FastQCFastQC Report
Thu 2 Feb 2017
SRR4062957_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062957_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1483703
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT29640.19977043923211046No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT22190.14955823368962656No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT22020.14841245181818732No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC21860.14733406888036218No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG20110.1355392554978995No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA18990.1279905749331234No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC18800.12670999519445603No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC18490.12462062825241979No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG18260.12307045277929612No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG17440.1175437402229422No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC16920.11403899567501044No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT16880.11376939994055413No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG16650.11221922446743049No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA16430.11073644792792088No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA16040.10810788951697206No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG15210.10251377802700407No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG4000.020.798428
TATACCG555.8245925E-520.3682485
TAGCGGC4250.020.32784830
CTAGCGG4200.020.18892529
ATTTAGA4250.018.4493711
TAGCCCG550.00136282917.45855
CGCACGA550.001365602217.45320528
AGCGGCG4900.017.3047931
AACCGCG3150.017.2737527
GTCACGC652.1021569E-417.22944524
AGAACCG4000.017.2038975
TATACTG1404.0017767E-1117.146745
GTCCTAC11300.017.134891
TCGTTTA3400.016.93987530
GATATAC3500.016.9164071
CAAGACG6150.016.6519324
TGTAGGA14150.016.2825322
ATCGTTT3550.016.22410429
AAGACGG6450.016.1276825
TCACGCA600.00244360915.99876925
Produced by FastQC (version 0.11.5)