FastQCFastQC Report
Thu 2 Feb 2017
SRR4062955_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062955_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1440540
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT45170.31356296944201484No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT28490.19777305732572506No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG18430.12793813431074458No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA17600.122176406069946No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG16510.1146097991031141No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC16510.1146097991031141No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG16250.1128049203770808No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT15970.11086120482596805No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA15360.10662668166104376No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT15290.10614075277326558No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG15200.1055159870604079No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC15150.10516889499770918No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC14910.10350285309675539No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG14520.10079553500770544No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG400.0044914319.9942975
TATCTCG450.00885171717.77764132
CTTATAC1201.6625563E-917.3307953
TCTAGCG4750.016.84080728
CTAGCGG4700.016.68072329
CGGTCCA4900.016.65409310
CGTCGTA3000.016.0009910
TAGCGGC4950.015.83826330
CATCGTT3450.015.76690328
GTCCTAT5350.015.5674981
TACCGTC3000.015.4644017
ACCGTCG3050.015.2108868
TCTTATA2000.015.2046362
GTATATA1901.8189894E-1215.1735841
CCTATTC5600.015.1406383
CGCCGGT5200.015.0747157
TATTCCA6500.015.0111025
ATACCGT3200.014.9957226
GTTTTCG4700.014.9775728
CTATTCC5700.014.8739814
Produced by FastQC (version 0.11.5)