##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062954_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1525771
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.212823549536594	32.0	32.0	32.0	32.0	32.0
2	31.302439881214152	32.0	32.0	32.0	32.0	32.0
3	31.376731501647363	32.0	32.0	32.0	32.0	32.0
4	31.470878657413202	32.0	32.0	32.0	32.0	32.0
5	31.3983435259944	32.0	32.0	32.0	32.0	32.0
6	34.921444305862416	36.0	36.0	36.0	36.0	36.0
7	34.944905231519016	36.0	36.0	36.0	36.0	36.0
8	34.8793901575007	36.0	36.0	36.0	32.0	36.0
9	34.97988295753426	36.0	36.0	36.0	36.0	36.0
10	34.83433031562404	36.0	36.0	36.0	32.0	36.0
11	35.009393939195334	36.0	36.0	36.0	36.0	36.0
12	34.898757415103574	36.0	36.0	36.0	32.0	36.0
13	34.953519892565794	36.0	36.0	36.0	36.0	36.0
14	34.89557017403005	36.0	36.0	36.0	32.0	36.0
15	34.872227221516205	36.0	36.0	36.0	32.0	36.0
16	34.8848143004422	36.0	36.0	36.0	32.0	36.0
17	34.839996303508194	36.0	36.0	36.0	32.0	36.0
18	34.845174669068946	36.0	36.0	36.0	32.0	36.0
19	34.840962372466116	36.0	36.0	36.0	32.0	36.0
20	34.82647068269092	36.0	36.0	36.0	32.0	36.0
21	34.82061987021643	36.0	36.0	36.0	32.0	36.0
22	34.80262372269495	36.0	36.0	36.0	32.0	36.0
23	34.74387178678845	36.0	36.0	36.0	32.0	36.0
24	34.70851392509098	36.0	36.0	36.0	32.0	36.0
25	34.69545233196856	36.0	36.0	36.0	32.0	36.0
26	34.62534285944614	36.0	36.0	36.0	32.0	36.0
27	34.61619535303791	36.0	36.0	36.0	32.0	36.0
28	34.57923436741162	36.0	36.0	36.0	32.0	36.0
29	34.5460347588203	36.0	36.0	36.0	32.0	36.0
30	34.523722760492895	36.0	36.0	36.0	32.0	36.0
31	34.53474735068369	36.0	36.0	36.0	32.0	36.0
32	34.49877733945657	36.0	36.0	36.0	32.0	36.0
33	34.45866581551229	36.0	36.0	36.0	32.0	36.0
34	34.45402029531299	36.0	36.0	36.0	32.0	36.0
35	34.42450538121383	36.0	36.0	36.0	32.0	36.0
36	34.39450546641665	36.0	36.0	36.0	32.0	36.0
37	34.391908746463265	36.0	36.0	36.0	32.0	36.0
38	33.95527441536115	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	9.0
21	33.0
22	130.0
23	402.0
24	1067.0
25	2621.0
26	5554.0
27	10510.0
28	18216.0
29	28753.0
30	42842.0
31	61927.0
32	88497.0
33	140744.0
34	342243.0
35	782220.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.85598171995506	18.382234376290477	12.02682489096079	26.734959012793674
2	16.05129472247146	20.582118810752075	35.952970662045615	27.413615804730856
3	17.80162291720055	24.225850406122543	28.76624342709358	29.206283249583326
4	12.227260840584858	15.848774160735784	35.75333388824404	36.170631110435316
5	14.562810908590418	36.53558531102329	32.87625264620487	16.025351134181427
6	34.70193371469319	35.62127400758175	16.2096072273111	13.467185050413955
7	30.460674127846772	30.382156087048852	20.761467697494503	18.39570208760988
8	28.533002303141686	32.12659807490388	19.31368475141931	20.026714870535123
9	27.60618178171591	13.856115419126452	18.365822053877903	40.171880745279736
10	16.201782982622408	26.058391959930738	30.634281452235545	27.105543605211313
11	38.25980149582719	20.818752699561056	21.775749142530927	19.145696662080827
12	25.130754166437274	23.586282020024065	27.779066749510413	23.50389706402825
13	29.475543496070838	18.5931038098796	25.25531371045439	26.67603898359517
14	24.030146070412925	19.524948370364882	24.26071802387121	32.18418753535098
15	25.529781336779894	26.713641824362895	21.82050910654351	25.936067732313695
16	26.604337482058238	25.251905595207663	22.943300759616456	25.20045616311764
17	24.654682780050216	25.580706410070714	24.40510404248082	25.359506767398255
18	25.880096030138205	23.85620122547879	25.566549632939672	24.697153111443328
19	26.172603883544777	24.51508122778582	24.809162056429177	24.503152832240225
