##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062954_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1525771
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.259406555767544	32.0	32.0	32.0	32.0	32.0
2	30.889872726641155	32.0	32.0	32.0	32.0	32.0
3	30.92917941158929	32.0	32.0	32.0	32.0	32.0
4	31.001870529719074	32.0	32.0	32.0	32.0	32.0
5	30.920090891752434	32.0	32.0	32.0	32.0	32.0
6	34.570592834704556	36.0	36.0	36.0	32.0	36.0
7	34.500585605572525	36.0	36.0	36.0	32.0	36.0
8	34.49566743633219	36.0	36.0	36.0	32.0	36.0
9	34.62233782133754	36.0	36.0	36.0	32.0	36.0
10	34.34282143257409	36.0	36.0	36.0	32.0	36.0
11	34.59449812586555	36.0	36.0	36.0	32.0	36.0
12	34.4213437009879	36.0	36.0	36.0	32.0	36.0
13	34.5001825306681	36.0	36.0	36.0	32.0	36.0
14	34.408135952249715	36.0	36.0	36.0	32.0	36.0
15	34.349545246304984	36.0	36.0	36.0	32.0	36.0
16	34.359209212915964	36.0	36.0	36.0	32.0	36.0
17	34.2917351293215	36.0	36.0	36.0	32.0	36.0
18	34.311917712422115	36.0	36.0	36.0	32.0	36.0
19	34.301985684614536	36.0	36.0	36.0	32.0	36.0
20	34.288925402304805	36.0	36.0	36.0	32.0	36.0
21	34.26661274857105	36.0	36.0	36.0	32.0	36.0
22	34.2430554781812	36.0	36.0	36.0	32.0	36.0
23	34.1979628659871	36.0	36.0	36.0	32.0	36.0
24	34.170287022102265	36.0	36.0	36.0	32.0	36.0
25	34.154653614467705	36.0	36.0	36.0	32.0	36.0
26	34.09926456853617	36.0	36.0	36.0	32.0	36.0
27	34.09821133053387	36.0	36.0	36.0	32.0	36.0
28	34.086881976390956	36.0	36.0	36.0	32.0	36.0
29	34.05937784896947	36.0	36.0	36.0	32.0	36.0
30	34.031726255119544	36.0	36.0	36.0	32.0	36.0
31	34.04869603629903	36.0	36.0	36.0	32.0	36.0
32	34.00196556363963	36.0	36.0	36.0	32.0	36.0
33	33.96320155514818	36.0	36.0	36.0	32.0	36.0
34	33.97941303118227	36.0	36.0	36.0	32.0	36.0
35	33.93424832428982	36.0	36.0	36.0	32.0	36.0
36	33.8953715859064	36.0	36.0	36.0	32.0	36.0
37	33.89990634243278	36.0	36.0	36.0	32.0	36.0
38	33.314160512947225	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	1.0
8	6.0
9	4.0
10	16.0
11	15.0
12	6.0
13	8.0
14	244.0
15	476.0
16	615.0
17	677.0
18	843.0
19	1048.0
20	1340.0
21	1899.0
22	2908.0
23	4507.0
24	6627.0
25	9999.0
26	14558.0
27	20496.0
28	28943.0
29	39004.0
30	53278.0
31	70416.0
32	95853.0
33	145017.0
34	324786.0
35	702179.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.452069770387155	17.18523301527056	11.362787770692947	25.999909443649344
2	17.14935675567208	19.559899955801132	36.648007837757376	26.64273545076942
3	18.764445155276814	23.482988310157722	28.522848089732683	29.22971844483278
4	12.733954502911404	15.004745288138615	35.74086794203932	36.52043226691066
5	15.067352921707023	35.996798991197224	33.14210735701145	15.793740730084306
6	34.665757832955926	35.132408988278016	16.567623454463824	13.634209724302234
7	30.70561093742831	30.179974701298363	20.77606716608663	18.338347195186692
8	28.578815976006762	31.90416860285641	19.533374449373856	19.98364097176298
9	27.651928505466433	14.034384018005369	18.5677760759015	39.745911400626696
10	16.41156958602243	25.732111034573613	30.6815820847198	27.174737294684153
11	38.005264344552394	20.821098800841035	22.042607633299603	19.131029221306964
12	25.311081209154718	23.388063526436905	27.86573220504061	23.43512305936776
13	29.479069706464596	18.637728279822227	25.26475870839178	26.618443305321392
14	24.124575339646352	19.57865302946781	24.408982250622856	31.887789380262987
15	25.57589608237258	26.5326525768965	22.015757570989706	25.875693769741215
16	26.512523630109154	25.25635882632099	23.067312709262477	25.163804834307378
17	24.61454549507115	25.332190031906237	24.68803407877373	25.36523039424888
18	25.852979751337273	23.814021410766735	25.675550786828964	24.657448051067032
19	26.11852398553009	24.39971448216464	24.99664077386083	24.485120758444438
20	26.21433740056945	23.58037816381855	24.425308451007023	25.77997598460498
21	27.770556939640567	23.655334648159474	23.796583418377544	24.777524993822414
22	26.490826289405796	23.601461396480484	24.446142794219437	25.46156951989429
