##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062959_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2782516
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.161654775749717	32.0	32.0	32.0	32.0	32.0
2	30.738673200801003	32.0	32.0	32.0	32.0	32.0
3	30.799628106361293	32.0	32.0	32.0	32.0	32.0
4	30.870058249440433	32.0	32.0	32.0	32.0	32.0
5	30.772745601462848	32.0	32.0	32.0	32.0	32.0
6	34.42815207531601	36.0	36.0	36.0	32.0	36.0
7	34.32758697524111	36.0	36.0	36.0	32.0	36.0
8	34.31390115995739	36.0	36.0	36.0	32.0	36.0
9	34.456656853006415	36.0	36.0	36.0	32.0	36.0
10	34.15717537652973	36.0	36.0	36.0	32.0	36.0
11	34.47490149203095	36.0	36.0	36.0	32.0	36.0
12	34.26672694784145	36.0	36.0	36.0	32.0	36.0
13	34.36835259887095	36.0	36.0	36.0	32.0	36.0
14	34.26579721374468	36.0	36.0	36.0	32.0	36.0
15	34.20487932504251	36.0	36.0	36.0	32.0	36.0
16	34.216215468302785	36.0	36.0	36.0	32.0	36.0
17	34.135809821039665	36.0	36.0	36.0	32.0	36.0
18	34.163737782639885	36.0	36.0	36.0	32.0	36.0
19	34.16071713513956	36.0	36.0	36.0	32.0	36.0
20	34.12897140573495	36.0	36.0	36.0	32.0	36.0
21	34.117665091593366	36.0	36.0	36.0	32.0	36.0
22	34.08717254456039	36.0	36.0	36.0	32.0	36.0
23	34.04499381135634	36.0	36.0	36.0	32.0	36.0
24	34.026825362369884	36.0	36.0	36.0	32.0	36.0
25	34.00819581989825	36.0	36.0	36.0	32.0	36.0
26	33.957421269096024	36.0	36.0	36.0	32.0	36.0
27	33.96942515335042	36.0	36.0	36.0	32.0	36.0
28	33.95542020243549	36.0	36.0	36.0	32.0	36.0
29	33.918629398716845	36.0	36.0	36.0	32.0	36.0
30	33.890579245546114	36.0	36.0	36.0	32.0	36.0
31	33.911813984178345	36.0	36.0	36.0	32.0	36.0
32	33.862913636435515	36.0	36.0	36.0	32.0	36.0
33	33.81185732624718	36.0	36.0	36.0	32.0	36.0
34	33.8148535354334	36.0	36.0	36.0	32.0	36.0
35	33.771314881927005	36.0	36.0	36.0	32.0	36.0
36	33.72630885141361	36.0	36.0	36.0	27.0	36.0
37	33.72510742076595	36.0	36.0	36.0	27.0	36.0
38	33.11966687702784	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	5.0
8	4.0
9	7.0
10	29.0
11	25.0
12	9.0
13	17.0
14	453.0
15	996.0
16	1296.0
17	1409.0
18	1873.0
19	2349.0
20	3103.0
21	4286.0
22	6334.0
23	9710.0
24	14249.0
25	20833.0
26	30172.0
27	42714.0
28	58559.0
29	77923.0
30	104002.0
31	138092.0
32	186540.0
33	276785.0
34	600097.0
35	1200643.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.99914001616338	17.76535898747878	11.898757953051359	25.33674304330648
2	16.459895563272966	20.309067124437348	37.59653852875246	25.63449878353723
3	18.049247570617084	25.357164936373817	28.991283119616647	27.60230437339245
4	12.446135367616456	15.836687106774704	36.50769576896233	35.20948175664651
5	14.399927834862588	36.791389922828586	33.400311302002116	15.408370940306709
6	32.91603579074554	36.19444623140211	17.32541912742717	13.564098850425173
7	29.323543628695624	31.145744048100372	21.670862511073615	17.859849812130392
8	28.17903292177373	32.160768803649944	19.981239925894112	19.67895834868221
9	28.05021365601561	14.051845276387148	19.070767041268493	38.82717402632875
10	16.427672955974842	26.378292902066487	31.193998203054807	26.000035938903864
11	36.70348666914649	21.653755406646287	22.62519968876406	19.01755823544316
12	24.901956415170016	24.220035968482296	28.56270548540919	22.315302130938502
13	29.131329469380418	19.706519297299728	25.59216426292852	25.56998697039134
14	24.146594284366977	19.968629864024408	25.361719808041222	30.523056043567397
15	25.020058550838005	26.754512095521836	23.15295975061254	25.07246960302762
16	25.559771232057415	25.825639204545453	23.912087090541036	24.702502472856093
17	23.713239787372014	25.870397289956532	25.41607535439196	25.000287568279493
18	24.880865823954927	24.605884126716568	26.60393111332905	23.90931893599946
19	25.413974050246203	25.01865363188729	26.086547101319052	23.48082521654746
20	25.776495738168727	23.906221022925763	25.43599489931612	24.881288339589386
21	27.231937479873025	23.96547361641441	24.85030564245296	23.952283261259602
