##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062959_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2782516
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.148639935942867	32.0	32.0	32.0	32.0	32.0
2	31.291358612133767	32.0	32.0	32.0	32.0	32.0
3	31.37305230230482	32.0	32.0	32.0	32.0	32.0
4	31.484320305795187	32.0	32.0	32.0	32.0	32.0
5	31.402937485354983	32.0	32.0	32.0	32.0	32.0
6	34.9201449335781	36.0	36.0	36.0	36.0	36.0
7	34.94810344307095	36.0	36.0	36.0	36.0	36.0
8	34.89598262867132	36.0	36.0	36.0	36.0	36.0
9	34.99266563067382	36.0	36.0	36.0	36.0	36.0
10	34.84965944490526	36.0	36.0	36.0	32.0	36.0
11	35.030408091094536	36.0	36.0	36.0	36.0	36.0
12	34.91755339412244	36.0	36.0	36.0	32.0	36.0
13	34.970404842236306	36.0	36.0	36.0	36.0	36.0
14	34.91486374202341	36.0	36.0	36.0	32.0	36.0
15	34.89002578960912	36.0	36.0	36.0	32.0	36.0
16	34.8978981612325	36.0	36.0	36.0	32.0	36.0
17	34.86268147245155	36.0	36.0	36.0	32.0	36.0
18	34.85634080810317	36.0	36.0	36.0	32.0	36.0
19	34.857171710782616	36.0	36.0	36.0	32.0	36.0
20	34.840717178266004	36.0	36.0	36.0	32.0	36.0
21	34.82870862198097	36.0	36.0	36.0	32.0	36.0
22	34.81683842968019	36.0	36.0	36.0	32.0	36.0
23	34.76106049345269	36.0	36.0	36.0	32.0	36.0
24	34.736312028394444	36.0	36.0	36.0	32.0	36.0
25	34.72047204760008	36.0	36.0	36.0	32.0	36.0
26	34.65475382711187	36.0	36.0	36.0	32.0	36.0
27	34.64130916048641	36.0	36.0	36.0	32.0	36.0
28	34.611338802723864	36.0	36.0	36.0	32.0	36.0
29	34.58055694917837	36.0	36.0	36.0	32.0	36.0
30	34.55596266113115	36.0	36.0	36.0	32.0	36.0
31	34.547114913265546	36.0	36.0	36.0	32.0	36.0
32	34.516199008379466	36.0	36.0	36.0	32.0	36.0
33	34.472989553339495	36.0	36.0	36.0	32.0	36.0
34	34.45474491431496	36.0	36.0	36.0	32.0	36.0
35	34.419187886071455	36.0	36.0	36.0	32.0	36.0
36	34.38783891988402	36.0	36.0	36.0	32.0	36.0
37	34.38202224174093	36.0	36.0	36.0	32.0	36.0
38	33.95037548750843	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	6.0
20	19.0
21	77.0
22	242.0
23	716.0
24	1924.0
25	4566.0
26	9953.0
27	19021.0
28	32915.0
29	52090.0
30	77525.0
31	111384.0
32	159826.0
33	254979.0
34	618912.0
35	1438359.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.3704202627842	18.920505211008372	12.676029002082103	26.03304552412533
2	15.523360871959046	21.264891199188074	36.78577230103978	26.425975627813102
3	17.110629372840982	26.027235782291996	29.296902515565048	27.56523232930197
4	11.96515953929399	16.692866014594006	36.53472114405976	34.807253302052246
5	13.904183804939057	37.23767886246795	33.19371144450255	15.664425888090449
6	32.90355412407629	36.632987291348755	16.918908584107754	13.544550000467204
7	28.996036299717414	31.289009419942705	21.699794537360454	18.015159742979428
8	27.997287274015875	32.363669166749624	19.844553756935007	19.79448980229949
9	27.851333143330116	13.797144848223247	18.96062009575156	39.39090191269508
10	16.18060252594263	26.58007982789491	31.30531474372198	25.93400290244048
11	36.77723479875373	21.500459856950165	22.612706284208485	19.109599060087614
12	24.673334037734328	24.319120965058115	28.59411623910245	22.413428758105102
13	29.14529358554198	19.60743371724317	25.70279147144432	25.54448122577054
14	23.984767742575425	19.794387525534443	25.457427738061522	30.763416993828606
15	24.998203065139606	26.8178152434703	23.03993939298103	25.144042298409065
16	25.42138122476205	25.767686511056898	23.96043724456571	24.85049501961534
17	23.6829905021211	25.87740735363247	25.414552872292557	25.02504927195387
18	24.87256137970096	24.4561756338508	26.642290646307153	24.02897234014108
19	25.39291777657343	24.868500306916474	26.219040609290296	23.519541307219797
20	25.6495919520319	23.74789578927848	25.744721683541083	24.857790575148535
21	27.102603220467813	23.971407162225542	24.992210284580676	23.93377933272597
