FastQCFastQC Report
Thu 2 Feb 2017
SRR4062958_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062958_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences973249
Sequences flagged as poor quality0
Sequence length38
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA78390.8054464993028506No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA73280.752941950107321No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG66180.6799904238278179No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC60670.6233759294897812No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA38080.3912667775666864No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA33080.3398924632853463No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA32290.33177532162889456No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA30850.31697951911586864No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG29030.2982792687174608No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA27650.2840999579758109No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT24830.25512484472113506No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT23860.2451582277505551No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAAT21850.22450575340945636No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA21510.22101230003832525No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAAT21470.22060130552407453No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT17930.1842282910128857No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT17470.1795018540990024No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCA16590.17045997478548655No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACG16350.16799400769998224No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAG16310.16758301318573152No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGT16070.16511704610022718No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAA15200.15617791541527398No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTT14840.1524789647870175No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTC14570.14970475181582513No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAA14300.14693053884463278No Hit
GTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAG14270.14662229295894474No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAA14130.1451838121590672No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA13690.14066287250230927No Hit
ACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAA13290.13655292735980207No Hit
CACTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAA13080.1343952061599858No Hit
CCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA12880.13234023358873218No Hit
ACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT12560.12905227747472642No Hit
GACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAA12450.12792204256053694No Hit
GAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTT12380.12720280216059818No Hit
CCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGT11890.12216811936102683No Hit
AGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCT11400.11713343656145549No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC10730.11024927844775591No Hit
ATACACACTTTAGGACGTGAAATATGGCGAGGAAAACT10610.10901629490500375No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT10030.1030568744483683No Hit
GTTCTACAGTGTGGTTTTTATCATTTTCCATGTTTCTC9890.10161839364849078No Hit
GAAATACACACTTTAGGACGTGAAATATGGCGAGGAAA9820.10089915324855202No Hit
ATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCAC9800.10069365599142666No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAG200.003760206531.9878735
AGAGTCG200.003760206531.9878735
TAGGACC18100.023.6804124
TCGCTAT350.002065927322.85787832
CCGTAGG453.5146432E-421.3526761
TAAGACT555.851461E-520.3548744
GACGTAG400.00448616119.9975597
GACCTAG801.2666351E-719.9975597
GCGTGGA507.190809E-419.20160520
TACGTCC507.213224E-419.1927245
AGGACCT35250.019.147355
GGACCTG34700.019.0360136
TAGAAAT17650.019.0286374
ATGTCCA17200.018.88539
GTCGTAC852.3723442E-718.8405971
GTCCTAT2300.018.799641
AAATGTC17650.018.7625837
GTATAGG601.1300715E-418.6835921
TGTCCAC17850.018.37688610
ATTTAGA17550.018.3414021
Produced by FastQC (version 0.11.5)