##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062958_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	973249
Sequences flagged as poor quality	0
Sequence length	38
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32209640081829	32.0	32.0	32.0	32.0	32.0
2	31.09053387159915	32.0	32.0	32.0	32.0	32.0
3	31.113476612870908	32.0	32.0	32.0	32.0	32.0
4	31.163092898117544	32.0	32.0	32.0	32.0	32.0
5	31.16969552498898	32.0	32.0	32.0	32.0	32.0
6	34.84135200755408	36.0	36.0	36.0	36.0	36.0
7	34.764018252266375	36.0	36.0	36.0	32.0	36.0
8	34.75364269575412	36.0	36.0	36.0	32.0	36.0
9	34.816076872413944	36.0	36.0	36.0	32.0	36.0
10	34.6419179470002	36.0	36.0	36.0	32.0	36.0
11	34.81345883735817	36.0	36.0	36.0	36.0	36.0
12	34.69329020630897	36.0	36.0	36.0	32.0	36.0
13	34.72751063705177	36.0	36.0	36.0	32.0	36.0
14	34.68522957639823	36.0	36.0	36.0	32.0	36.0
15	34.66274406652357	36.0	36.0	36.0	32.0	36.0
16	34.68069733439233	36.0	36.0	36.0	32.0	36.0
17	34.68275333444987	36.0	36.0	36.0	32.0	36.0
18	34.72053606014494	36.0	36.0	36.0	32.0	36.0
19	34.59212287913987	36.0	36.0	36.0	32.0	36.0
20	34.56143700121962	36.0	36.0	36.0	32.0	36.0
21	34.49173130411642	36.0	36.0	36.0	32.0	36.0
22	34.45551344003436	36.0	36.0	36.0	32.0	36.0
23	34.482294870069225	36.0	36.0	36.0	32.0	36.0
24	34.42127348705213	36.0	36.0	36.0	32.0	36.0
25	34.39854138046893	36.0	36.0	36.0	32.0	36.0
26	34.4167433000188	36.0	36.0	36.0	32.0	36.0
27	34.33710592047873	36.0	36.0	36.0	32.0	36.0
28	34.210369597091805	36.0	36.0	36.0	32.0	36.0
29	34.13053185772603	36.0	36.0	36.0	32.0	36.0
30	34.05770157482823	36.0	36.0	36.0	32.0	36.0
31	34.06091555192967	36.0	36.0	36.0	32.0	36.0
32	34.02408633350766	36.0	36.0	36.0	32.0	36.0
33	34.08931835532325	36.0	36.0	36.0	32.0	36.0
34	34.027492450544514	36.0	36.0	36.0	32.0	36.0
35	34.02398974979681	36.0	36.0	36.0	32.0	36.0
36	33.91774664037672	36.0	36.0	36.0	32.0	36.0
37	33.79701186438414	36.0	36.0	36.0	32.0	36.0
38	33.24517569501741	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	2.0
9	4.0
10	3.0
11	13.0
12	5.0
13	8.0
14	133.0
15	265.0
16	310.0
17	374.0
18	470.0
19	557.0
20	707.0
21	914.0
22	1325.0
23	2006.0
24	3004.0
25	4731.0
26	7233.0
27	10950.0
28	15929.0
29	22266.0
30	30657.0
31	41436.0
32	57083.0
33	86215.0
34	210762.0
35	475884.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.577743530760074	20.67224746739532	12.822227457580738	25.927781544263873
2	9.894889862587823	20.328181319099325	52.348426123734704	17.428502694578153
3	19.6524966781864	26.169417118053097	31.19565764715917	22.982428556601338
4	9.929655903725381	18.100092887909774	41.61316706671489	30.357084141649953
5	8.794590473528789	42.04620414571444	38.32217137291086	10.83703400784591
6	28.145373168373496	40.604697471453804	19.778843397410935	11.471085962761766
7	24.701284049611147	32.55251225534267	23.422166372634344	19.32403732241184
8	19.576673910474753	48.509779140915185	20.140252454417954	11.773294494192111
9	26.544032501512977	15.01324946288044	21.618253125876578	36.82446490973001
10	15.162708593845526	29.957472080456782	37.55197828291598	17.32784104278171
11	33.09337585848331	18.982293974279408	32.07357371551168	15.850756451725598
12	26.62097664979835	22.6832438541961	37.08094222815896	13.61483726784659
13	31.93737938008299	24.01217554655338	26.393847279513473	17.656597793850157
14	16.673551510966426	28.31150734931439	27.542254447403902	27.47268669231528
15	17.95117930418535	44.451890000811936	22.112582978841488	15.484347716161231
16	15.331408964653342	27.595816810240738	39.87841333096298	17.19436089414294
17	16.185162385010898	33.21819435637449	33.9874433083563	16.60919995025832
18	16.44689047428706	26.78210868003785	42.86791747115405	13.903083374521033
19	24.333164128247866	24.821358477072025	27.933019447624176	22.912457947055934
20	23.140331119295016	30.905152571353412	31.7573038603183	14.197212449033275
21	19.158662911315275	27.17241974580477	27.055484597544773	26.61343274533518
22	21.766191009053834	36.16847465882433	27.5949234416279	14.470410890493937
23	16.741629493530795	34.84728443434664	32.04493864762745	16.366147424495118
24	23.793605218753147	28.053058967882226	33.30983469426763	14.843501119096993
25	19.75810554030598	31.538667864252172	33.53903988309154	15.164186712350306
26	13.219107832517683	34.421805341165786	36.0418726350039	16.31721419131264
27	16.011858276131367	33.47661227341855	33.078322166961236	17.43320728348884
28	14.90856891548636	34.979736783697355	28.709853759587006	21.401840541229276
29	19.064447312605843	29.49001989444435	27.74062412653524	23.70490866641456
