FastQCFastQC Report
Thu 2 Feb 2017
SRR4062958_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062958_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences973249
Sequences flagged as poor quality0
Sequence length38
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA77970.801131056903218No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA71460.7342416997089131No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG70360.7229393505670183No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC56530.5808379972648315No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA41430.4256875681351843No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA35950.36938131968283555No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA32200.3308505839718304No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA31070.31923998894424754No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG30050.30875962883085417No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT29280.30084798443152777No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT26380.27105088214835055No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA25900.26611894797734187No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA22610.23231464918022007No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT22400.2301569279804038No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAAT21440.22029305963838647No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAAT19980.20529175986823517No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT19450.1998460825544131No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAG18360.18864648204108098No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAA17290.17765237878487417No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCA16710.17169295832823872No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACG16590.17045997478548655No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAA15720.16152084410053338No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTT15250.1566916585580874No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGT15020.15432844010114577No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTC14940.15350645107264432No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAA14880.15288995930126822No Hit
ACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT14630.15032124358720123No Hit
ACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAA14620.15021849495863854No Hit
CACTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAA14230.14621129844469402No Hit
CCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA13960.14343708547350165No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT13840.14220410193074948No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC13700.14076562113087196No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA13670.14045737524518392No Hit
GTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAG13370.13737491638830351No Hit
GACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAA13270.1363474301026767No Hit
GAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTT12490.12833303707478766No Hit
ATACACACTTTAGGACGTGAAATATGGCGAGGAAAACT12420.1276137966748489No Hit
CCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGT12310.12648356176065942No Hit
GAAATACACACTTTAGGACGTGAAATATGGCGAGGAAA11760.120832387189712No Hit
AGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCT11700.12021589541833591No Hit
ATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCAC10400.10685857370518748No Hit
CTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGT10080.10357061759118169No Hit
GTTCTACAGTGTGGTTTTTATCATTTTCCATGTTTCTC9800.10069365599142666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAC2500.022.4000723
CTCTAGG2700.021.9271241
TAGGACC20450.021.750684
GCGAGAG604.7213034E-621.33230620
CTACCGT555.8319674E-520.3637034
GTGTAGG1500.019.2010481
GTCCTAT1850.019.0280651
CCTGGTA601.13658156E-418.6696059
CGAGAGA601.1383745E-418.66576821
ATTTAGA18800.018.5542031
TCCTCGA953.7896825E-818.52542329
CCTCGAC953.7896825E-818.52542330
GGACGTT1055.9626473E-918.2885916
CGGTAGG701.7859616E-518.2867131
ATGTCCA19700.018.2769899
TTTAGAA19800.018.2617452
AGGACCT39950.018.1455355
TAGAAAT19600.018.1225074
AAATGTC19700.018.1145277
GGACCTG38750.018.0054136
Produced by FastQC (version 0.11.5)