##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062956_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1346187
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.191758648686996	32.0	32.0	32.0	32.0	32.0
2	31.29780335124318	32.0	32.0	32.0	32.0	32.0
3	31.377192767423843	32.0	32.0	32.0	32.0	32.0
4	31.48332661064176	32.0	32.0	32.0	32.0	32.0
5	31.397181817979227	32.0	32.0	32.0	32.0	32.0
6	34.93463315274921	36.0	36.0	36.0	36.0	36.0
7	34.952375115790005	36.0	36.0	36.0	36.0	36.0
8	34.88980579964002	36.0	36.0	36.0	32.0	36.0
9	35.00186155415258	36.0	36.0	36.0	36.0	36.0
10	34.850892186598145	36.0	36.0	36.0	32.0	36.0
11	35.03140796932373	36.0	36.0	36.0	36.0	36.0
12	34.91871857327399	36.0	36.0	36.0	32.0	36.0
13	34.979696728612	36.0	36.0	36.0	36.0	36.0
14	34.92256722134444	36.0	36.0	36.0	32.0	36.0
15	34.88950569274551	36.0	36.0	36.0	32.0	36.0
16	34.8969467094839	36.0	36.0	36.0	32.0	36.0
17	34.85882570549263	36.0	36.0	36.0	32.0	36.0
18	34.86410208982853	36.0	36.0	36.0	32.0	36.0
19	34.86182826011542	36.0	36.0	36.0	32.0	36.0
20	34.8409418602319	36.0	36.0	36.0	32.0	36.0
21	34.82818211734328	36.0	36.0	36.0	32.0	36.0
22	34.82199204122458	36.0	36.0	36.0	32.0	36.0
23	34.76651312187683	36.0	36.0	36.0	32.0	36.0
24	34.73812033543631	36.0	36.0	36.0	32.0	36.0
25	34.72430873273921	36.0	36.0	36.0	32.0	36.0
26	34.657580261880405	36.0	36.0	36.0	32.0	36.0
27	34.64937040693455	36.0	36.0	36.0	32.0	36.0
28	34.61839996969218	36.0	36.0	36.0	32.0	36.0
29	34.578170046212	36.0	36.0	36.0	32.0	36.0
30	34.55657943510077	36.0	36.0	36.0	32.0	36.0
31	34.55287192641141	36.0	36.0	36.0	32.0	36.0
32	34.531838444436026	36.0	36.0	36.0	32.0	36.0
33	34.48560489738796	36.0	36.0	36.0	32.0	36.0
34	34.467234492681925	36.0	36.0	36.0	32.0	36.0
35	34.4478493701098	36.0	36.0	36.0	32.0	36.0
36	34.41288097418858	36.0	36.0	36.0	32.0	36.0
37	34.41688264706166	36.0	36.0	36.0	32.0	36.0
38	33.978115224705036	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	8.0
21	39.0
22	114.0
23	343.0
24	988.0
25	2117.0
26	4616.0
27	8951.0
28	15324.0
29	24332.0
30	36719.0
31	53417.0
32	77024.0
33	125029.0
34	307473.0
35	689693.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.52170130698901	18.367084648580946	12.06630259473683	26.04491144969322
2	16.028308102811867	19.95421141342176	36.67595958065261	27.341520903113757
3	18.51466401027495	23.82157902282521	28.231144707236066	29.432612259663777
4	12.042318074916839	15.485452976037486	35.4899694395871	36.98225950945858
5	14.297599733765963	36.835009181508624	32.79685392190072	16.070537162824696
6	35.279103540746625	35.633066777597435	15.761429521202508	13.32640016045343
7	30.71577458553029	30.211977910796346	20.03806331703534	19.034184186638022
8	28.781136807323964	32.836684604161775	18.393781998520232	19.98839658999403
9	27.213972592359823	13.794748020423128	18.212330095140036	40.77894929207701
10	16.060307121802794	26.386817176951606	30.545783017970905	27.007092683274696
11	38.67859421882185	20.970903934027028	21.198965625085894	19.15153622206523
12	25.047764786284155	23.581913265685124	28.004798763909726	23.365523184120995
13	29.771019584975317	18.94234447717067	25.209090875325458	26.07754506252855
14	23.687011814869425	19.60391773790378	24.472639347489388	32.23643109973741
15	25.306068176263775	27.26263141747766	22.061570940738545	25.36972946552002
16	26.296049508723527	25.647699762365857	23.29475771196721	24.76149301694341
17	24.14798241254744	26.017113521375556	24.637216077706885	25.197687988370117
18	25.353981281946712	24.43553533052986	26.049204159600414	24.161279227923018
19	25.721835079376042	24.43843240203627	25.324564863573933	24.51516765501375
20	25.96370340821892	23.750712196745326	24.864747616787266	25.42083677824849
21	27.576521800495474	23.699565810048394	24.28863789152308	24.435274497933047