23	24.611321699585545	23.303524667692262	25.201967201509685	26.883186431212515
24	25.300549313685643	24.47618548186216	24.624591947769314	25.59867325668288
25	25.397281143026458	23.820349342573618	24.70517747038009	26.07719204401984
26	25.220982523304446	24.891114811270043	25.184468947071704	24.70343371835381
27	26.08784851561532	24.02112150304559	24.16912409079677	25.721905890542317
28	24.88657531517053	24.027156379900923	25.36210035905254	25.724167945876008
29	24.86913191162455	24.37994155673048	25.253182064287593	25.497744467357375
30	24.859115511516546	24.528123807938297	25.57536844947756	25.0373922310676
31	25.635637518655702	24.29314800746949	23.989019927599166	26.082194546275645
32	25.16120828543076	24.26854894845923	23.952424711568586	26.617818054541427
33	24.818323456675216	23.729542339630754	24.878101217119774	26.57403298657426
34	25.82993902702816	24.020807632961326	24.940908262617707	25.208345077392806
35	26.66556541221986	23.74265256798771	24.98851221179745	24.603269807994977
36	25.107126633283755	24.66006918246827	24.396161074406223	25.836643109841756
37	26.243222480912827	24.38006019800319	24.32506659535196	25.05165072573202
38	25.100381600835824	24.084964192968634	24.963983225818293	25.85067098037725
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	433.0
1	346.0
2	259.0
3	259.0
4	585.0
5	911.0
6	911.0
7	1018.0
8	1125.0
9	1043.5
10	962.0
11	962.0
12	1288.0
13	1614.0
14	2168.0
15	2722.0
16	2722.0
17	4051.5
18	5381.0
19	5381.0
20	6619.0
21	7857.0
22	8545.5
23	9234.0
24	9234.0
25	10796.5
26	12359.0
27	12359.0
28	15803.0
29	19247.0
30	23591.0
31	27935.0
32	27935.0
33	37678.0
34	47421.0
35	47421.0
36	52972.5
37	58524.0
38	67726.5
39	76929.0
40	76929.0
41	82444.0
42	87959.0
43	101666.0
44	115373.0
45	115373.0
46	118902.0
47	122431.0
48	122431.0
49	132235.0
50	142039.0
51	145970.5
52	149902.0
53	149902.0
54	143078.5
55	136255.0
56	136255.0
57	132909.0
58	129563.0
59	116733.0
60	103903.0
61	103903.0
62	99802.5
63	95702.0
64	78814.5
65	61927.0
66	61927.0
67	51739.5
68	41552.0
69	41552.0
70	33362.0
71	25172.0
72	19494.5
73	13817.0
74	13817.0
75	10442.5
76	7068.0
77	7068.0
78	7149.5
79	7231.0
80	5736.5
81	4242.0
82	4242.0
83	4367.0
84	4492.0
85	4492.0
86	2955.0
87	1418.0
88	1230.0
89	1042.0
90	1042.0
91	748.0
92	454.0
93	402.5
94	351.0
95	351.0
96	305.5
97	260.0
98	260.0
99	482.5
100	705.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12177449958086764
2	0.05538183646169707
3	0.011338529831803069
4	0.0030804098386979437
5	1.3108126973182738E-4
6	3.9324380919548215E-4
7	6.554063486591369E-5
8	5.243250789273095E-4
9	2.6216253946365477E-4
10	7.864876183909643E-4
11	0.002293922220306979
12	0.002883787934100202
13	0.015139886654026062
14	0.009175688881227917
15	0.026806119660158702
16	0.011469611101534896
17	0.023856791091192587
18	0.005898657137932232
19	0.00799595745364147
20	0.005439872693870836
21	0.006160819677395887
22	0.007012847930652764
23	0.00996217649961888
24	0.015139886654026062
25	0.01815475585785809
26	0.020383137443299157
27	0.00799595745364147
28	0.005833116503066319
29	0.012387179989657687
30	0.0034736536478934257
31	0.006422982216859542
32	0.006881766660920938
33	0.007864876183909643
34	0.011600692371266723
35	0.01559867109808746
36	0.015729752367819285
37	0.009110148246362004
38	0.006095279042529973
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1525771.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.858210468343756
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.23293362373172	41.31361635196746
2	11.016106446460833	11.205189203915669
3	3.1225711004635346	4.764251346892267
4	1.39073931255057	2.829220506571864
5	0.7819727787589886	1.9884868081320128
6	0.49848325470227717	1.5211179769556051
7	0.34005305675444525	1.2106142951574856
8	0.24966128209240052	1.0157860824361475
9	0.18422376814719957	0.8432362056331473
>10	0.9881770781072077	9.042224087383563
>50	0.08039041777009952	2.935418430485671
>100	0.0922940250305612	10.43645979549048
