##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062955_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1440540
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24598345065045	32.0	32.0	32.0	32.0	32.0
2	30.883248642870036	32.0	32.0	32.0	32.0	32.0
3	30.93749635553334	32.0	32.0	32.0	32.0	32.0
4	30.998706734974384	32.0	32.0	32.0	32.0	32.0
5	30.926131173032335	32.0	32.0	32.0	32.0	32.0
6	34.586650145084484	36.0	36.0	36.0	32.0	36.0
7	34.50800463714996	36.0	36.0	36.0	32.0	36.0
8	34.47680869673872	36.0	36.0	36.0	32.0	36.0
9	34.602107543004706	36.0	36.0	36.0	32.0	36.0
10	34.35566245991087	36.0	36.0	36.0	32.0	36.0
11	34.61897274633124	36.0	36.0	36.0	32.0	36.0
12	34.43999403001652	36.0	36.0	36.0	32.0	36.0
13	34.519194885251366	36.0	36.0	36.0	32.0	36.0
14	34.425536951420995	36.0	36.0	36.0	32.0	36.0
15	34.382645396865065	36.0	36.0	36.0	32.0	36.0
16	34.39168645091424	36.0	36.0	36.0	32.0	36.0
17	34.32439293598234	36.0	36.0	36.0	32.0	36.0
18	34.33902147805684	36.0	36.0	36.0	32.0	36.0
19	34.33004637149958	36.0	36.0	36.0	32.0	36.0
20	34.308377414025294	36.0	36.0	36.0	32.0	36.0
21	34.286950032626656	36.0	36.0	36.0	32.0	36.0
22	34.26866452857956	36.0	36.0	36.0	32.0	36.0
23	34.220036236411346	36.0	36.0	36.0	32.0	36.0
24	34.20189304010996	36.0	36.0	36.0	32.0	36.0
25	34.18589695530843	36.0	36.0	36.0	32.0	36.0
26	34.13711663681675	36.0	36.0	36.0	32.0	36.0
27	34.142135588043374	36.0	36.0	36.0	32.0	36.0
28	34.12316145334388	36.0	36.0	36.0	32.0	36.0
29	34.08445166395935	36.0	36.0	36.0	32.0	36.0
30	34.05892304274786	36.0	36.0	36.0	32.0	36.0
31	34.08871673122579	36.0	36.0	36.0	32.0	36.0
32	34.031167478862095	36.0	36.0	36.0	32.0	36.0
33	33.988609132686356	36.0	36.0	36.0	32.0	36.0
34	33.99219389950991	36.0	36.0	36.0	32.0	36.0
35	33.95188540408458	36.0	36.0	36.0	32.0	36.0
36	33.9105203604204	36.0	36.0	36.0	32.0	36.0
37	33.90950129812431	36.0	36.0	36.0	32.0	36.0
38	33.32296291668402	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	2.0
8	5.0
9	6.0
10	9.0
11	13.0
12	14.0
13	7.0
14	287.0
15	472.0
16	599.0
17	731.0
18	819.0
19	1000.0
20	1368.0
21	1880.0
22	2859.0
23	4260.0
24	6310.0
25	9116.0
26	13478.0
27	18947.0
28	26408.0
29	36182.0
30	48884.0
31	65682.0
32	90032.0
33	134874.0
34	305902.0
35	670393.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.390938951278066	17.28870503427119	11.514729451535914	25.805626562914835
2	16.692018536731734	20.09542027149422	37.510835319808635	25.701725871965415
3	18.60045808087856	24.476640166292107	28.661922009612894	28.260979743216442
4	12.549878237399584	15.622544269105065	36.36522671168782	35.46235078180753
5	14.663232757274208	36.56592863068235	33.44819806530212	15.322640546741315
6	33.443616204501375	35.819534090735544	17.17379201381706	13.563057690946021
7	29.779200701959475	30.805483225376058	21.154720559457257	18.26059551320721
8	28.158466345442974	32.280621131206296	19.829118805331085	19.73179371801965
9	27.919102390289176	14.112029055831263	18.632357239001845	39.33651131487771
10	15.91657237266839	26.34898266610206	31.489382380096213	26.245062581133332
11	37.091627021949975	21.34913984906661	22.456461388834317	19.1027717401491
12	25.125442903000078	23.875942730837156	28.513303750930234	22.48531061523253
13	29.613339378340868	19.48084679169094	25.21108347039902	25.694730359569174
14	23.98956395928056	19.747041258878653	25.268242764301128	30.995152017539656
15	25.26954589640328	26.699486182474658	22.717816969865297	25.31315095125677
16	25.777097392318588	25.749395984337248	23.596392568524536	24.87711405481963
17	24.01475301475857	25.618157428442878	25.20565902539007	25.16143053140848
