##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062957_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1483703
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22946843135048	32.0	32.0	32.0	32.0	32.0
2	31.31256390261393	32.0	32.0	32.0	32.0	32.0
3	31.387033658353456	32.0	32.0	32.0	32.0	32.0
4	31.48413395403258	32.0	32.0	32.0	32.0	32.0
5	31.408978077148863	32.0	32.0	32.0	32.0	32.0
6	34.94405955908966	36.0	36.0	36.0	36.0	36.0
7	34.96868510746423	36.0	36.0	36.0	36.0	36.0
8	34.912719728948446	36.0	36.0	36.0	36.0	36.0
9	35.01247823856931	36.0	36.0	36.0	36.0	36.0
10	34.86250347946995	36.0	36.0	36.0	32.0	36.0
11	35.031511023432586	36.0	36.0	36.0	36.0	36.0
12	34.931059652774174	36.0	36.0	36.0	32.0	36.0
13	34.980384888350294	36.0	36.0	36.0	36.0	36.0
14	34.936399670284416	36.0	36.0	36.0	32.0	36.0
15	34.904286774374654	36.0	36.0	36.0	32.0	36.0
16	34.92150180999836	36.0	36.0	36.0	32.0	36.0
17	34.881401466465995	36.0	36.0	36.0	32.0	36.0
18	34.88691267726762	36.0	36.0	36.0	32.0	36.0
19	34.87622118442842	36.0	36.0	36.0	32.0	36.0
20	34.861563938335365	36.0	36.0	36.0	32.0	36.0
21	34.8555222979262	36.0	36.0	36.0	32.0	36.0
22	34.83739468074136	36.0	36.0	36.0	32.0	36.0
23	34.77734155690189	36.0	36.0	36.0	32.0	36.0
24	34.75787202694879	36.0	36.0	36.0	32.0	36.0
25	34.73250172035778	36.0	36.0	36.0	32.0	36.0
26	34.672747847783555	36.0	36.0	36.0	32.0	36.0
27	34.64966977892476	36.0	36.0	36.0	32.0	36.0
28	34.61192098418619	36.0	36.0	36.0	32.0	36.0
29	34.58728465198224	36.0	36.0	36.0	32.0	36.0
30	34.55128890350697	36.0	36.0	36.0	32.0	36.0
31	34.55837522738715	36.0	36.0	36.0	32.0	36.0
32	34.53276363261381	36.0	36.0	36.0	32.0	36.0
33	34.50066623845878	36.0	36.0	36.0	32.0	36.0
34	34.5008232779741	36.0	36.0	36.0	32.0	36.0
35	34.4716954808341	36.0	36.0	36.0	32.0	36.0
36	34.43172589123295	36.0	36.0	36.0	32.0	36.0
37	34.42310421964504	36.0	36.0	36.0	32.0	36.0
38	33.98661322380557	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	8.0
21	20.0
22	101.0
23	317.0
24	877.0
25	2290.0
26	4738.0
27	9475.0
28	16492.0
29	26831.0
30	40698.0
31	58940.0
32	84858.0
33	135860.0
34	334690.0
35	767508.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.617487664806276	18.541683518024215	11.989538677235839	26.851290139933674
2	15.479647881011227	20.196157856390396	37.78215721070861	26.542037051889768
3	18.166708566337064	23.97319409612301	28.936788562131373	28.923308775408557
4	11.962752502701022	15.815493325119617	36.121323963046365	36.100430209132995
5	13.904678971922934	37.2464955900848	33.49783177484427	15.35099366314799
6	34.776525205196194	35.98896543915153	16.0893494785341	13.145159877118182
7	30.242852983360475	30.36430643474135	20.663869148396977	18.7289714335012
8	27.728875019377625	34.35797717821348	18.904877769315277	19.008270033093613
9	27.425800298584925	13.529334452184285	18.430980039294585	40.61388520993621
10	15.957532850572356	26.638282442130688	31.570249270736166	25.833935436560786
11	38.34914810526046	20.24151509064904	22.516200087083472	18.893136717007025
12	24.999292308812173	23.245094352085125	28.99390576788538	22.761707571217325
13	30.102001614879537	19.08732346522415	25.08374323145753	25.726931688438782
14	23.033129294157455	20.20951646624457	24.60419949558604	32.153154744011935
15	24.787575410981848	28.857527416201222	21.54049698625668	24.81440018656025
16	25.367678032598167	25.434133381141642	24.81143463348123	24.386753952778957
17	23.88314912081461	26.454553236058697	25.26846680231825	24.393830840808437
18	24.87168928013221	24.379609665815867	27.223440270728037	23.525260783323887