30	15.46556050365079	30.8603246562421	32.09859290967865	21.575521930428458
31	22.699075715305927	27.04922496750361	30.995648309417017	19.256051007773443
32	17.23142971348598	33.88701587885502	31.605117650262386	17.27643675739662
33	15.271716512616052	33.81019662647136	36.08997446476189	14.828112396150706
34	17.232864296511565	33.15254195486929	35.15577071487952	14.458823033739618
35	15.548076478416583	34.83693613610322	33.80794072460268	15.80704666087752
36	14.286007900589052	32.49805773760343	35.69785796204265	17.518076399764873
37	15.612678480596417	32.617338628878535	28.800949498790008	22.969033391735046
38	17.96761166884164	37.304020797583206	28.068825203711505	16.659542329863644
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	568.0
1	432.5
2	297.0
3	297.0
4	598.0
5	899.0
6	899.0
7	979.0
8	1059.0
9	1042.5
10	1026.0
11	1026.0
12	1319.5
13	1613.0
14	2079.0
15	2545.0
16	2545.0
17	3683.5
18	4822.0
19	4822.0
20	6106.0
21	7390.0
22	10309.0
23	13228.0
24	13228.0
25	21868.5
26	30509.0
27	30509.0
28	44056.5
29	57604.0
30	80791.5
31	103979.0
32	103979.0
33	128718.5
34	153458.0
35	153458.0
36	154544.5
37	155631.0
38	152194.5
39	148758.0
40	148758.0
41	123291.0
42	97824.0
43	77356.5
44	56889.0
45	56889.0
46	49607.0
47	42325.0
48	42325.0
49	36497.0
50	30669.0
51	25257.0
52	19845.0
53	19845.0
54	17524.0
55	15203.0
56	15203.0
57	12640.5
58	10078.0
59	8241.5
60	6405.0
61	6405.0
62	5252.0
63	4099.0
64	3289.0
65	2479.0
66	2479.0
67	2034.0
68	1589.0
69	1589.0
70	1224.5
71	860.0
72	677.5
73	495.0
74	495.0
75	392.0
76	289.0
77	289.0
78	232.5
79	176.0
80	141.0
81	106.0
82	106.0
83	90.0
84	74.0
85	74.0
86	51.0
87	28.0
88	29.0
89	30.0
90	30.0
91	21.5
92	13.0
93	14.0
94	15.0
95	15.0
96	22.0
97	29.0
98	29.0
99	186.0
100	343.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.125969818617846
2	0.057025488852287545
3	0.013973813484524514
4	0.0033907047425684486
5	3.0824588568804077E-4
6	2.054972571253605E-4
7	0.0
8	4.10994514250721E-4
9	2.054972571253605E-4
10	6.164917713760815E-4
11	0.0021577211998162855
12	0.002465967085504326
13	0.01551504291296472
14	0.009966616970579984
15	0.02671464342629687
16	0.01191884091327091
17	0.02281019554091502
18	0.006370414970886176
19	0.007295152627950299
20	0.006678660856574217
21	0.006164917713760816
22	0.007192403999387619
23	0.010274862856268026
24	0.014693053884463276
25	0.018494753141282447
26	0.020857971598224095
27	0.009144627942078544
28	0.005856671828072775
29	0.01161059502758287
30	0.0035962019996938093
31	0.006575912228011536
32	0.006986906742262257
33	0.008630884799265142
34	0.012329835427521632
35	0.016850775084279564
36	0.016131534684340802
37	0.010685857370518748
38	0.006062169085198136
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	973249.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.26293512276649
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.75386503214733	36.55156953862726
2	10.903114551055822	9.87013933121062
3	3.228136214075153	4.383447600754101
4	1.412112939812454	2.556655055230007
5	0.7737541780801324	1.7511192581705273
6	0.4989262401231291	1.3549719622643406
7	0.3530560654335281	1.118624764909189
8	0.26212796107945546	0.9491744716961962
9	0.2072702770798174	0.844349499390745
>10	1.307730197972588	11.537556964502503
>50	0.15826569926323178	5.03211316675967
>100	0.1204628627229335	10.899748812914964
>500	0.012296776154135627	3.7087325812878733
>1k	0.007970132692495314	6.571038839482644
>5k	9.108723077137501E-4	2.8707581527994512
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA	7839	0.8054464993028506	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	7328	0.752941950107321	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG	6618	0.6799904238278179	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	6067	0.6233759294897812	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	3808	0.3912667775666864	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA	3308	0.3398924632853463	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA	3229	0.33177532162889456	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	3085	0.31697951911586864	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG	2903	0.2982792687174608	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA	2765	0.2840999579758109	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2483	0.25512484472113506	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT	2386	0.2451582277505551	No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAAT	2185	