##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062958_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	973249
Sequences flagged as poor quality	0
Sequence length	38
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24895581706223	32.0	32.0	32.0	32.0	32.0
2	31.437010467002793	32.0	32.0	32.0	32.0	32.0
3	31.489181083155493	32.0	32.0	32.0	32.0	32.0
4	31.56774473952709	32.0	32.0	32.0	32.0	32.0
5	31.540446483890555	32.0	32.0	32.0	32.0	32.0
6	35.07174217492132	36.0	36.0	36.0	36.0	36.0
7	35.10897416796729	36.0	36.0	36.0	36.0	36.0
8	35.033967669116535	36.0	36.0	36.0	36.0	36.0
9	35.13068906312773	36.0	36.0	36.0	36.0	36.0
10	35.041523803261036	36.0	36.0	36.0	36.0	36.0
11	35.144348465808854	36.0	36.0	36.0	36.0	36.0
12	35.09556136199472	36.0	36.0	36.0	36.0	36.0
13	35.12102349963884	36.0	36.0	36.0	36.0	36.0
14	35.08224102978785	36.0	36.0	36.0	36.0	36.0
15	35.05553460625184	36.0	36.0	36.0	36.0	36.0
16	35.09751358593741	36.0	36.0	36.0	36.0	36.0
17	35.110745554323714	36.0	36.0	36.0	36.0	36.0
18	35.10283596489696	36.0	36.0	36.0	36.0	36.0
19	35.04219166934669	36.0	36.0	36.0	36.0	36.0
20	35.021358357419324	36.0	36.0	36.0	36.0	36.0
21	34.96957613108259	36.0	36.0	36.0	36.0	36.0
22	34.952700696327454	36.0	36.0	36.0	36.0	36.0
23	34.94528327283152	36.0	36.0	36.0	36.0	36.0
24	34.91785349895042	36.0	36.0	36.0	36.0	36.0
25	34.88819818977466	36.0	36.0	36.0	36.0	36.0
26	34.83740029529956	36.0	36.0	36.0	32.0	36.0
27	34.751308246913176	36.0	36.0	36.0	32.0	36.0
28	34.66530250737478	36.0	36.0	36.0	32.0	36.0
29	34.63166568884222	36.0	36.0	36.0	32.0	36.0
30	34.55678762577716	36.0	36.0	36.0	32.0	36.0
31	34.54901160956754	36.0	36.0	36.0	32.0	36.0
32	34.51024455201084	36.0	36.0	36.0	32.0	36.0
33	34.562815887814935	36.0	36.0	36.0	32.0	36.0
34	34.51115593234619	36.0	36.0	36.0	32.0	36.0
35	34.49317594983401	36.0	36.0	36.0	32.0	36.0
36	34.40096316564415	36.0	36.0	36.0	32.0	36.0
37	34.291733153591736	36.0	36.0	36.0	32.0	36.0
38	33.933784673809065	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	4.0
21	16.0
22	36.0
23	153.0
24	404.0
25	1142.0
26	2575.0
27	5192.0
28	9910.0
29	16320.0
30	25056.0
31	36346.0
32	51321.0
33	80796.0
34	207547.0
35	536431.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.270706746859936	21.856262504508557	13.29929949062271	26.57373125800879
2	9.562506614442965	21.253964812704663	51.16655655438639	18.016972018465985
3	19.318797142355145	26.781121788976918	31.56530343211244	22.334777636555497
4	9.883185084187089	18.9943169733542	41.71676518547668	29.40573275698203
5	8.594717282011079	42.64725676574032	37.72919365958763	11.028832292660974
6	28.525720063128823	40.69507463668048	19.321899454198725	11.457305845991977
7	24.724633392516605	32.75424146774512	23.470687841125798	19.05043729861248
8	19.712377764146066	48.67455593333888	19.878527076583836	11.734539225931211
9	25.702580564283938	14.964375183672798	21.791146303218873	37.54189794882439
10	15.177716401777165	30.428077054280774	36.92557611925576	17.468630424686303
11	32.69995694602429	19.023178246542066	32.33836520252323	15.938499604910414
12	26.573706079873084	22.99433646649761	36.74094060687762	13.691016846751689
13	31.805968692364207	23.98923189947033	26.490863040652663	17.713936367512805
14	16.734258139489484	28.605218191850184	27.436863536463946	27.223660132196386
15	18.018924242408673	44.47207240901352	22.010400216183115	15.49860313239469
16	15.497678394737626	27.904369796424145	39.1397268324961	17.45822497634213
17	16.321825144438883	33.771932979124564	33.169106775347316	16.737135101089237
18	16.65781315983885	26.958260424618985	42.470323627355384	13.913602788186786
19	24.67569964109904	24.968224986616992	28.2318296756534	22.124245696630567
20	23.514229143826505	31.293327812307027	31.24554970002538	13.946893343841094
21	19.331044091536793	27.01171746564082	27.36517311106727	26.29206533175511
22	22.141221970145327	35.592367002038536	27.779969750772672	14.486441277043467
23	16.708074406523313	34.90549171434208	31.742782928914316	16.643650950220295
24	24.08109332760681	28.32060449073156	32.6617340475048	14.93656813415683
25	20.0256461103913	31.834673006955068	32.8133557051807	15.326325177472933