22	25.96303589700829	24.007689982572106	25.25495800089206	24.774316119527544
23	24.158523383216185	23.935610433562452	26.085990772942207	25.819875410279153
24	24.58959472748917	25.27561636272666	25.49753282379531	24.637256085988852
25	24.751297608948832	24.418951560725226	25.75426665324726	25.07548417707869
26	24.49021185921296	25.263363730050692	26.19722795453181	24.049196456204534
27	25.232543094152998	24.892138821398277	25.075944908492314	24.79937317595641
28	24.236930737301467	24.71296735240619	26.295766634595445	24.7543352756969
29	24.27144405754262	24.985479200752206	26.112961754227566	24.630114987477608
30	24.23106836034088	25.11759496728042	26.392702499108694	24.258634173270003
31	24.869067420753982	24.935162675553165	25.07177391181836	25.123995991874487
32	24.406405995548337	25.050398388976724	24.981319620131465	25.561875995343474
33	24.076892167088502	24.631975289384343	25.716587007949578	25.57454553557758
34	25.005804804724928	24.650363851629912	25.897013059552627	24.446818284092533
35	25.775526701813067	24.59195054630048	25.65090757732442	23.981615174562027
36	24.45682433231456	25.453549480951693	25.125273578228907	24.964352608504836
37	25.46793877628039	25.260157677854835	25.133210291651913	24.13869325421286
38	24.259419928362615	25.119197971783525	25.74679784613416	24.874584253719703
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1300.0
1	1106.5
2	913.0
3	913.0
4	2019.0
5	3125.0
6	3125.0
7	3598.5
8	4072.0
9	3901.0
10	3730.0
11	3730.0
12	4885.5
13	6041.0
14	7599.0
15	9157.0
16	9157.0
17	13578.0
18	17999.0
19	17999.0
20	21628.0
21	25257.0
22	25326.0
23	25395.0
24	25395.0
25	27953.0
26	30511.0
27	30511.0
28	38356.5
29	46202.0
30	55613.0
31	65024.0
32	65024.0
33	80462.5
34	95901.0
35	95901.0
36	106513.5
37	117126.0
38	133488.0
39	149850.0
40	149850.0
41	161039.0
42	172228.0
43	196975.0
44	221722.0
45	221722.0
46	226145.5
47	230569.0
48	230569.0
49	243846.0
50	257123.0
51	256247.5
52	255372.0
53	255372.0
54	243161.0
55	230950.0
56	230950.0
57	224020.5
58	217091.0
59	194690.0
60	172289.0
61	172289.0
62	163326.0
63	154363.0
64	126709.0
65	99055.0
66	99055.0
67	83103.5
68	67152.0
69	67152.0
70	53574.5
71	39997.0
72	31222.0
73	22447.0
74	22447.0
75	16831.5
76	11216.0
77	11216.0
78	10827.5
79	10439.0
80	8119.5
81	5800.0
82	5800.0
83	5985.5
84	6171.0
85	6171.0
86	4141.5
87	2112.0
88	1844.5
89	1577.0
90	1577.0
91	1160.0
92	743.0
93	664.5
94	586.0
95	586.0
96	544.5
97	503.0
98	503.0
99	955.5
100	1408.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12197593832344542
2	0.053979923206191806
3	0.01178789268417504
4	0.0028032183822123574
5	3.234482748706566E-4
6	3.234482748706566E-4
7	3.593869720785074E-5
8	3.9532566928635813E-4
9	2.1563218324710445E-4
10	5.750191553256118E-4
11	0.002192260529678895
12	0.002875095776628059
13	0.014052030608269636
14	0.009056551696378385
15	0.023719540157181487
16	0.011069118740018027
17	0.02041318001405922
18	0.00535486588396976
19	0.007762758596895759
20	0.005175172397930506
21	0.006468965497413133
22	0.007008045955530893
23	0.009487816062872594
24	0.013944214516646087
25	0.01710681987093695
26	0.018939693428537338
27	0.008481532541052774
28	0.006001762433711073
29	0.01078160916235522
30	0.003018850565459462
31	0.006037701130918924
32	0.006648658983452386
33	0.00826590035780567
34	0.012147279656253548
35	0.01577708807424647
36	0.014555172369179548
37	0.009379999971249041
38	0.006109578525334626
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2782516.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.706550098692624
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.16391357542375	32.709061676760996
2	14.620745734921442	13.072862033570237
3	5.2266725081448	7.009994890045047
4	2.40667764106252	4.303770181262598
5	1.258018161599753	2.8120825983312274
6	0.7665483368806487	2.0561838975494537
7	0.518228345336493	1.6217741038343954
