TCTAGCA	75	2.4610927E-9	23.466667	3
ATTTAGA	35	0.0020606664	22.857143	1
AGTATTA	70	3.0522642E-8	22.857143	15
CTACTCA	50	2.7828459E-5	22.4	9
AGATAAA	50	2.7828459E-5	22.4	22
ATCTAAT	45	3.522804E-4	21.333334	2
AGGAAGT	45	3.522804E-4	21.333334	28
GCATTAC	75	6.3366315E-8	21.333334	7
TGTAGGA	135	0.0	21.333334	2
TATGTTC	45	3.522804E-4	21.333334	8
CTAATAT	55	5.802766E-5	20.363636	4
GAAATGT	40	0.004470878	20.0	6
ATTACAA	80	1.2525743E-7	20.0	9
TTGTAAC	40	0.004470878	20.0	3
GTGCTGG	40	0.004470878	20.0	1
GAAAATT	40	0.004470878	20.0	31
CACGTAG	40	0.004470878	20.0	26
>>END_MODULE