FastQCFastQC Report
Thu 2 Feb 2017
SRR4062956_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062956_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1346187
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT51270.3808534772657885No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT35890.2666048624745299No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT23960.1779841879322858No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC23660.17575567138889323No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT22080.1640188175936924No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA20120.14945917617686102No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC19890.14775064682692673No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT19690.1462649691313317No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC19060.14158508439020728No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC18940.14069367777285027No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG17930.13319100541009532No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG17360.12895682397764946No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG16340.12137986773011475No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15710.1166999829889904No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG15520.11528858917817511No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG15470.11491716975427634No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC15410.11447146644559783No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG15280.11350577594346105No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG14710.10927159451101517No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA14700.10919731062623544No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC14040.1042945742307718No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG13610.10110036718524247No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG4350.019.86165228
ATCGTTT3400.018.82313729
AACGAAT1450.018.7582331
CAAGACG5250.018.5900884
CATCGTT3550.018.47848928
TAGCGGC4700.018.38259330
AAGACGG5250.017.9812455
GCATCGT3750.017.91962627
TTCACGA450.00885210217.77740730
CTAGCGG4900.017.63228629
CGCAAGA5100.017.568262
CGGACCA5450.017.3213839
GACGGAC5500.017.1639167
TCGTTTA3650.017.09553330
GGCATCG3850.016.62364826
CTATACT1551.0913936E-1116.5157834
ACGGACC5750.016.4176588
GCGCAAG5800.016.277941
CGAAAAC3450.016.23214723
TAGGTTG805.6212226E-516.0002615
Produced by FastQC (version 0.11.5)