>500	0.01579219862709167	5.617203253519074
>1k	0.006601656803128485	5.277175655459584
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3190	0.2090746252222647	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2373	0.1555279265368132	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2337	0.1531684636816403	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2326	0.15244751669811524	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2290	0.15008805384294235	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2260	0.14812183479696495	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2251	0.14753196908317173	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2207	0.14464818114907152	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2077	0.13612789861650273	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2035	0.13337519195213435	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1990	0.13042586338316825	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1979	0.12970491639964318	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1969	0.12904951005098406	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1765	0.11567922053833765	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1750	0.11469611101534896	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1744	0.11430286720615349	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1727	0.11318867641343296	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1665	0.1091251570517463	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1651	0.1082075881636235	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	1594	0.10447177197626643	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1590	0.10420960943680277	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1588	0.10407852816707093	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1571	0.1029643373743504	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1566	0.10263663420002084	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.554063486591369E-5
2	0.0	0.0	0.0	0.0	6.554063486591369E-5
3	0.0	0.0	0.0	0.0	6.554063486591369E-5
4	0.0	0.0	0.0	0.0	6.554063486591369E-5
5	0.0	0.0	0.0	0.0	6.554063486591369E-5
6	0.0	0.0	0.0	0.0	6.554063486591369E-5
7	0.0	0.0	0.0	0.0	6.554063486591369E-5
8	0.0	0.0	0.0	0.0	6.554063486591369E-5
9	0.0	0.0	0.0	0.0	6.554063486591369E-5
10	0.0	0.0	0.0	0.0	6.554063486591369E-5
11	0.0	0.0	0.0	0.0	6.554063486591369E-5
12	0.0	0.0	0.0	0.0	1.9662190459774107E-4
13	0.0	0.0	0.0	0.0	2.6216253946365477E-4
14	0.0	0.0	0.0	0.0	2.6216253946365477E-4
15	0.0	0.0	0.0	0.0	2.6216253946365477E-4
16	0.0	0.0	0.0	1.3108126973182738E-4	2.6216253946365477E-4
17	0.0	0.0	0.0	1.9662190459774107E-4	2.6216253946365477E-4
18	0.0	0.0	0.0	3.2770317432956846E-4	3.2770317432956846E-4
19	0.0	0.0	0.0	3.2770317432956846E-4	3.2770317432956846E-4
20	0.0	0.0	0.0	4.5878444406139584E-4	3.9324380919548215E-4
21	0.0	0.0	0.0	6.554063486591369E-4	3.9324380919548215E-4
22	0.0	0.0	0.0	9.175688881227917E-4	3.9324380919548215E-4
23	0.0	0.0	0.0	0.0011797314275864466	3.9324380919548215E-4
24	0.0	0.0	0.0	0.0020317596808433246	3.9324380919548215E-4
25	6.554063486591369E-5	0.0	0.0	0.002293922220306979	4.5878444406139584E-4
26	6.554063486591369E-5	0.0	0.0	0.0030804098386979437	4.5878444406139584E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCTATA	125	0.0	20.492067	2
CCTATAC	85	2.4006113E-7	18.821041	3
GTATTAA	145	3.6379788E-12	17.676012	1
ACTAGAC	55	0.0013661323	17.45224	3
AGAACCG	400	0.0	17.197165	5
ATCGTTT	380	0.0	16.840984	29
AACCGCG	345	0.0	16.69454	7
CATCGTT	390	0.0	16.409163	28
CGGTCCT	365	0.0	16.219162	12
TCGTTTA	375	0.0	16.212255	30
GTATAGA	130	4.7293724E-9	16.018887	1
GATTAGA	80	5.5663466E-5	16.018885	1
AAGACGG	605	0.0	15.865151	5
CGCGGTC	365	0.0	15.780806	10
TACCGTC	400	0.0	15.598962	7
GTATCAA	3065	0.0	15.15653	1
ATACCGT	405	0.0	15.009871	6
ACCGTCG	375	0.0	14.93234	8
CTAAGAC	140	1.281478E-8	14.85518	3
GGTCCTA	400	0.0	14.799985	13
>>END_MODULE