FastQCFastQC Report
Thu 2 Feb 2017
SRR4062955_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062955_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1440540
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT51760.3593097033057048No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT35010.24303386230163687No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT21000.1457786663334583No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG19580.13592125175281491No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA18210.12641092923487027No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC16940.11759479084232302No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC16670.11572049370374998No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA16470.11433212545295514No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA16450.11419328862787566No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG16190.11238840990184236No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG15720.10912574451247448No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC15430.10711261054882197No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14910.10350285309675539No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT14820.10287808738389771No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG14730.10225332167104002No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG14670.10183681119580157No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAAT753.2355456E-517.06609217
TCTAGCG3650.015.34194928
GTATTAT1052.1833712E-615.2396981
CGGTCCA4050.015.01288310
CGTCGTA3450.014.84111110
GTCGTGC650.0041631914.76873530
CTAGCGG3850.014.54496629
GTAATAC1553.1322998E-914.4521293
GTCCTAC1351.3175668E-714.223721
CCTATAC901.5341437E-414.2227323
TAGCGGC4050.014.22174430
TGTAATA1707.585186E-1014.1181532
TACGCTA4700.013.9579479
CCCTTAC1503.1664968E-813.8681251
AGGGGCG3600.013.77731532
TATTACC1053.6828013E-513.7147762
CATCGTT4200.013.71382528
TTCGTTC4100.013.65807629
ATACCGT3750.013.6547716
ACCGTCG3650.013.598
Produced by FastQC (version 0.11.5)