19	26.01389900808989	24.41095016994641	24.972787680553317	24.60236314141038
20	26.130431764308625	24.29704597213863	24.890560981544148	24.681961282008597
21	26.935983818864017	23.933496124224323	24.023608498466338	25.106911558445322
22	26.091223170151416	24.92643401437755	24.710487685532538	24.27185512993849
23	23.90912993925326	24.4878179633228	25.87799024196924	25.725061855454705
24	25.3587139744275	24.75515652391348	25.42119278588774	24.464936715771284
25	24.809429386763647	24.710285488595417	25.56915067850552	24.91113444613541
26	23.939157634647902	26.057640916005425	26.104820169535277	23.89838127981139
27	25.134073690148746	24.915498359168538	25.095319198840194	24.855108751842522
28	23.924262470319192	25.269612584189694	25.653921303657135	25.15220364183398
29	24.39106748453026	24.640578336769554	25.457049018570427	25.511305160129755
30	23.80786451196769	25.20854915033534	26.08615066492418	24.897435672772787
31	25.512113947333127	24.50578046954141	24.68735319669772	25.294752386427742
32	24.730825508878798	25.227016458145602	24.683713654282563	25.35844437869304
33	23.970902532380133	24.80334676144754	25.95222898383302	25.27352172233931
34	25.053329406222137	24.944008335900108	25.847423642063134	24.15523861581462
35	25.358376979759427	24.866903955845608	25.803142542678692	23.971576521716273
36	23.901818625425708	25.40515183968759	25.678926308027954	25.014103226858747
37	25.21811979890854	25.08433291568461	24.70480952050377	24.992737764903083
38	24.49966367909459	25.2594523698155	25.203713414149203	25.037170536940707
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	15.0
1	34.0
2	53.0
3	53.0
4	199.0
5	345.0
6	345.0
7	514.0
8	683.0
9	728.5
10	774.0
11	774.0
12	1051.5
13	1329.0
14	1892.0
15	2455.0
16	2455.0
17	3873.0
18	5291.0
19	5291.0
20	6309.0
21	7327.0
22	8563.5
23	9800.0
24	9800.0
25	12614.5
26	15429.0
27	15429.0
28	20451.0
29	25473.0
30	33320.5
31	41168.0
32	41168.0
33	53153.5
34	65139.0
35	65139.0
36	70830.5
37	76522.0
38	85251.0
39	93980.0
40	93980.0
41	93869.5
42	93759.0
43	102469.0
44	111179.0
45	111179.0
46	111592.0
47	112005.0
48	112005.0
49	119298.5
50	126592.0
51	128672.5
52	130753.0
53	130753.0
54	124801.0
55	118849.0
56	118849.0
57	116453.0
58	114057.0
59	102402.5
60	90748.0
61	90748.0
62	87565.0
63	84382.0
64	70386.0
65	56390.0
66	56390.0
67	47751.5
68	39113.0
69	39113.0
70	31583.0
71	24053.0
72	18978.0
73	13903.0
74	13903.0
75	10530.5
76	7158.0
77	7158.0
78	6824.5
79	6491.0
80	5125.0
81	3759.0
82	3759.0
83	3413.0
84	3067.0
85	3067.0
86	1989.0
87	911.0
88	748.0
89	585.0
90	585.0
91	341.5
92	98.0
93	67.0
94	36.0
95	36.0
96	25.5
97	15.0
98	15.0
99	16.0
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009638047506812346
2	0.0
3	0.0
4	4.0439360168443417E-4
5	6.739893361407236E-5
6	6.739893361407236E-5
7	4.7179253529850654E-4
8	0.002224164809264388
9	0.0025611594773347493
10	0.0010109840042110854
11	0.004650526419370993
12	3.369946680703618E-4
13	6.739893361407236E-5
14	6.739893361407236E-5
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	6.739893361407236E-5
23	1.3479786722814471E-4
24	0.0
25	6.739893361407236E-5
26	0.0
27	2.6959573445628943E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	6.739893361407236E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1483703.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.48303662158321
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.89724504269316	41.4076700091769
2	12.939595580815128	13.582185374727935
3	3.807254265409412	5.994487951174833
4	1.5083993656200316	3.166615165832361
5	0.7501033422324226	1.96838505901781
