FastQCFastQC Report
Thu 2 Feb 2017
SRR4062956_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062956_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1346187
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT45240.336060294743598No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT28480.21156050385273367No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT24030.17850417512574404No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC23840.17709278131492875No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT20980.1558475902679197No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC18410.13675663187952344No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA18340.13623664468606517No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC18130.13467668310569036No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG17610.13081392109714327No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT17310.1285854045537507No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG17020.12643117189513792No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG16980.12613403635601889No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC16700.12405408758218582No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG16680.12390551981262632No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG16510.12264269377137053No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC16110.11967133838018046No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG15700.11662569910421063No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA13960.10370030315253378No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG13930.10347745149819454No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA13560.10072894776134371No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13540.10058037999178421No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAT3900.019.295291
TCTAGCG4550.018.63699528
CAAGACG5450.018.1967434
CCTATTC3900.018.0469023
CTAGCGG4750.017.85294329
AATCCCG1201.6589183E-917.33363519
AAGACGG5700.017.1180155
CGCAAGA5450.017.0284522
GACGGAC5900.016.8113547
CATCGTT3950.016.60726528
ACCGTCG4050.016.5904168
TAGCGGC5050.016.47492230
CGGTCCA5350.016.44949510
GATATAC2550.016.3249281
ACGGACC5900.016.269058
AGACGGA6200.016.2535176
ATCGTTT3950.016.20281429
CGGACCA6050.016.1325139
GTGCTAT1304.7584763E-916.0109861
TACCGTC3900.015.9979027
Produced by FastQC (version 0.11.5)