26	13.437362894798763	34.616937700424046	35.49317800480658	16.452521399970614
27	16.292952779812772	33.74799254866946	32.578507658369034	17.380547013148743
28	15.043426707862018	34.94244535571062	29.01097252604421	21.003155410383158
29	19.085146761003607	29.21318182705556	27.999001283330372	23.70267012861046
30	15.688379849349962	30.5662785165975	32.15631354360497	21.589028090447563
31	22.37176714283806	27.06892069758099	31.27277808659449	19.286534072986463
32	17.317767601096946	33.732272008499365	31.53160188194388	17.418358508459807
33	15.409931065945099	34.00476137144759	35.52256411257551	15.062743450031801
34	17.686635177636965	33.18883451203135	34.528162885345886	14.596367424985795
35	15.921516487558682	35.0148831388473	33.01179862501785	16.05180174857616
36	14.396110347917132	32.50134343831846	35.34614471733338	17.756401496431025
37	15.76852377962885	32.489013602890935	28.665120642302227	23.077341975177987
38	18.233706345871088	36.81788898398865	28.346555396523183	16.601849273617077
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	284.0
1	205.0
2	126.0
3	126.0
4	489.5
5	853.0
6	853.0
7	999.0
8	1145.0
9	1140.5
10	1136.0
11	1136.0
12	1416.0
13	1696.0
14	2176.0
15	2656.0
16	2656.0
17	3932.5
18	5209.0
19	5209.0
20	6392.0
21	7575.0
22	10153.5
23	12732.0
24	12732.0
25	20957.0
26	29182.0
27	29182.0
28	42767.5
29	56353.0
30	79369.0
31	102385.0
32	102385.0
33	126484.0
34	150583.0
35	150583.0
36	153225.5
37	155868.0
38	154453.5
39	153039.0
40	153039.0
41	126518.0
42	99997.0
43	78862.5
44	57728.0
45	57728.0
46	50213.0
47	42698.0
48	42698.0
49	36819.5
50	30941.0
51	25549.5
52	20158.0
53	20158.0
54	17496.0
55	14834.0
56	14834.0
57	12315.0
58	9796.0
59	8126.0
60	6456.0
61	6456.0
62	5257.0
63	4058.0
64	3204.5
65	2351.0
66	2351.0
67	1917.5
68	1484.0
69	1484.0
70	1176.0
71	868.0
72	664.0
73	460.0
74	460.0
75	361.0
76	262.0
77	262.0
78	213.0
79	164.0
80	130.5
81	97.0
82	97.0
83	71.0
84	45.0
85	45.0
86	30.5
87	16.0
88	11.0
89	6.0
90	6.0
91	4.0
92	2.0
93	2.0
94	2.0
95	2.0
96	2.5
97	3.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010274862856268026
2	0.0
3	0.0
4	0.0
5	0.0
6	1.0274862856268024E-4
7	1.0274862856268024E-4
8	0.002876961599755047
9	0.005240180056696694
10	0.0013357321713148435
11	0.005342928685259374
12	5.137431428134013E-4
13	4.10994514250721E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.0274862856268024E-4
22	1.0274862856268024E-4
23	1.0274862856268024E-4
24	0.0
25	2.054972571253605E-4
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	2.054972571253605E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	973249.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.53489290403475
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.64894791161355	34.675084172511376
2	11.615965721278721	10.113996473056158
3	3.3058951491089013	4.317653738052721
4	1.4555574008159418	2.5347014224078883
5	0.8410450463177751	1.830740300945664
6	0.5449057371112187	1.4233447744758612
7	0.3769082632498192	1.148606261265863
8	0.2866507943991133	0.9983449308017506
9	0.22052523503496238	0.8640488240895762
>10	1.3796983503151712	11.638152513659394
>50	0.1723838454858114	5.288491600319237
>100	0.12875349621663706	11.262153238217065
>500	0.012804215091951222	3.729826954900438
>1k	0.009010373583224932	7.3224641604633725
>5k	9.484603771815719E-4	2.852390634833635
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	7797	0.801131056903218	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA	7146	0.7342416997089131	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG	7036	0.7229393505670183	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	5653	0.5808379972648315	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	4143	0.4256875681351843	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA	3595	0.36938131968283555	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	3220	0.3308505839718304	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA	3107	0.31923998894424754	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG	3005	0.30875962883085417	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2928	0.30084798443152777	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT	2638	0.27105088214835055	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA	2590	0.26611894797734187	No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	2261	0.23231464918022007	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2240	0.2301569279804038	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAAT	2144	0.22029305963838647	No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAAT	1998	0.20529175986823517	No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT	1945	0.1998460825544131	No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAG	1836	0.18864648204108098	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAA	1729	0.17765237878487417	No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCA	1671	0.17169295832823872	No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACG	1659	0.17045997478548655	No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAA	1572	0.16152084410053338	No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTT	1525	0.1566916585580874	No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGT	1502	0.15432844010114577	No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTC	1494	0.15350645107264432	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAA	1488	0.15288995930126822	No Hit
ACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT	1463	0.15032124358720123	No Hit
ACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAA	1462	0.15021849495863854	No Hit
CACTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAA	1423	0.14621129844469402	No Hit
CCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	1396	0.14343708547350165	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1384	0.14220410193074948	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1370	0.14076562113087196	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1367	0.14045737524518392	No Hit
GTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAG	1337	0.13737491638830351	No Hit
GACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAA	1327	0.1363474301026767	No Hit
GAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTT	1249	0.12833303707478766	No Hit
ATACACACTTTAGGACGTGAAATATGGCGAGGAAAACT	1242	0.1276137966748489	No Hit
CCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGT	1231	0.12648356176065942	No Hit
GAAATACACACTTTAGGACGTGAAATATGGCGAGGAAA	1176	0.120832387189712	No Hit
AGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCT	1170	0.12021589541833591	No Hit
ATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCAC	1040	0.10685857370518748	No Hit
CTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGT	1008	0.10357061759118169	No Hit
GTTCTACAGTGTGGTTTTTATCATTTTCCATGTTTCTC	980	0.10069365599142666	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	2.0549725712536051E-4	0.0	0.0	0.0
15	0.0	2.0549725712536051E-4	0.0	0.0	0.0
16	0.0	2.0549725712536051E-4	0.0	0.0	0.0
17	0.0	2.0549725712536051E-4	0.0	1.0274862856268026E-4	0.0
18	0.0	2.0549725712536051E-4	0.0	2.0549725712536051E-4	0.0
19	0.0	2.0549725712536051E-4	0.0	2.0549725712536051E-4	0.0
20	0.0	2.0549725712536051E-4	0.0	6.164917713760815E-4	0.0
21	0.0	2.0549725712536051E-4	0.0	8.219890285014421E-4	0.0
22	0.0	2.0549725712536051E-4	0.0	0.0016439780570028841	0.0
23	0.0	2.0549725712536051E-4	0.0	0.002363218456941646	0.0
24	0.0	2.0549725712536051E-4	0.0	0.0033907047425684486	0.0
25	0.0	2.0549725712536051E-4	0.0	0.00390444788538185	0.0
26	0.0	2.0549725712536051E-4	0.0	0.005548425942384734	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGGAC	250	0.0	22.400072	3
CTCTAGG	270	0.0	21.927124	1
TAGGACC	2045	0.0	21.75068	4
GCGAGAG	60	4.7213034E-6	21.332306	20
CTACCGT	55	5.8319674E-5	20.363703	4
GTGTAGG	150	0.0	19.201048	1
GTCCTAT	185	0.0	19.028065	1
CCTGGTA	60	1.13658156E-4	18.669605	9
CGAGAGA	60	1.1383745E-4	18.665768	21
ATTTAGA	1880	0.0	18.554203	1
TCCTCGA	95	3.7896825E-8	18.525423	29
CCTCGAC	95	3.7896825E-8	18.525423	30
GGACGTT	105	5.9626473E-9	18.288591	6
CGGTAGG	70	1.7859616E-5	18.286713	1
ATGTCCA	1970	0.0	18.276989	9
TTTAGAA	1980	0.0	18.261745	2
AGGACCT	3995	0.0	18.145535	5
TAGAAAT	1960	0.0	18.122507	4
AAATGTC	1970	0.0	18.114527	7
GGACCTG	3875	0.0	18.005413	6
>>END_MODULE