18	24.95772160405206	24.599223021662645	26.194694443133127	24.248360931152167
19	25.617976904054775	24.93845570685339	25.74148203378723	23.70208535530461
20	25.954528098858	23.86365371932382	25.230726509077023	24.951091672741157
21	27.39395268952169	23.947024593839945	24.411743062198966	24.247279654439403
22	26.079911470204152	24.08535294244244	24.95572468844135	24.879010898912053
23	24.418886391864376	23.919574057141826	25.508250941938275	26.15328860905552
24	24.681823957053957	25.114714170957964	25.263639513191176	24.939822358796906
25	24.900800469908955	24.276407557146914	25.4057720761047	25.41701989683943
26	24.70222369726556	25.192760468776797	25.73641268688479	24.368603147072854
27	25.529715439365287	24.67335371661682	24.80845545252833	24.988475391489562
28	24.456039554196106	24.565310659000563	25.799643723437192	25.17900606336613
29	24.532624633431084	24.890723584821824	25.69884142006576	24.87781036168133
30	24.506750100833255	25.092450730546474	25.894257329241228	24.506541839379047
31	25.073292915492555	24.935558466676802	24.580670108639435	25.410478509191204
32	24.691067476416993	24.87399718420154	24.654967496133136	25.77996784324833
33	24.391246332621034	24.44407880577783	25.294049855526445	25.870625006074693
34	25.18322113729019	24.65987103009298	25.474958620766262	24.68194921185057
35	25.98832869883391	24.49834581350219	25.242851241247394	24.270474246416505
36	24.700100949929112	25.18742388833577	24.91012401428018	25.202351147454937
37	25.65669988003199	25.04110015107083	24.845874433484404	24.45632553541278
38	24.655767294942553	24.849248498733033	25.467458086014787	25.027526120309624
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	690.0
1	544.5
2	399.0
3	399.0
4	807.5
5	1216.0
6	1216.0
7	1468.5
8	1721.0
9	1614.5
10	1508.0
11	1508.0
12	1967.0
13	2426.0
14	2970.5
15	3515.0
16	3515.0
17	5240.0
18	6965.0
19	6965.0
20	8525.5
21	10086.0
22	10675.0
23	11264.0
24	11264.0
25	12937.5
26	14611.0
27	14611.0
28	18359.5
29	22108.0
30	26788.0
31	31468.0
32	31468.0
33	39972.5
34	48477.0
35	48477.0
36	54095.5
37	59714.0
38	68596.0
39	77478.0
40	77478.0
41	83338.5
42	89199.0
43	101401.0
44	113603.0
45	113603.0
46	117242.5
47	120882.0
48	120882.0
49	127523.5
50	134165.0
51	134547.0
52	134929.0
53	134929.0
54	128788.0
55	122647.0
56	122647.0
57	118862.5
58	115078.0
59	102870.0
60	90662.0
61	90662.0
62	85875.0
63	81088.0
64	66829.0
65	52570.0
66	52570.0
67	43853.5
68	35137.0
69	35137.0
70	28558.0
71	21979.0
72	17067.0
73	12155.0
74	12155.0
75	9243.0
76	6331.0
77	6331.0
78	6156.0
79	5981.0
80	4725.0
81	3469.0
82	3469.0
83	3506.5
84	3544.0
85	3544.0
86	2350.0
87	1156.0
88	996.5
89	837.0
90	837.0
91	605.5
92	374.0
93	317.5
94	261.0
95	261.0
96	227.5
97	194.0
98	194.0
99	423.5
100	653.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1195385063934358
2	0.05581240368195261
3	0.012703569494772792
4	0.002221389201271745
5	3.4709206269871017E-4
6	1.3883682507948407E-4
7	6.941841253974204E-5
8	4.8592888777819433E-4
9	2.082552376192261E-4
10	6.941841253974203E-4
11	0.0025684812639704554
12	0.0030544101517486497
13	0.016174490121759897
14	0.010204506643342081
15	0.023602260263512295
16	0.012495314257153568
17	0.020270176461604675
18	0.006108820303497299
19	0.007913699029530593
20	0.006247657128576784
21	0.00680300442889472
22	0.008399627917308788
23	0.011037527593818985
24	0.015341469171282991
25	0.018118205672872673
26	0.019506573923667513
27	0.009996251405722854
28	0.005969983478417816
29	0.010829272356199758
30	0.002984991739208908
31	0.0060394018909575575
32	0.006664167603815235
33	0.009579740930484401
34	0.014161356158107376
35	0.01770169519763422
