##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062955_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1440540
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.169633609618614	32.0	32.0	32.0	32.0	32.0
2	31.299165590681273	32.0	32.0	32.0	32.0	32.0
3	31.37460535632471	32.0	32.0	32.0	32.0	32.0
4	31.477992280672524	32.0	32.0	32.0	32.0	32.0
5	31.40391242173074	32.0	32.0	32.0	32.0	32.0
6	34.928776708734226	36.0	36.0	36.0	36.0	36.0
7	34.955479889485886	36.0	36.0	36.0	36.0	36.0
8	34.892519471864716	36.0	36.0	36.0	36.0	36.0
9	34.99994863037472	36.0	36.0	36.0	36.0	36.0
10	34.85126619184472	36.0	36.0	36.0	32.0	36.0
11	35.0258049064934	36.0	36.0	36.0	36.0	36.0
12	34.92974162466853	36.0	36.0	36.0	32.0	36.0
13	34.970883835228456	36.0	36.0	36.0	36.0	36.0
14	34.91660071917475	36.0	36.0	36.0	32.0	36.0
15	34.88676190872867	36.0	36.0	36.0	32.0	36.0
16	34.89801532758549	36.0	36.0	36.0	32.0	36.0
17	34.86682841156789	36.0	36.0	36.0	32.0	36.0
18	34.87038610521055	36.0	36.0	36.0	32.0	36.0
19	34.859400641426134	36.0	36.0	36.0	32.0	36.0
20	34.84759326363725	36.0	36.0	36.0	32.0	36.0
21	34.83674733086204	36.0	36.0	36.0	32.0	36.0
22	34.81895747428048	36.0	36.0	36.0	32.0	36.0
23	34.76261818484735	36.0	36.0	36.0	32.0	36.0
24	34.733762339122826	36.0	36.0	36.0	32.0	36.0
25	34.723134380162996	36.0	36.0	36.0	32.0	36.0
26	34.65981368098074	36.0	36.0	36.0	32.0	36.0
27	34.64014883307649	36.0	36.0	36.0	32.0	36.0
28	34.609158371166366	36.0	36.0	36.0	32.0	36.0
29	34.574965637885796	36.0	36.0	36.0	32.0	36.0
30	34.55261499160037	36.0	36.0	36.0	32.0	36.0
31	34.54689144348647	36.0	36.0	36.0	32.0	36.0
32	34.519557943548946	36.0	36.0	36.0	32.0	36.0
33	34.47802213058992	36.0	36.0	36.0	32.0	36.0
34	34.464455690227275	36.0	36.0	36.0	32.0	36.0
35	34.43062115595541	36.0	36.0	36.0	32.0	36.0
36	34.404101239812846	36.0	36.0	36.0	32.0	36.0
37	34.393155344523585	36.0	36.0	36.0	32.0	36.0
38	33.967291432379525	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	5.0
21	31.0
22	122.0
23	363.0
24	958.0
25	2417.0
26	4974.0
27	9692.0
28	16810.0
29	26607.0
30	40186.0
31	57516.0
32	82529.0
33	131782.0
34	323252.0
35	743294.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.81920746671628	18.489897731146286	12.195201535108882	26.495693267028553
2	15.78581642994988	21.130131756147	36.67333083427049	26.41072097963264
3	17.64859011204132	25.162230830105376	29.002526830216446	28.18665222763686
4	12.109364695690083	16.514663271638792	36.27714090152429	35.09883113114684
5	14.029897163420888	37.16486479357018	33.1778127338536	15.62742530915533
6	33.412794552024074	36.24361782254509	16.861582675880836	13.482004949549994
7	29.59902369537963	30.95497919521511	21.169024819962598	18.276972289442664
8	28.027929074922287	32.476365943633695	19.721472871338268	19.774232110105753
9	27.837206477030147	13.923671011298355	18.420590430240026	39.81853208143147
10	15.67602089515975	26.612519741066627	31.538779264504264	26.172680099269364
11	37.21107500404382	21.206565361698168	22.48718993605555	19.09516969820246
12	24.950001284243307	23.96758986405764	28.517490352556024	22.564918499143026
13	29.516125225436607	19.45314875414602	25.20398628845612	25.82673973196125
14	23.855915142932513	19.71725880572563	25.200966304302554	31.2258597470393
15	25.257334055284826	26.793702361614397	22.652477543143544	25.296486039957237
16	25.723617532314268	25.74562316908937	23.593582961944826	24.937176336651532
17	24.048759492967914	25.661071542615964	25.192011329084927	25.098157635331198
18	25.013606008857792	24.471656462160023	26.23585599844503	24.27888153053716