22	26.20401177548142	23.787705571365642	25.252212359798452	24.75607029335449
23	24.55847515984035	23.656594514729377	25.809415779531374	25.975514545898896
24	25.077050959487796	24.499345187555665	25.303319672526925	25.120284180429614
25	25.020242373639306	24.216490143264007	25.349023091905575	25.414244391191115
26	24.666000586843563	25.553025772832115	25.627532619959364	24.15344102036496
27	25.46330962186447	24.27558194125023	25.078741093342366	25.182367343542932
28	24.494219599505865	24.42149567630649	25.9443153142914	25.139969409896246
29	24.364445652795638	24.494219599505865	25.89083091724998	25.25050383044852
30	24.19908972527591	25.119244206042694	26.043261448818033	24.638404619863362
31	25.229555774940625	24.70592867112816	24.731110908068494	25.33340464586272
32	24.934500184595453	24.493922463966744	24.74047067755074	25.83110667388706
33	24.280876282418415	24.281247701842315	25.43807063951739	25.99980537622188
34	25.46199005041647	24.409684538626504	25.38614620405635	24.742179206900676
35	25.755039975872595	24.31006984913686	25.736914707986337	24.197975467004213
36	24.347211791526735	25.186025418459696	25.04897165104105	25.417791138972518
37	25.618357627877852	25.02267515582902	24.9196434076395	24.439323808653626
38	24.660521890023947	24.534758992827133	25.340443802630247	25.46427531451867
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	25.0
1	37.5
2	50.0
3	50.0
4	227.5
5	405.0
6	405.0
7	651.5
8	898.0
9	1055.0
10	1212.0
11	1212.0
12	1610.5
13	2009.0
14	2743.5
15	3478.0
16	3478.0
17	5209.5
18	6941.0
19	6941.0
20	8094.0
21	9247.0
22	9384.0
23	9521.0
24	9521.0
25	11131.5
26	12742.0
27	12742.0
28	16269.5
29	19797.0
30	24718.5
31	29640.0
32	29640.0
33	37910.5
34	46181.0
35	46181.0
36	51136.5
37	56092.0
38	64622.0
39	73152.0
40	73152.0
41	77204.5
42	81257.0
43	92386.0
44	103515.0
45	103515.0
46	103306.0
47	103097.0
48	103097.0
49	111144.5
50	119192.0
51	121787.0
52	124382.0
53	124382.0
54	119824.0
55	115266.0
56	115266.0
57	112434.5
58	109603.0
59	98883.5
60	88164.0
61	88164.0
62	84135.0
63	80106.0
64	67322.0
65	54538.0
66	54538.0
67	46466.5
68	38395.0
69	38395.0
70	30692.5
71	22990.0
72	18086.5
73	13183.0
74	13183.0
75	10015.0
76	6847.0
77	6847.0
78	6498.5
79	6150.0
80	4829.5
81	3509.0
82	3509.0
83	3258.0
84	3007.0
85	3007.0
86	1948.5
87	890.0
88	687.0
89	484.0
90	484.0
91	307.5
92	131.0
93	91.5
94	52.0
95	52.0
96	34.0
97	16.0
98	16.0
99	19.5
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008616930634451231
2	0.0
3	0.0
4	7.4283884779752E-5
5	2.22851654339256E-4
6	1.48567769559504E-4
7	6.68554963017768E-4
8	0.002302800428172312
9	0.004902736395463632
10	8.171227325772719E-4
11	0.00445703308678512
12	5.19987193458264E-4
13	1.48567769559504E-4
14	1.48567769559504E-4
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.48567769559504E-4
22	0.0
23	0.0
24	0.0
25	7.4283884779752E-5
26	1.48567769559504E-4
27	2.22851654339256E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	2.22851654339256E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1346187.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.67157966078345
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.12420234045061	32.4835467300157
2	16.09221843977054	14.699140727814171
3	5.816746931291185	7.969800625172491
4	2.5821116068711283	4.7171646378499315
5	1.3381660988097202	3.055807979057397
6	0.7841997665040744	2.1489385263515173
7	0.4752624188740959	1.5194189796369433
8	0.33787236050686276	1.2344931542452902
9	0.21951112403244089	0.9022877808908166
>10	1.011573606963449	8.285798351435844
>50	0.09472110875015699	3.028235796981574
>100	0.10118331014878153	10.050026895674721
>500	0.014548154010312581	4.4426957118127826
>1k	0.007519270612071672	5.079883496859113
>5k	1.634624046102537E-4	0.38276060620182045
