FastQCFastQC Report
Thu 2 Feb 2017
SRR4062957_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062957_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1483703
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT29460.19855725842705718No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT22120.14908644115432806No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC20510.1382352128424624No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT19420.13088872907852853No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA19290.13001254294154557No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG19000.12805797386673748No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG18270.1231378517129102No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC18160.12239646344315541No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC17820.12010489970027693No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT17440.1175437402229422No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA16840.11349980420609786No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG16760.11296061273718527No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG16720.11269101700272899No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG15350.10345736309760106No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC15290.10305296949591662No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG15120.10190718762447741No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA15020.1012331982883367No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC14890.10035701215135373No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTAAA308.4079267E-426.6721462
CTAGCGG4200.021.333429
TCTAGCG4250.021.08099728
TAGCGGC4350.020.23063330
AGCGGCG4600.019.47897731
TTAGGAC7600.017.8887623
TGTAGGA13100.016.4919362
TAGGACA3600.016.43844
TAGGACG13550.016.2892134
TCCTACA13600.016.238632
GTCCTAC13250.016.191011
GTAGGAC12850.016.1813683
GTCCTAA6950.016.1249431
CGCGAAC600.002440106116.00220712
CTAGCAC1407.4578566E-1015.99465753
AGGACGT14000.015.881485
AAGACGG4800.015.6624925
TATACAC4950.015.5099733
CAAGACG4850.015.4994564
CTGTAGG13200.015.4033361
Produced by FastQC (version 0.11.5)