19	25.65878073500215	24.934260763324865	25.655240395962625	23.75171810571036
20	25.984353089813546	23.832798811556778	25.27239785080595	24.910450247823732
21	27.298027128715617	24.0309189609452	24.415149874352675	24.255904035986507
22	25.880729365883187	24.122706847922895	25.08630450130125	24.910259284892668
23	24.293230352931865	23.801398510134792	25.65786413778789	26.247506999145454
24	24.686992377858303	24.953697918835992	25.34417648937204	25.015133213933666
25	24.906146306246267	24.197384314215505	25.48898329792994	25.407486081608287
26	24.772533058806463	25.018274409786894	25.80978369901821	24.399408832388435
27	25.531572232041082	24.54527405733136	24.85793502149891	25.065218689128642
28	24.54503172421453	24.39481027947853	25.998861538034348	25.06129645827259
29	24.50768461826815	24.699210018465298	25.8658558596082	24.92724950365835
30	24.621947325308565	24.794104988407124	25.9470754022797	24.63687228400461
31	25.073236425229428	24.581754064448056	24.902328293556582	25.442681216765934
32	24.762172518638845	24.673108695350354	24.883862995821012	25.68085579018979
33	24.51899981951213	24.25229427853444	25.48106959890041	25.747636303053024
34	25.16132839074236	24.41674649784109	25.696960861899008	24.72496424951754
35	25.975189859358295	24.286309300678912	25.385133352770488	24.353367487192305
36	24.7848029211268	25.042275813236703	25.07288933316673	25.10003193246977
37	25.697863301262025	24.898371444041818	24.88455717994641	24.519208074749745
38	24.70434698099324	24.54565648992739	25.639690671553723	25.11030585752565
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	289.0
1	247.5
2	206.0
3	206.0
4	688.0
5	1170.0
6	1170.0
7	1381.5
8	1593.0
9	1537.0
10	1481.0
11	1481.0
12	1943.0
13	2405.0
14	3062.0
15	3719.0
16	3719.0
17	5643.5
18	7568.0
19	7568.0
20	9074.0
21	10580.0
22	10945.0
23	11310.0
24	11310.0
25	12908.5
26	14507.0
27	14507.0
28	18218.0
29	21929.0
30	26755.5
31	31582.0
32	31582.0
33	40314.5
34	49047.0
35	49047.0
36	54454.0
37	59861.0
38	68592.5
39	77324.0
40	77324.0
41	83219.5
42	89115.0
43	101448.0
44	113781.0
45	113781.0
46	117795.0
47	121809.0
48	121809.0
49	128547.0
50	135285.0
51	135020.5
52	134756.0
53	134756.0
54	128498.0
55	122240.0
56	122240.0
57	119168.0
58	116096.0
59	103195.0
60	90294.0
61	90294.0
62	85804.5
63	81315.0
64	66786.5
65	52258.0
66	52258.0
67	43675.5
68	35093.0
69	35093.0
70	28453.5
71	21814.0
72	17057.5
73	12301.0
74	12301.0
75	9254.5
76	6208.0
77	6208.0
78	6015.5
79	5823.0
80	4541.5
81	3260.0
82	3260.0
83	3151.0
84	3042.0
85	3042.0
86	1907.0
87	772.0
88	654.0
89	536.0
90	536.0
91	314.5
92	93.0
93	63.5
94	34.0
95	34.0
96	29.5
97	25.0
98	25.0
99	22.0
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008399627917308788
2	0.0
3	0.0
4	1.3883682507948407E-4
5	1.3883682507948407E-4
6	3.4709206269871017E-4
7	4.165104752384522E-4
8	0.0023602260263512293
9	0.004512196815083233
10	0.0010412761880961305
11	0.004928707290321684
12	2.082552376192261E-4
13	1.3883682507948407E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	6.941841253974204E-5
23	4.8592888777819433E-4
24	0.0
25	0.0
26	6.941841253974204E-5
27	1.3883682507948407E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1440540.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.77416072351816
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.06280844476252	42.51536168561389
2	13.027049867971263	14.010373467071455
3	3.6630255014240056	5.909283661437605
4	1.4473393375483963	3.113178326351911
5	0.7389794584660386	1.9869000085465587
6	0.44289814255587223	1.4289885541168283
7	0.27908436015625376	1.0505269066923832
