FastQCFastQC Report
Thu 2 Feb 2017
SRR4062953_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062953_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1478470
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC34700.23470209067481926No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT32930.22273025492570023No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT30140.20385939518556348No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT30120.20372412020534741No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA28050.1897231597529879No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC26380.17842769890494903No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC26000.1758574742808444No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG25490.1724079622853355No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG24450.16537366331410172No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC24280.16422382598226545No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG23800.16097722645708062No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG22490.1521167152529304No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG22330.15103451541120214No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC21800.1474497284354772No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG21300.14406785393007637No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA21040.1423092791872679No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC20920.1414976293059717No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA20430.13818339229067889No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG20050.13561316766657422No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT18380.12431770681853536No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG18340.12404715685810332No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT17730.11992126996151425No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC17600.11904198259011003No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT16950.11464554573308892No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG16770.11342807091114462No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT16680.11281933350017247No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT16530.11180477114855222No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC16330.11045202134639187No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT16320.11038438385628387No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA16070.10869344660358343No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG16010.10828762166293533No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT15940.10781415923217923No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG15350.1038235473158062No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG15190.10274134747407793No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC15130.10233552253342981No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT14890.10071222277083741No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG6150.020.29199628
TAGCGGC6000.020.2659830
CTAGCGG6000.020.2659829
ATCGTTT4450.019.77461229
CGTTTAT4750.018.8625231
CGCTTAC1208.367351E-1118.66477218
CCTTATA953.77986E-818.531332
AGCGGCG6650.018.52568831
CATCGTT4800.018.33271228
GCATCGT5200.017.84554927
GGCGTAT450.00885316417.77717829
TCGTTTA4950.017.77717630
TAGGACG7200.017.773574
GGCATCG5400.017.4808926
TAGATCG550.001367373417.4498215
GTCCTAC9750.017.4125421
GCGGCGC7250.017.2132132
CGGTCCA7650.017.15090810
GTCCTAA4250.016.9583851
ACCTCTA7400.016.86486425
Produced by FastQC (version 0.11.5)