6	0.4434261511876496	1.3963410559049467
7	0.29951220946948054	1.1003517180738636
8	0.22067218052431847	0.9265236905457943
9	0.14500049260771627	0.6849059547310542
>10	0.8035426365634001	7.6610416725822095
>50	0.0784994444907952	2.90272925352854
>100	0.08803273077020192	10.017737947640342
>500	0.012778891062163294	4.618579729096122
>1k	0.00593766655413648	4.57244541796736
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2964	0.19977043923211046	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2219	0.14955823368962656	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2202	0.14841245181818732	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2186	0.14733406888036218	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2011	0.1355392554978995	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1899	0.1279905749331234	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1880	0.12670999519445603	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1849	0.12462062825241979	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1826	0.12307045277929612	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1744	0.1175437402229422	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1692	0.11403899567501044	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1688	0.11376939994055413	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1665	0.11221922446743049	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	1643	0.11073644792792088	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1604	0.10810788951697206	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1521	0.10251377802700407	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.739893361407236E-5
2	0.0	0.0	0.0	0.0	6.739893361407236E-5
3	0.0	0.0	0.0	0.0	6.739893361407236E-5
4	0.0	0.0	0.0	0.0	6.739893361407236E-5
5	0.0	0.0	0.0	0.0	6.739893361407236E-5
6	0.0	0.0	0.0	0.0	6.739893361407236E-5
7	0.0	0.0	0.0	0.0	6.739893361407236E-5
8	0.0	0.0	0.0	0.0	6.739893361407236E-5
9	0.0	0.0	0.0	0.0	6.739893361407236E-5
10	0.0	0.0	0.0	0.0	6.739893361407236E-5
11	0.0	0.0	0.0	0.0	6.739893361407236E-5
12	0.0	0.0	0.0	0.0	1.3479786722814471E-4
13	0.0	0.0	0.0	0.0	2.0219680084221709E-4
14	0.0	0.0	0.0	0.0	2.0219680084221709E-4
15	0.0	0.0	0.0	0.0	2.6959573445628943E-4
16	0.0	0.0	0.0	1.3479786722814471E-4	2.6959573445628943E-4
17	0.0	0.0	0.0	1.3479786722814471E-4	2.6959573445628943E-4
18	0.0	0.0	0.0	2.0219680084221709E-4	2.6959573445628943E-4
19	0.0	0.0	0.0	2.0219680084221709E-4	2.6959573445628943E-4
20	0.0	0.0	0.0	2.0219680084221709E-4	2.6959573445628943E-4
21	0.0	0.0	0.0	2.0219680084221709E-4	2.6959573445628943E-4
22	0.0	0.0	0.0	2.0219680084221709E-4	4.0439360168443417E-4
23	0.0	0.0	0.0	4.0439360168443417E-4	4.0439360168443417E-4
24	0.0	0.0	0.0	9.43585070597013E-4	4.0439360168443417E-4
25	0.0	0.0	0.0	0.0010783829378251577	4.0439360168443417E-4
26	0.0	0.0	0.0	0.0012131808050533024	4.717925352985065E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	400	0.0	20.7984	28
TATACCG	55	5.8245925E-5	20.368248	5
TAGCGGC	425	0.0	20.327848	30
CTAGCGG	420	0.0	20.188925	29
ATTTAGA	425	0.0	18.449371	1
TAGCCCG	55	0.001362829	17.4585	5
CGCACGA	55	0.0013656022	17.453205	28
AGCGGCG	490	0.0	17.30479	31
AACCGCG	315	0.0	17.273752	7
GTCACGC	65	2.1021569E-4	17.229445	24
AGAACCG	400	0.0	17.203897	5
TATACTG	140	4.0017767E-11	17.14674	5
GTCCTAC	1130	0.0	17.13489	1
TCGTTTA	340	0.0	16.939875	30
GATATAC	350	0.0	16.916407	1
CAAGACG	615	0.0	16.651932	4
TGTAGGA	1415	0.0	16.282532	2
ATCGTTT	355	0.0	16.224104	29
AAGACGG	645	0.0	16.127682	5
TCACGCA	60	0.002443609	15.998769	25
>>END_MODULE