36	0.015410887583822733
37	0.010829272356199758
38	0.006247657128576784
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1440540.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.56398333337456
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.48783882788064	44.72224935171697
2	12.128924511534848	13.478627188213608
3	3.4247726416186475	5.70882029938487
4	1.3084390513915987	2.9080834257703683
5	0.7037944448708928	1.9552811402463943
6	0.41463377771147614	1.3823222588528754
7	0.2665017938526811	1.0365530862361356
8	0.20686028378428825	0.9195185088421858
9	0.14698204341459134	0.7350217029534319
>10	0.7424949464193815	7.5739077104752885
>50	0.07497717116353604	2.9037781352084617
>100	0.07946867101668699	9.51611347066963
>500	0.01016893563690225	3.767680878047001
>1k	0.00414289970392314	3.3920428433827947
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4517	0.31356296944201484	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2849	0.19777305732572506	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1843	0.12793813431074458	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1760	0.122176406069946	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1651	0.1146097991031141	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1651	0.1146097991031141	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1625	0.1128049203770808	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1597	0.11086120482596805	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1536	0.10662668166104376	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1529	0.10614075277326558	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1520	0.1055159870604079	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1515	0.10516889499770918	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1491	0.10350285309675539	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1452	0.10079553500770544	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	6.941841253974204E-5	0.0
3	0.0	0.0	0.0	6.941841253974204E-5	0.0
4	0.0	0.0	0.0	6.941841253974204E-5	0.0
5	0.0	0.0	0.0	6.941841253974204E-5	0.0
6	0.0	0.0	0.0	6.941841253974204E-5	0.0
7	0.0	0.0	0.0	6.941841253974204E-5	0.0
8	0.0	0.0	0.0	6.941841253974204E-5	0.0
9	0.0	0.0	0.0	6.941841253974204E-5	0.0
10	0.0	0.0	0.0	6.941841253974204E-5	0.0
11	0.0	0.0	0.0	1.3883682507948407E-4	0.0
12	0.0	0.0	0.0	1.3883682507948407E-4	0.0
13	0.0	0.0	0.0	1.3883682507948407E-4	0.0
14	0.0	0.0	0.0	1.3883682507948407E-4	0.0
15	0.0	0.0	0.0	1.3883682507948407E-4	0.0
16	0.0	0.0	0.0	1.3883682507948407E-4	0.0
17	0.0	0.0	0.0	2.0825523761922613E-4	0.0
18	0.0	0.0	0.0	2.7767365015896815E-4	0.0
19	0.0	0.0	0.0	4.859288877781943E-4	0.0
20	0.0	0.0	0.0	6.247657128576784E-4	0.0
21	0.0	0.0	0.0	9.024393630166466E-4	0.0
22	0.0	0.0	0.0	0.0012495314257153568	0.0
23	0.0	0.0	0.0	0.002082552376192261	0.0
24	0.0	0.0	0.0	0.0029155733266691655	0.0
25	0.0	0.0	0.0	0.0036097574520665863	0.0
26	0.0	0.0	0.0	0.004165104752384522	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGATCG	40	0.00449143	19.994297	5
TATCTCG	45	0.008851717	17.777641	32
CTTATAC	120	1.6625563E-9	17.330795	3
TCTAGCG	475	0.0	16.840807	28
CTAGCGG	470	0.0	16.680723	29
CGGTCCA	490	0.0	16.654093	10
CGTCGTA	300	0.0	16.00099	10
TAGCGGC	495	0.0	15.838263	30
CATCGTT	345	0.0	15.766903	28
GTCCTAT	535	0.0	15.567498	1
TACCGTC	300	0.0	15.464401	7
ACCGTCG	305	0.0	15.210886	8
TCTTATA	200	0.0	15.204636	2
GTATATA	190	1.8189894E-12	15.173584	1
CCTATTC	560	0.0	15.140638	3
CGCCGGT	520	0.0	15.074715	7
TATTCCA	650	0.0	15.011102	5
ATACCGT	320	0.0	14.995722	6
GTTTTCG	470	0.0	14.97757	28
CTATTCC	570	0.0	14.873981	4
>>END_MODULE