20	26.21055191113214	23.615404932981424	24.410937158983884	25.763105996902553
21	27.73755398097615	23.724102403443773	23.750777476800224	24.787566138779855
22	26.37594550682312	23.635622430394115	24.451801026236605	25.536631036546158
23	24.51239278343286	23.19023809008846	25.284594567846856	27.012774558631826
24	25.327850640758015	24.570135361073188	24.531335305232567	25.570678692936227
25	25.296260054752643	23.821333607730125	24.83636141989853	26.046044917618698
26	25.315348971339063	24.73708357091829	25.11918572261874	24.828381735123905
27	26.081471048369707	24.00199505954047	24.17069687482181	25.745837017268013
28	25.04130698512424	23.926460786055053	25.426948080675277	25.60528414814543
29	24.954531184561773	24.227751084533654	25.257787702086354	25.55993002881822
30	24.868869574792026	24.47208657131378	25.588243583080295	25.070800270813905
31	25.69625454933932	24.17872668965395	23.981908163151612	26.143110597855117
32	25.28334854968406	24.21837877374783	24.004191979006023	26.494080697562083
33	24.84435737735217	23.692218557044274	24.916976400783604	26.54644766481995
34	25.8430000308041	23.832475515657332	25.068637429863326	25.255887023675243
35	26.714952637060215	23.688023956412856	24.929298040138395	24.667725366388535
36	25.208697766571785	24.58455430074369	24.370564127906484	25.836183804778045
37	26.41248260715402	24.307710659070068	24.228734194056646	25.051072539719264
38	25.23701132083386	23.850761352784918	25.06935837684685	25.842868949534363
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	207.0
1	179.0
2	151.0
3	151.0
4	467.0
5	783.0
6	783.0
7	917.5
8	1052.0
9	967.0
10	882.0
11	882.0
12	1215.0
13	1548.0
14	2154.0
15	2760.0
16	2760.0
17	4264.0
18	5768.0
19	5768.0
20	6767.0
21	7766.0
22	8514.5
23	9263.0
24	9263.0
25	10823.5
26	12384.0
27	12384.0
28	15763.0
29	19142.0
30	23423.0
31	27704.0
32	27704.0
33	37653.5
34	47603.0
35	47603.0
36	53003.0
37	58403.0
38	67812.0
39	77221.0
40	77221.0
41	82632.0
42	88043.0
43	102165.5
44	116288.0
45	116288.0
46	119461.5
47	122635.0
48	122635.0
49	132658.0
50	142681.0
51	145945.0
52	149209.0
53	149209.0
54	142970.5
55	136732.0
56	136732.0
57	133577.0
58	130422.0
59	117236.5
60	104051.0
61	104051.0
62	100307.5
63	96564.0
64	79278.5
65	61993.0
66	61993.0
67	51925.0
68	41857.0
69	41857.0
70	33607.0
71	25357.0
72	19568.0
73	13779.0
74	13779.0
75	10279.0
76	6779.0
77	6779.0
78	6973.5
79	7168.0
80	5548.0
81	3928.0
82	3928.0
83	3895.0
84	3862.0
85	3862.0
86	2396.0
87	930.0
88	800.5
89	671.0
90	671.0
91	390.0
92	109.0
93	76.5
94	44.0
95	44.0
96	30.0
97	16.0
98	16.0
99	16.0
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.011338529831803069
2	0.0
3	0.0
4	0.0
5	6.554063486591369E-5
6	1.9662190459774107E-4
7	4.587844440613958E-4
8	0.0019006784111114969
9	0.004063519361686649
10	8.52028253256878E-4
11	0.003932438091954821
12	4.587844440613958E-4
13	6.554063486591369E-5
14	0.0
15	0.0
16	6.554063486591369E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	1.3108126973182738E-4
22	1.3108126973182738E-4
23	2.6216253946365477E-4
24	0.0
25	0.0
26	6.554063486591369E-5
27	1.3108126973182738E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1525771.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.82611818919897
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.82787843704494	38.97685427360164
2	11.83253135616858	11.554731489473797
3	3.393482923151011	4.97071794836399
4	1.4957470853534616	2.92126095882472
5	0.8013251072889728	1.9562797198231965
6	0.5273206000109263	1.544821076383968
7	0.3569178904168717	1.219884057093362
8	0.2741812065102232	1.0709763195460262
9	0.19499524590784834	0.8568774830725668
>10	1.0871171255739052	9.516196987527426
