FastQCFastQC Report
Thu 2 Feb 2017
SRR4062954_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062954_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1525771
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT31900.2090746252222647No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA23730.1555279265368132No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT23370.1531684636816403No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG23260.15244751669811524No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC22900.15008805384294235No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG22600.14812183479696495No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG22510.14753196908317173No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC22070.14464818114907152No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC20770.13612789861650273No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG20350.13337519195213435No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT19900.13042586338316825No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG19790.12970491639964318No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA19690.12904951005098406No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG17650.11567922053833765No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT17500.11469611101534896No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA17440.11430286720615349No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT17270.11318867641343296No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA16650.1091251570517463No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC16510.1082075881636235No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT15940.10447177197626643No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG15900.10420960943680277No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC15880.10407852816707093No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT15710.1029643373743504No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT15660.10263663420002084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTATA1250.020.4920672
CCTATAC852.4006113E-718.8210413
GTATTAA1453.6379788E-1217.6760121
ACTAGAC550.001366132317.452243
AGAACCG4000.017.1971655
ATCGTTT3800.016.84098429
AACCGCG3450.016.694547
CATCGTT3900.016.40916328
CGGTCCT3650.016.21916212
TCGTTTA3750.016.21225530
GTATAGA1304.7293724E-916.0188871
GATTAGA805.5663466E-516.0188851
AAGACGG6050.015.8651515
CGCGGTC3650.015.78080610
TACCGTC4000.015.5989627
GTATCAA30650.015.156531
ATACCGT4050.015.0098716
ACCGTCG3750.014.932348
CTAAGAC1401.281478E-814.855183
GGTCCTA4000.014.79998513
Produced by FastQC (version 0.11.5)