8	0.208557647735818	0.8972009975571809
9	0.14942433734473884	0.7231651499143066
>10	0.804296758404695	7.84936164207477
>50	0.07469785472450173	2.815057575652581
>100	0.08573126605001334	9.71254093839002
>500	0.011820476450702957	4.356622578096523
>1k	0.004156651059587854	3.2698942256566763
>5k	1.2989534561212043E-4	0.3615442828273974
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5176	0.3593097033057048	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3501	0.24303386230163687	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2100	0.1457786663334583	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1958	0.13592125175281491	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1821	0.12641092923487027	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1694	0.11759479084232302	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1667	0.11572049370374998	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1647	0.11433212545295514	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1645	0.11419328862787566	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1619	0.11238840990184236	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1572	0.10912574451247448	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1543	0.10711261054882197	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1491	0.10350285309675539	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1482	0.10287808738389771	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1473	0.10225332167104002	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1467	0.10183681119580157	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	1.3883682507948407E-4	0.0	0.0	6.941841253974204E-5	0.0
12	1.3883682507948407E-4	0.0	0.0	6.941841253974204E-5	2.0825523761922613E-4
13	1.3883682507948407E-4	0.0	0.0	6.941841253974204E-5	2.0825523761922613E-4
14	1.3883682507948407E-4	0.0	0.0	6.941841253974204E-5	2.0825523761922613E-4
15	1.3883682507948407E-4	0.0	0.0	6.941841253974204E-5	2.0825523761922613E-4
16	1.3883682507948407E-4	0.0	0.0	6.941841253974204E-5	2.0825523761922613E-4
17	1.3883682507948407E-4	0.0	0.0	1.3883682507948407E-4	2.0825523761922613E-4
18	1.3883682507948407E-4	0.0	0.0	2.0825523761922613E-4	2.0825523761922613E-4
19	1.3883682507948407E-4	0.0	0.0	4.1651047523845225E-4	2.0825523761922613E-4
20	1.3883682507948407E-4	0.0	0.0	4.1651047523845225E-4	2.0825523761922613E-4
21	1.3883682507948407E-4	0.0	0.0	6.941841253974205E-4	2.0825523761922613E-4
22	1.3883682507948407E-4	0.0	0.0	0.0010412761880961305	2.7767365015896815E-4
23	1.3883682507948407E-4	0.0	0.0	0.0018048787260332932	2.7767365015896815E-4
24	1.3883682507948407E-4	0.0	0.0	0.0027073180890499395	2.7767365015896815E-4
25	1.3883682507948407E-4	0.0	0.0	0.003262665389367876	2.7767365015896815E-4
26	2.7767365015896815E-4	0.0	0.0	0.00374859427714607	2.7767365015896815E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACAAT	75	3.2355456E-5	17.066092	17
TCTAGCG	365	0.0	15.341949	28
GTATTAT	105	2.1833712E-6	15.239698	1
CGGTCCA	405	0.0	15.012883	10
CGTCGTA	345	0.0	14.841111	10
GTCGTGC	65	0.00416319	14.768735	30
CTAGCGG	385	0.0	14.544966	29
GTAATAC	155	3.1322998E-9	14.452129	3
GTCCTAC	135	1.3175668E-7	14.22372	1
CCTATAC	90	1.5341437E-4	14.222732	3
TAGCGGC	405	0.0	14.221744	30
TGTAATA	170	7.585186E-10	14.118153	2
TACGCTA	470	0.0	13.957947	9
CCCTTAC	150	3.1664968E-8	13.868125	1
AGGGGCG	360	0.0	13.777315	32
TATTACC	105	3.6828013E-5	13.714776	2
CATCGTT	420	0.0	13.713825	28
TTCGTTC	410	0.0	13.658076	29
ATACCGT	375	0.0	13.654771	6
ACCGTCG	365	0.0	13.59	8
>>END_MODULE