>50	0.085257899803826	2.987829492275157
>100	0.09919075420504063	10.839818083724296
>500	0.01635156514776694	5.632232355700055
>1k	0.007702803416716657	5.951519754589847
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3457	0.22657397473146365	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2582	0.16922591922378916	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2577	0.1688982160494596	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2499	0.16378604652991832	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2424	0.1588704989149748	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2353	0.1542171138394949	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2313	0.15159548844485837	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2237	0.14661440019504893	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2202	0.14432047797474196	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	2159	0.14150223067550766	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2112	0.1384218208368097	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2052	0.1344893827448549	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1970	0.12911505068584997	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1932	0.12662450656094526	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1919	0.12577247830768837	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1900	0.12452720624523601	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1831	0.12000490243948797	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1794	0.11757989894944916	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1618	0.10604474721304835	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1611	0.10558596276898696	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1609	0.10545488149925512	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1562	0.10237447166055719	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT	1545	0.10126028086783664	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1544	0.10119474023297073	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	1.3108126973182738E-4
13	0.0	0.0	0.0	0.0	1.9662190459774107E-4
14	0.0	0.0	0.0	0.0	1.9662190459774107E-4
15	0.0	0.0	0.0	6.554063486591369E-5	3.2770317432956846E-4
16	0.0	0.0	0.0	1.9662190459774107E-4	3.2770317432956846E-4
17	0.0	0.0	0.0	2.6216253946365477E-4	3.2770317432956846E-4
18	0.0	0.0	0.0	3.9324380919548215E-4	3.9324380919548215E-4
19	0.0	0.0	0.0	3.9324380919548215E-4	3.9324380919548215E-4
20	0.0	0.0	0.0	4.5878444406139584E-4	5.898657137932233E-4
21	0.0	0.0	0.0	6.554063486591369E-4	5.898657137932233E-4
22	0.0	0.0	0.0	9.831095229887053E-4	5.898657137932233E-4
23	0.0	0.0	0.0	0.00124527206245236	5.898657137932233E-4
24	6.554063486591369E-5	0.0	0.0	0.0020317596808433246	6.554063486591369E-4
25	6.554063486591369E-5	0.0	0.0	0.002359462855172893	6.554063486591369E-4
26	6.554063486591369E-5	0.0	0.0	0.003145950473563857	6.554063486591369E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTCG	540	0.0	18.075405	8
ATACCGT	540	0.0	17.778505	6
TAGGACG	45	0.008851145	17.777925	4
TACCGTC	550	0.0	17.746763	7
TAGACCG	65	2.1003136E-4	17.231476	5
CGTCGTA	570	0.0	16.842794	10
CGTTTAT	430	0.0	16.743774	31
TCTAGCG	570	0.0	16.560995	28
CCGTCGT	580	0.0	16.552944	9
GAACTCG	165	1.8189894E-12	16.484444	31
CTAGCGG	575	0.0	16.416988	29
ATCGTTT	420	0.0	16.38055	29
TAGCGGC	605	0.0	15.602922	30
AGATACC	610	0.0	15.475536	4
GTATCAA	3135	0.0	15.261093	1
CAAGACG	570	0.0	15.158018	4
CATCGTT	455	0.0	15.120507	28
GTCGTAG	625	0.0	15.10462	11
TAATACC	85	9.43475E-5	15.058947	4
GATATGC	75	6.242518E-4	14.934435	1
>>END_MODULE