FastQCFastQC Report
Thu 2 Feb 2017
SRR4062954_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062954_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1525771
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT34570.22657397473146365No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC25820.16922591922378916No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG25770.1688982160494596No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT24990.16378604652991832No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA24240.1588704989149748No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC23530.1542171138394949No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT23130.15159548844485837No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC22370.14661440019504893No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG22020.14432047797474196No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA21590.14150223067550766No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG21120.1384218208368097No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA20520.1344893827448549No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG19700.12911505068584997No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC19320.12662450656094526No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG19190.12577247830768837No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG19000.12452720624523601No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG18310.12000490243948797No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT17940.11757989894944916No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT16180.10604474721304835No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT16110.10558596276898696No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT16090.10545488149925512No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT15620.10237447166055719No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT15450.10126028086783664No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA15440.10119474023297073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCG5400.018.0754058
ATACCGT5400.017.7785056
TAGGACG450.00885114517.7779254
TACCGTC5500.017.7467637
TAGACCG652.1003136E-417.2314765
CGTCGTA5700.016.84279410
CGTTTAT4300.016.74377431
TCTAGCG5700.016.56099528
CCGTCGT5800.016.5529449
GAACTCG1651.8189894E-1216.48444431
CTAGCGG5750.016.41698829
ATCGTTT4200.016.3805529
TAGCGGC6050.015.60292230
AGATACC6100.015.4755364
GTATCAA31350.015.2610931
CAAGACG5700.015.1580184
CATCGTT4550.015.12050728
GTCGTAG6250.015.1046211
TAATACC859.43475E-515.0589474
GATATGC756.242518E-414.9344351
Produced by FastQC (version 0.11.5)