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5127	0.3808534772657885	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3589	0.2666048624745299	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2396	0.1779841879322858	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2366	0.17575567138889323	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2208	0.1640188175936924	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2012	0.14945917617686102	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1989	0.14775064682692673	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1969	0.1462649691313317	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1906	0.14158508439020728	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1894	0.14069367777285027	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1793	0.13319100541009532	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1736	0.12895682397764946	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1634	0.12137986773011475	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1571	0.1166999829889904	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1552	0.11528858917817511	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1547	0.11491716975427634	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1541	0.11447146644559783	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	1528	0.11350577594346105	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1471	0.10927159451101517	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1470	0.10919731062623544	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	1404	0.1042945742307718	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1361	0.10110036718524247	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.4283884779752E-5
2	0.0	0.0	0.0	0.0	7.4283884779752E-5
3	0.0	0.0	0.0	0.0	7.4283884779752E-5
4	0.0	0.0	0.0	7.4283884779752E-5	7.4283884779752E-5
5	0.0	0.0	0.0	7.4283884779752E-5	1.48567769559504E-4
6	0.0	0.0	0.0	7.4283884779752E-5	2.22851654339256E-4
7	0.0	0.0	0.0	2.22851654339256E-4	2.22851654339256E-4
8	0.0	0.0	0.0	2.22851654339256E-4	2.22851654339256E-4
9	0.0	0.0	0.0	2.97135539119008E-4	2.22851654339256E-4
10	0.0	0.0	0.0	2.97135539119008E-4	2.22851654339256E-4
11	0.0	0.0	0.0	2.97135539119008E-4	2.22851654339256E-4
12	0.0	0.0	0.0	2.97135539119008E-4	4.45703308678512E-4
13	0.0	0.0	0.0	2.97135539119008E-4	5.19987193458264E-4
14	0.0	0.0	0.0	3.7141942389875995E-4	5.19987193458264E-4
15	0.0	0.0	0.0	3.7141942389875995E-4	7.428388477975199E-4
16	0.0	0.0	0.0	3.7141942389875995E-4	8.171227325772719E-4
17	0.0	0.0	0.0	5.19987193458264E-4	8.171227325772719E-4
18	0.0	0.0	0.0	5.94271078238016E-4	8.91406617357024E-4
19	0.0	0.0	0.0	6.68554963017768E-4	8.91406617357024E-4
20	0.0	0.0	0.0	6.68554963017768E-4	9.656905021367759E-4
21	0.0	0.0	0.0	8.171227325772719E-4	0.001039974386916528
22	0.0	0.0	0.0	8.171227325772719E-4	0.001039974386916528
23	0.0	0.0	0.0	0.001039974386916528	0.001039974386916528
24	0.0	0.0	0.0	0.001188542156476032	0.001039974386916528
25	0.0	0.0	0.0	0.0017085293499342958	0.001039974386916528
26	0.0	0.0	0.0	0.002377084312952064	0.001337109926035536
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	435	0.0	19.861652	28
ATCGTTT	340	0.0	18.823137	29
AACGAAT	145	0.0	18.75823	31
CAAGACG	525	0.0	18.590088	4
CATCGTT	355	0.0	18.478489	28
TAGCGGC	470	0.0	18.382593	30
AAGACGG	525	0.0	17.981245	5
GCATCGT	375	0.0	17.919626	27
TTCACGA	45	0.008852102	17.777407	30
CTAGCGG	490	0.0	17.632286	29
CGCAAGA	510	0.0	17.56826	2
CGGACCA	545	0.0	17.321383	9
GACGGAC	550	0.0	17.163916	7
TCGTTTA	365	0.0	17.095533	30
GGCATCG	385	0.0	16.623648	26
CTATACT	155	1.0913936E-11	16.515783	4
ACGGACC	575	0.0	16.417658	8
GCGCAAG	580	0.0	16.27794	1
CGAAAAC	345	0.0	16.232147	23
TAGGTTG	80	5.6212226E-5	16.000261	5
>>END_MODULE