FastQCFastQC Report
Thu 2 Feb 2017
SRR4062959_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062959_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2782516
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT123430.4435913396365016No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT76860.27622482673954074No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT47190.16959471212384764No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG38080.1368545589674956No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA37080.13326068924671053No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC34970.125677624135854No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT33010.11863363948311528No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG32450.11662107243947564No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA32170.11561478891765582No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG31320.1125599996549885No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG29760.1069535628905638No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT29430.10576758588270471No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT29000.10422222190276714No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA28980.10415034450835144No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC27990.10059241348477421No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC27950.10044865869594281No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG27910.1003049039071114No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGTT6550.015.87683628
CGAGCCG10200.015.84467715
ATCGTTT7000.014.85671729
TGGATAC11450.014.53285724
GTCTAAT1452.0165317E-814.3539631
TACCGTC6500.014.2757457
CGGCATC7650.014.22232525
ACCGCAG11900.013.98279429
AGGCCCG11450.013.97490510
CGTTTAT7500.013.8662731
GTATTAT2550.013.8127121
GTATTAG7150.013.6590421
ATACCGT6450.013.6403256
TCTAGCG7650.013.59389328
GTATAAT3200.013.5085961
ACCGTCG6550.013.4340058
GTTATAT3850.013.307171
GCATTCG10000.013.28009722
AGAACCG7000.013.2539045
GTTCAAA13650.013.2538561
Produced by FastQC (version 0.11.5)