##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062956_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1346187
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.241756160176855	32.0	32.0	32.0	32.0	32.0
2	30.861496211150456	32.0	32.0	32.0	32.0	32.0
3	30.914388565630183	32.0	32.0	32.0	32.0	32.0
4	30.98098778252947	32.0	32.0	32.0	32.0	32.0
5	30.889547291720987	32.0	32.0	32.0	32.0	32.0
6	34.548826426046304	36.0	36.0	36.0	32.0	36.0
7	34.47145233165972	36.0	36.0	36.0	32.0	36.0
8	34.44315091439748	36.0	36.0	36.0	32.0	36.0
9	34.59184496656111	36.0	36.0	36.0	32.0	36.0
10	34.30917472832526	36.0	36.0	36.0	32.0	36.0
11	34.58059095801698	36.0	36.0	36.0	32.0	36.0
12	34.39594350561995	36.0	36.0	36.0	32.0	36.0
13	34.49943655673395	36.0	36.0	36.0	32.0	36.0
14	34.38225595701043	36.0	36.0	36.0	32.0	36.0
15	34.332182675958094	36.0	36.0	36.0	32.0	36.0
16	34.34259950512076	36.0	36.0	36.0	32.0	36.0
17	34.25922550136051	36.0	36.0	36.0	32.0	36.0
18	34.30307453570715	36.0	36.0	36.0	32.0	36.0
19	34.2807641137524	36.0	36.0	36.0	32.0	36.0
20	34.254390363300196	36.0	36.0	36.0	32.0	36.0
21	34.236535488754534	36.0	36.0	36.0	32.0	36.0
22	34.21775875119876	36.0	36.0	36.0	32.0	36.0
23	34.16986570216471	36.0	36.0	36.0	32.0	36.0
24	34.15541600089735	36.0	36.0	36.0	32.0	36.0
25	34.12459635994108	36.0	36.0	36.0	32.0	36.0
26	34.077503348346106	36.0	36.0	36.0	32.0	36.0
27	34.090517142120675	36.0	36.0	36.0	32.0	36.0
28	34.07692393404482	36.0	36.0	36.0	32.0	36.0
29	34.044077828711764	36.0	36.0	36.0	32.0	36.0
30	34.0168312426134	36.0	36.0	36.0	32.0	36.0
31	34.035278159720754	36.0	36.0	36.0	32.0	36.0
32	33.987792186375295	36.0	36.0	36.0	32.0	36.0
33	33.94694942084569	36.0	36.0	36.0	32.0	36.0
34	33.94295666203878	36.0	36.0	36.0	32.0	36.0
35	33.90921989292721	36.0	36.0	36.0	32.0	36.0
36	33.86135284325283	36.0	36.0	36.0	32.0	36.0
37	33.87354282874519	36.0	36.0	36.0	32.0	36.0
38	33.270510709136246	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	7.0
8	2.0
9	2.0
10	17.0
11	7.0
12	7.0
13	7.0
14	119.0
15	290.0
16	460.0
17	496.0
18	661.0
19	897.0
20	1121.0
21	1789.0
22	2555.0
23	4033.0
24	5967.0
25	8928.0
26	13281.0
27	18662.0
28	25891.0
29	34968.0
30	46892.0
31	63191.0
32	87339.0
33	131145.0
34	293198.0
35	604254.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.19949066719028	17.110027192183882	11.332655016273659	25.357827124352177
2	17.017264548660044	18.925596246811395	37.473168150222676	26.583971054305884
3	19.467854956523805	23.204712545987295	27.8472746399767	29.4801578575122
4	12.481911350015006	14.718069632759548	35.43369674062585	37.366322276599604
5	14.840032893001917	36.308882224779246	33.107980118602	15.743104763616833
6	35.2438929270494	35.179414419266294	16.216270423455914	13.360422230228385
7	31.077108900917928	29.98825571781632	20.033100899057857	18.901534482207897
8	28.956305991769305	32.510734077166504	18.55383381122881	19.979126119835385
9	27.473179703784478	13.883932484612036	18.38228411908642	40.26060369251706
10	16.27688442621672	26.039033442060827	30.56495458627716	27.11912754544529
11	38.61693798201994	21.045969343866027	21.292448583301514	19.044644090812522
12	25.343405521974166	23.415907154408615	27.971049267205935	23.26963805641129
13	29.758632269338097	18.84574694129849	25.221248800145023	26.174371989218393
14	23.844910906977006	19.637981821085905	24.45082518303351	32.06628208890358
15	25.432691521816984	27.191469063317776	22.150893320196925	25.224946094668315
16	26.21456452195258	25.63256015060496	23.35855302747768	24.794322299964787
17	24.25922032107573	25.851893844550133	24.635321896734784	25.253563937639356
18	25.38468453008425	24.50570275825847	26.01219966079862	24.097413050858663
19	25.725732641550923	24.46838542502422	25.357108896298016	24.448773037126845
20	26.07631065379098	23.81600813607874	24.767792657768386	25.339888552361895
21	27.796989671636336	23.6576953106718	24.154761276845164	24.3905537408467