22	25.739035181506786	24.047291063869245	25.478299652148973	24.735374102474992
23	24.044551078628896	23.699431521278957	26.34858261270204	25.90743478739011
24	24.49229402454469	25.017897471209512	25.81516153006847	24.674646974177328
25	24.64641687337422	24.223094654689966	26.021324600702815	25.109163871232994
26	24.556875226414117	25.03123077276935	26.330811870712505	24.08108213010402
27	25.238884520340584	24.62275149540919	25.254697547112038	24.88366643713819
28	24.110660998894527	24.448951955712026	26.594779688598376	24.845607356795075
29	24.268503757031407	24.686866131227998	26.385364899968227	24.659265211772365
30	24.231091573238032	24.710945058357257	26.69601899863289	24.36194436977182
31	24.81624544117626	24.599571035710127	25.352702374397847	25.23148114871577
32	24.407119312162088	24.733262989323332	25.324095171420396	25.535522527094184
33	24.02451594168731	24.33984207098899	26.047792717094886	25.587849270228812
34	24.91586750983642	24.346957933036144	26.244880532582744	24.49229402454469
35	25.736743292760938	24.253481381598522	25.910435016366485	24.099340309274055
36	24.506777319519458	25.08179647484507	25.469107814654073	24.9423183909814
37	25.458973102041462	24.904978084582442	25.44308819787559	24.192960615500503
38	24.294441751035844	24.751762166070744	26.016805671707555	24.93699041118586
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	657.0
1	603.5
2	550.0
3	550.0
4	1809.5
5	3069.0
6	3069.0
7	3646.5
8	4224.0
9	4070.5
10	3917.0
11	3917.0
12	5078.0
13	6239.0
14	7933.5
15	9628.0
16	9628.0
17	14813.5
18	19999.0
19	19999.0
20	23182.5
21	26366.0
22	26272.0
23	26178.0
24	26178.0
25	28004.5
26	29831.0
27	29831.0
28	37519.5
29	45208.0
30	54615.0
31	64022.0
32	64022.0
33	80245.0
34	96468.0
35	96468.0
36	106656.0
37	116844.0
38	133585.5
39	150327.0
40	150327.0
41	161433.0
42	172539.0
43	197267.5
44	221996.0
45	221996.0
46	226139.0
47	230282.0
48	230282.0
49	244724.5
50	259167.0
51	257868.5
52	256570.0
53	256570.0
54	243396.5
55	230223.0
56	230223.0
57	224145.0
58	218067.0
59	195358.5
60	172650.0
61	172650.0
62	163454.0
63	154258.0
64	126939.0
65	99620.0
66	99620.0
67	83326.5
68	67033.0
69	67033.0
70	53763.0
71	40493.0
72	31382.5
73	22272.0
74	22272.0
75	16352.0
76	10432.0
77	10432.0
78	10324.5
79	10217.0
80	7745.5
81	5274.0
82	5274.0
83	5228.0
84	5182.0
85	5182.0
86	3296.0
87	1410.0
88	1171.0
89	932.0
90	932.0
91	557.5
92	183.0
93	136.5
94	90.0
95	90.0
96	73.5
97	57.0
98	57.0
99	49.5
100	42.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008373716449429221
2	0.0
3	0.0
4	1.4375478883140296E-4
5	3.593869720785074E-5
6	7.187739441570148E-5
7	5.390804581177611E-4
8	0.002695402290588805
9	0.003845440601240029
10	0.0010781609162355222
11	0.004636091939812745
12	3.593869720785074E-4
13	1.796934860392537E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	3.593869720785074E-5
22	1.0781609162355222E-4
23	7.187739441570148E-5
24	0.0
25	7.187739441570148E-5
26	1.4375478883140296E-4
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.0781609162355222E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2782516.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.80073037693869
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.21535755593973	29.350532295161813
2	16.129137652175938	13.484194684222725
3	5.8163316328851815	7.293807311072789
4	2.668741561852403	4.4622138589089015
5	1.43243682832997	2.9938452821509145
6	0.8676987625713555	2.176226521358914
7	0.5735181951793494	1.6781435610072337
8	0.3899665324664167	1.3040708703726713
9	0.2964103566619598	1.1151152459782934
>10	1.3910190954042752	10.241741496056704
>50	0.09384636879704193	2.749257881074264
>100	0.09626767997110243	8.840484867297677
>500	0.018010940163347613	5.244647601718708
>1k	0.010997064426657823	7.9820194187684725
>5k	1.7318211695524133E-4	0.578081443004622
>10k+	8.659105847762067E-5	0.5056176618453175