8	0.3603742029887829	1.2888869888155559
9	0.24498093937775287	0.985702737556466
>10	1.2350039354882376	9.679849674145455
>50	0.08582540027380886	2.6761989121803604
>100	0.08721491454087116	8.585249415156534
>500	0.016042076525945026	5.0192201004884325
>1k	0.009593000535612144	7.457327797895502
>5k	8.061344987909365E-5	0.2769995382519166
>10k+	8.061344987909365E-5	0.4448354541560508
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	12343	0.4435913396365016	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	7686	0.27622482673954074	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	4719	0.16959471212384764	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3808	0.1368545589674956	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	3708	0.13326068924671053	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	3497	0.125677624135854	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3301	0.11863363948311528	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	3245	0.11662107243947564	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	3217	0.11561478891765582	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	3132	0.1125599996549885	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2976	0.1069535628905638	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	2943	0.10576758588270471	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	2900	0.10422222190276714	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	2898	0.10415034450835144	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2799	0.10059241348477421	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2795	0.10044865869594281	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2791	0.1003049039071114	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.1563218324710442E-4
2	0.0	0.0	0.0	3.593869720785074E-5	2.1563218324710442E-4
3	0.0	0.0	0.0	3.593869720785074E-5	2.1563218324710442E-4
4	0.0	0.0	0.0	7.187739441570148E-5	2.1563218324710442E-4
5	0.0	0.0	0.0	7.187739441570148E-5	2.1563218324710442E-4
6	0.0	0.0	0.0	7.187739441570148E-5	2.875095776628059E-4
7	0.0	0.0	0.0	7.187739441570148E-5	2.875095776628059E-4
8	0.0	0.0	0.0	7.187739441570148E-5	2.875095776628059E-4
9	0.0	0.0	0.0	1.4375478883140296E-4	2.875095776628059E-4
10	0.0	0.0	0.0	1.4375478883140296E-4	3.234482748706566E-4
11	0.0	0.0	0.0	1.4375478883140296E-4	3.234482748706566E-4
12	0.0	0.0	0.0	1.796934860392537E-4	5.39080458117761E-4
13	0.0	0.0	0.0	1.796934860392537E-4	7.187739441570147E-4
14	0.0	0.0	0.0	1.796934860392537E-4	7.187739441570147E-4
15	0.0	0.0	0.0	1.796934860392537E-4	8.625287329884177E-4
16	0.0	0.0	0.0	1.796934860392537E-4	9.344061274041192E-4
17	0.0	0.0	0.0	1.796934860392537E-4	9.344061274041192E-4
18	0.0	0.0	0.0	2.5157088045495515E-4	0.0010062835218198206
19	0.0	0.0	0.0	2.5157088045495515E-4	0.0010422222190276714
20	0.0	0.0	0.0	3.234482748706566E-4	0.001221915705066925
21	0.0	0.0	0.0	3.593869720785074E-4	0.0012937930994826265
22	0.0	0.0	0.0	6.109578525334625E-4	0.0013297317966904773
23	0.0	0.0	0.0	0.0012578544022747759	0.0013297317966904773
24	0.0	0.0	0.0	0.002156321832471044	0.001365670493898328
25	0.0	0.0	0.0	0.0026594635933809545	0.001365670493898328
26	0.0	0.0	0.0	0.00341417623474582	0.0015453639799375817
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATCGTT	655	0.0	15.876836	28
CGAGCCG	1020	0.0	15.844677	15
ATCGTTT	700	0.0	14.856717	29
TGGATAC	1145	0.0	14.532857	24
GTCTAAT	145	2.0165317E-8	14.353963	1
TACCGTC	650	0.0	14.275745	7
CGGCATC	765	0.0	14.222325	25
ACCGCAG	1190	0.0	13.982794	29
AGGCCCG	1145	0.0	13.974905	10
CGTTTAT	750	0.0	13.86627	31
GTATTAT	255	0.0	13.812712	1
GTATTAG	715	0.0	13.659042	1
ATACCGT	645	0.0	13.640325	6
TCTAGCG	765	0.0	13.593893	28
GTATAAT	320	0.0	13.508596	1
ACCGTCG	655	0.0	13.434005	8
GTTATAT	385	0.0	13.30717	1
GCATTCG	1000	0.0	13.280097	22
AGAACCG	700	0.0	13.253904	5
GTTCAAA	1365	0.0	13.253856	1
>>END_MODULE