22	26.337642385562237	23.772789304655515	25.05822457935295	24.831343730429296
23	24.695592611908253	23.74177041405348	25.72794040078511	25.834696573253158
24	25.119000300890416	24.66736750606424	25.188019360997917	25.02561283204743
25	25.057412339845392	24.275074017526457	25.302069518895365	25.36544412373279
26	24.71213384271787	25.548873722266553	25.63847666088631	24.10051577412927
27	25.44618977998074	24.462878436686516	24.95980874609231	25.131123037240428
28	24.55430966470893	24.585362012429854	25.740390486986986	25.11993783587423
29	24.301159465726037	24.70537891952088	25.80132550698302	25.192136107770065
30	24.159878764917597	25.28115470473092	25.997199410167553	24.561767120183934
31	25.15177088316836	24.96917037860261	24.497811468322006	25.38124726990702
32	24.84256288384235	24.764039334577923	24.419040883505076	25.974356898074653
33	24.32698672360266	24.36004585189659	25.322400791041794	25.990566633458954
34	25.534312807757342	24.54079707108619	25.217603199933436	24.707286921223034
35	25.860023284132538	24.393360678939747	25.549097646995016	24.197518389932696
36	24.349570392163418	25.418073618401255	24.73367285911166	25.498683130323663
37	25.599512054874513	25.137197420903934	24.77703329660868	24.486257227612878
38	24.615225753539445	24.779847769208256	25.147870091240414	25.457056386011885
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	70.0
1	109.0
2	148.0
3	148.0
4	358.5
5	569.0
6	569.0
7	783.5
8	998.0
9	1061.0
10	1124.0
11	1124.0
12	1546.5
13	1969.0
14	2657.5
15	3346.0
16	3346.0
17	4793.0
18	6240.0
19	6240.0
20	7615.5
21	8991.0
22	9159.5
23	9328.0
24	9328.0
25	10960.5
26	12593.0
27	12593.0
28	16202.5
29	19812.0
30	24921.0
31	30030.0
32	30030.0
33	37728.5
34	45427.0
35	45427.0
36	50681.5
37	55936.0
38	64094.0
39	72252.0
40	72252.0
41	76633.5
42	81015.0
43	91789.0
44	102563.0
45	102563.0
46	103376.0
47	104189.0
48	104189.0
49	111621.5
50	119054.0
51	121944.0
52	124834.0
53	124834.0
54	119824.5
55	114815.0
56	114815.0
57	112080.0
58	109345.0
59	98617.0
60	87889.0
61	87889.0
62	83847.5
63	79806.0
64	67315.0
65	54824.0
66	54824.0
67	46584.5
68	38345.0
69	38345.0
70	30717.5
71	23090.0
72	18142.5
73	13195.0
74	13195.0
75	10185.0
76	7175.0
77	7175.0
78	6750.5
79	6326.0
80	5004.0
81	3682.0
82	3682.0
83	3585.5
84	3489.0
85	3489.0
86	2357.5
87	1226.0
88	1050.5
89	875.0
90	875.0
91	642.0
92	409.0
93	355.5
94	302.0
95	302.0
96	279.0
97	256.0
98	256.0
99	453.0
100	650.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1250197780843226
2	0.05727287516518879
3	0.012108273219099576
4	0.002897071506410328
5	2.97135539119008E-4
6	1.48567769559504E-4
7	0.0
8	5.19987193458264E-4
9	3.7141942389875995E-4
10	5.94271078238016E-4
11	0.002154232658612808
12	0.002822787621630576
13	0.014188221992932631
14	0.009062633943129743
15	0.022953720396943367
16	0.011068298832183047
17	0.020353784429652046
18	0.006239846321499168
19	0.007354104593195448
20	0.006239846321499168
21	0.0065369818606181756
22	0.00787409178665371
23	0.009582621136588008
24	0.013519667029914861
25	0.016491022421104944
26	0.018496687310158248
27	0.009136917827909496
28	0.005422723588921896
29	0.01010260833004627
30	0.003119923160749584
31	0.005868426897600408
32	0.0069826851692966875
33	0.008468362864891728
34	0.011811137679980568
35	0.015302480264628911
36	0.014262505877712384
37	0.009954040560486767
38	0.005422723588921896
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1346187.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.875825410087614
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.35633589555736	35.11995130059448
2	14.854236313377767	14.22317648678915
3	5.347122422120382	7.679936985834006
4	2.401584772428945	4.599114130893327
5	1.2409714094045203	2.9706265267780587
6	0.7439367904826047	2.136995273837167
7	0.4308072602919994	1.4437677225396552
8	0.2902959413037915	1.1118526242493891
9	0.19734893621497418	0.850341889358516