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	13969	0.5020276612964669	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	9798	0.3521273552425215	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	6173	0.2218495778640626	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4015	0.1442938692895207	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	4013	0.144221991895105	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	3669	0.13185908005560434	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	3659	0.13149969308352583	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	3469	0.1246713406140342	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	3262	0.1172320302920091	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	3209	0.11532727933999301	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	3164	0.11371003796563973	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	3031	0.10893019123699559	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2981	0.10713325637660305	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2962	0.10645042112965389	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2933	0.10540819891062621	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	2911	0.1046175475720535	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2872	0.10321593838094731	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.796934860392537E-4
2	0.0	0.0	0.0	3.593869720785074E-5	1.796934860392537E-4
3	0.0	0.0	0.0	7.187739441570148E-5	1.796934860392537E-4
4	0.0	0.0	0.0	7.187739441570148E-5	1.796934860392537E-4
5	0.0	0.0	0.0	7.187739441570148E-5	1.796934860392537E-4
6	0.0	0.0	0.0	7.187739441570148E-5	2.875095776628059E-4
7	0.0	0.0	0.0	7.187739441570148E-5	2.875095776628059E-4
8	0.0	0.0	0.0	1.0781609162355221E-4	3.234482748706566E-4
9	0.0	0.0	0.0	1.4375478883140296E-4	3.234482748706566E-4
10	3.593869720785074E-5	0.0	0.0	1.4375478883140296E-4	3.593869720785074E-4
11	3.593869720785074E-5	0.0	0.0	1.4375478883140296E-4	3.593869720785074E-4
12	3.593869720785074E-5	0.0	0.0	1.4375478883140296E-4	8.984674301962684E-4
13	3.593869720785074E-5	0.0	0.0	1.4375478883140296E-4	9.703448246119699E-4
14	3.593869720785074E-5	0.0	0.0	1.4375478883140296E-4	0.0010062835218198206
15	3.593869720785074E-5	0.0	0.0	1.4375478883140296E-4	0.0012937930994826265
16	3.593869720785074E-5	0.0	0.0	1.4375478883140296E-4	0.0012937930994826265
17	3.593869720785074E-5	0.0	0.0	1.4375478883140296E-4	0.001365670493898328
18	3.593869720785074E-5	0.0	0.0	2.1563218324710442E-4	0.0014375478883140295
19	3.593869720785074E-5	0.0	0.0	2.1563218324710442E-4	0.0014375478883140295
20	3.593869720785074E-5	0.0	0.0	3.234482748706566E-4	0.0015813026771454323
21	3.593869720785074E-5	0.0	0.0	3.593869720785074E-4	0.0017969348603925368
22	3.593869720785074E-5	0.0	0.0	6.468965497413132E-4	0.0017969348603925368
23	3.593869720785074E-5	0.0	0.0	0.0013297317966904773	0.001904750952016089
24	3.593869720785074E-5	0.0	0.0	0.0022641379240945963	0.0019766283464317906
25	3.593869720785074E-5	0.0	0.0	0.0027672796850045068	0.0019766283464317906
26	3.593869720785074E-5	0.0	0.0	0.00341417623474582	0.002156321832471044
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACCGT	55	0.0013657375	17.453722	25
ATCGTTT	570	0.0	17.402687	29
ACCGTCG	685	0.0	16.58614	8
CGTCGTA	685	0.0	16.350769	10
CTAGCGG	715	0.0	16.111128	29
TCTAGCG	720	0.0	15.999246	28
GTATAGG	255	0.0	15.687791	1
CCGTCGT	720	0.0	15.556779	9
TACCGTC	740	0.0	15.353661	7
CGTTTAT	655	0.0	15.144323	31
ATACCGT	780	0.0	14.976613	6
CGGCATC	675	0.0	14.932628	25
TAGCGGC	775	0.0	14.863813	30
CGGACGA	65	0.0041648615	14.768534	26
GTTTTCG	920	0.0	14.434101	28
CATCGTT	680	0.0	14.352264	28
GTCGTAG	770	0.0	14.337759	11
TCGTTTA	695	0.0	14.272708	30
TACCGTA	90	1.53346E-4	14.224364	7
GTCCTAT	1020	0.0	13.962133	1
>>END_MODULE