FastQCFastQC Report
Thu 2 Feb 2017
SRR4062959_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062959_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2782516
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT139690.5020276612964669No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT97980.3521273552425215No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT61730.2218495778640626No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT40150.1442938692895207No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG40130.144221991895105No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA36690.13185908005560434No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC36590.13149969308352583No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA34690.1246713406140342No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG32620.1172320302920091No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG32090.11532727933999301No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA31640.11371003796563973No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT30310.10893019123699559No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG29810.10713325637660305No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC29620.10645042112965389No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC29330.10540819891062621No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT29110.1046175475720535No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC28720.10321593838094731No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCGT550.001365737517.45372225
ATCGTTT5700.017.40268729
ACCGTCG6850.016.586148
CGTCGTA6850.016.35076910
CTAGCGG7150.016.11112829
TCTAGCG7200.015.99924628
GTATAGG2550.015.6877911
CCGTCGT7200.015.5567799
TACCGTC7400.015.3536617
CGTTTAT6550.015.14432331
ATACCGT7800.014.9766136
CGGCATC6750.014.93262825
TAGCGGC7750.014.86381330
CGGACGA650.004164861514.76853426
GTTTTCG9200.014.43410128
CATCGTT6800.014.35226428
GTCGTAG7700.014.33775911
TCGTTTA6950.014.27270830
TACCGTA901.53346E-414.2243647
GTCCTAT10200.013.9621331
Produced by FastQC (version 0.11.5)