>10	0.9338181110132506	7.976823701440076
>50	0.09184397292848666	3.074013874687594
>100	0.09066715229647024	9.424228895651892
>500	0.014488037776966379	4.560111201075267
>1k	0.006542984802500945	4.82905938627141
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4524	0.336060294743598	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2848	0.21156050385273367	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2403	0.17850417512574404	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2384	0.17709278131492875	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2098	0.1558475902679197	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1841	0.13675663187952344	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1834	0.13623664468606517	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1813	0.13467668310569036	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1761	0.13081392109714327	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1731	0.1285854045537507	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1702	0.12643117189513792	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1698	0.12613403635601889	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1670	0.12405408758218582	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1668	0.12390551981262632	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1651	0.12264269377137053	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1611	0.11967133838018046	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1570	0.11662569910421063	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1396	0.10370030315253378	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1393	0.10347745149819454	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1356	0.10072894776134371	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1354	0.10058037999178421	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.4283884779752E-5
2	0.0	0.0	0.0	0.0	7.4283884779752E-5
3	0.0	0.0	0.0	0.0	7.4283884779752E-5
4	0.0	0.0	0.0	0.0	7.4283884779752E-5
5	0.0	0.0	0.0	0.0	7.4283884779752E-5
6	0.0	0.0	0.0	0.0	7.4283884779752E-5
7	0.0	0.0	0.0	7.4283884779752E-5	7.4283884779752E-5
8	0.0	0.0	0.0	7.4283884779752E-5	7.4283884779752E-5
9	0.0	0.0	0.0	2.97135539119008E-4	7.4283884779752E-5
10	0.0	0.0	0.0	2.97135539119008E-4	1.48567769559504E-4
11	0.0	0.0	0.0	2.97135539119008E-4	1.48567769559504E-4
12	0.0	0.0	0.0	2.97135539119008E-4	4.45703308678512E-4
13	0.0	0.0	0.0	2.97135539119008E-4	5.19987193458264E-4
14	0.0	0.0	0.0	3.7141942389875995E-4	5.19987193458264E-4
15	0.0	0.0	0.0	3.7141942389875995E-4	5.94271078238016E-4
16	0.0	0.0	0.0	4.45703308678512E-4	5.94271078238016E-4
17	0.0	0.0	0.0	4.45703308678512E-4	5.94271078238016E-4
18	0.0	0.0	0.0	4.45703308678512E-4	8.171227325772719E-4
19	0.0	0.0	0.0	4.45703308678512E-4	8.171227325772719E-4
20	0.0	0.0	0.0	5.19987193458264E-4	0.001039974386916528
21	0.0	0.0	0.0	6.68554963017768E-4	0.00111425827169628
22	0.0	0.0	0.0	6.68554963017768E-4	0.001188542156476032
23	0.0	0.0	0.0	8.91406617357024E-4	0.001188542156476032
24	0.0	0.0	0.0	0.00111425827169628	0.0012628260412557838
25	0.0	0.0	0.0	0.001782813234714048	0.0012628260412557838
26	0.0	0.0	0.0	0.0025256520825115676	0.0012628260412557838
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCCTAT	390	0.0	19.29529	1
TCTAGCG	455	0.0	18.636995	28
CAAGACG	545	0.0	18.196743	4
CCTATTC	390	0.0	18.046902	3
CTAGCGG	475	0.0	17.852943	29
AATCCCG	120	1.6589183E-9	17.333635	19
AAGACGG	570	0.0	17.118015	5
CGCAAGA	545	0.0	17.028452	2
GACGGAC	590	0.0	16.811354	7
CATCGTT	395	0.0	16.607265	28
ACCGTCG	405	0.0	16.590416	8
TAGCGGC	505	0.0	16.474922	30
CGGTCCA	535	0.0	16.449495	10
GATATAC	255	0.0	16.324928	1
ACGGACC	590	0.0	16.26905	8
AGACGGA	620	0.0	16.253517	6
ATCGTTT	395	0.0	16.202814	29
CGGACCA	605	0.0	16.132513	9
GTGCTAT	130	4.7584763E-9	16.010986	1
TACCGTC	390	0.0	15.997902	7
>>END_MODULE