0.22450575340945636	No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	2151	0.22101230003832525	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAAT	2147	0.22060130552407453	No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT	1793	0.1842282910128857	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1747	0.1795018540990024	No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCA	1659	0.17045997478548655	No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACG	1635	0.16799400769998224	No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAG	1631	0.16758301318573152	No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGT	1607	0.16511704610022718	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAA	1520	0.15617791541527398	No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTT	1484	0.1524789647870175	No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTC	1457	0.14970475181582513	No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAA	1430	0.14693053884463278	No Hit
GTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAG	1427	0.14662229295894474	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAA	1413	0.1451838121590672	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1369	0.14066287250230927	No Hit
ACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAA	1329	0.13655292735980207	No Hit
CACTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAA	1308	0.1343952061599858	No Hit
CCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	1288	0.13234023358873218	No Hit
ACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT	1256	0.12905227747472642	No Hit
GACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAA	1245	0.12792204256053694	No Hit
GAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTT	1238	0.12720280216059818	No Hit
CCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGT	1189	0.12216811936102683	No Hit
AGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCT	1140	0.11713343656145549	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1073	0.11024927844775591	No Hit
ATACACACTTTAGGACGTGAAATATGGCGAGGAAAACT	1061	0.10901629490500375	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1003	0.1030568744483683	No Hit
GTTCTACAGTGTGGTTTTTATCATTTTCCATGTTTCTC	989	0.10161839364849078	No Hit
GAAATACACACTTTAGGACGTGAAATATGGCGAGGAAA	982	0.10089915324855202	No Hit
ATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCAC	980	0.10069365599142666	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.0274862856268026E-4	0.0	0.0	0.0
6	0.0	1.0274862856268026E-4	0.0	0.0	0.0
7	0.0	1.0274862856268026E-4	0.0	0.0	0.0
8	0.0	1.0274862856268026E-4	0.0	1.0274862856268026E-4	0.0
9	0.0	1.0274862856268026E-4	0.0	1.0274862856268026E-4	0.0
10	0.0	1.0274862856268026E-4	0.0	1.0274862856268026E-4	0.0
11	0.0	1.0274862856268026E-4	0.0	1.0274862856268026E-4	0.0
12	0.0	1.0274862856268026E-4	0.0	1.0274862856268026E-4	0.0
13	0.0	1.0274862856268026E-4	0.0	1.0274862856268026E-4	0.0
14	0.0	1.0274862856268026E-4	0.0	1.0274862856268026E-4	0.0
15	0.0	1.0274862856268026E-4	0.0	2.0549725712536051E-4	0.0
16	0.0	1.0274862856268026E-4	0.0	2.0549725712536051E-4	0.0
17	0.0	1.0274862856268026E-4	0.0	3.0824588568804077E-4	0.0
18	0.0	1.0274862856268026E-4	0.0	3.0824588568804077E-4	0.0
19	0.0	1.0274862856268026E-4	0.0	3.0824588568804077E-4	0.0
20	0.0	1.0274862856268026E-4	0.0	6.164917713760815E-4	0.0
21	0.0	1.0274862856268026E-4	0.0	7.192403999387618E-4	0.0
22	0.0	1.0274862856268026E-4	1.0274862856268026E-4	0.0013357321713148435	0.0
23	0.0	1.0274862856268026E-4	1.0274862856268026E-4	0.001952223942690925	0.0
24	0.0	1.0274862856268026E-4	1.0274862856268026E-4	0.002774212971192367	0.0
25	0.0	1.0274862856268026E-4	1.0274862856268026E-4	0.003596201999693809	0.0
26	0.0	1.0274862856268026E-4	1.0274862856268026E-4	0.0056511745709474145	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGAG	20	0.0037602065	31.987873	5
AGAGTCG	20	0.0037602065	31.987873	5
TAGGACC	1810	0.0	23.680412	4
TCGCTAT	35	0.0020659273	22.857878	32
CCGTAGG	45	3.5146432E-4	21.352676	1
TAAGACT	55	5.851461E-5	20.354874	4
GACGTAG	40	0.004486161	19.997559	7
GACCTAG	80	1.2666351E-7	19.997559	7
GCGTGGA	50	7.190809E-4	19.201605	20
TACGTCC	50	7.213224E-4	19.192724	5
AGGACCT	3525	0.0	19.14735	5
GGACCTG	3470	0.0	19.036013	6
TAGAAAT	1765	0.0	19.028637	4
ATGTCCA	1720	0.0	18.8853	9
GTCGTAC	85	2.3723442E-7	18.840597	1
GTCCTAT	230	0.0	18.79964	1
AAATGTC	1765	0.0	18.762583	7
GTATAGG	60	1.1300715E-4	18.683592	1
TGTCCAC	1785	0.0	18.376886	10
ATTTAGA	1755	0.0	18.341402	1
>>END_MODULE