##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062953_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1478470
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.255267945917062	32.0	32.0	32.0	32.0	32.0
2	30.82856669394712	32.0	32.0	32.0	32.0	32.0
3	30.879214322914905	32.0	32.0	32.0	32.0	32.0
4	30.9537264875175	32.0	32.0	32.0	32.0	32.0
5	30.833039561167965	32.0	32.0	32.0	32.0	32.0
6	34.509789850318235	36.0	36.0	36.0	32.0	36.0
7	34.43384444730025	36.0	36.0	36.0	32.0	36.0
8	34.408938970692674	36.0	36.0	36.0	32.0	36.0
9	34.57562615406467	36.0	36.0	36.0	32.0	36.0
10	34.24315407143872	36.0	36.0	36.0	32.0	36.0
11	34.549081144696885	36.0	36.0	36.0	32.0	36.0
12	34.33561519679128	36.0	36.0	36.0	32.0	36.0
13	34.45558246024606	36.0	36.0	36.0	32.0	36.0
14	34.330547796032384	36.0	36.0	36.0	32.0	36.0
15	34.25134226599119	36.0	36.0	36.0	32.0	36.0
16	34.27807666033129	36.0	36.0	36.0	32.0	36.0
17	34.18425399230286	36.0	36.0	36.0	32.0	36.0
18	34.232233998660774	36.0	36.0	36.0	32.0	36.0
19	34.209519300357805	36.0	36.0	36.0	32.0	36.0
20	34.19329509560559	36.0	36.0	36.0	32.0	36.0
21	34.166566112264704	36.0	36.0	36.0	32.0	36.0
22	34.14015705425203	36.0	36.0	36.0	32.0	36.0
23	34.08022685614182	36.0	36.0	36.0	32.0	36.0
24	34.05891157750918	36.0	36.0	36.0	32.0	36.0
25	34.039209453015616	36.0	36.0	36.0	32.0	36.0
26	33.98277611314399	36.0	36.0	36.0	32.0	36.0
27	33.97811183182615	36.0	36.0	36.0	32.0	36.0
28	33.96857697484562	36.0	36.0	36.0	32.0	36.0
29	33.92812231563711	36.0	36.0	36.0	32.0	36.0
30	33.89053819150879	36.0	36.0	36.0	32.0	36.0
31	33.914249190041055	36.0	36.0	36.0	32.0	36.0
32	33.86289204380204	36.0	36.0	36.0	32.0	36.0
33	33.830853517487675	36.0	36.0	36.0	32.0	36.0
34	33.841373176324176	36.0	36.0	36.0	32.0	36.0
35	33.79875208830751	36.0	36.0	36.0	32.0	36.0
36	33.747118304733945	36.0	36.0	36.0	32.0	36.0
37	33.74780076700914	36.0	36.0	36.0	32.0	36.0
38	33.11084634791372	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	2.0
8	6.0
9	8.0
10	10.0
11	13.0
12	11.0
13	11.0
14	116.0
15	323.0
16	384.0
17	477.0
18	629.0
19	848.0
20	1232.0
21	1730.0
22	2807.0
23	4486.0
24	6695.0
25	10093.0
26	14969.0
27	21557.0
28	30662.0
29	40895.0
30	54950.0
31	73500.0
32	101769.0
33	152438.0
34	332357.0
35	625490.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.21457823010851	16.895311455581375	11.199249085228962	25.69086122908115
2	17.039412197431172	18.847769989828368	37.90885324494007	26.20396456780039
3	19.83678207416918	22.908347533500333	27.69874765688789	29.556122735442603
4	12.341512082114413	14.535739046620558	35.815546950301844	37.30720192096319
5	14.806735886920864	36.34455642965798	33.17918738911671	15.669520294304446
6	35.44356109643373	35.30382166380559	16.146465322841376	13.106151916919293
7	31.373759864941274	29.6496779098364	19.945105338769594	19.031456886452734
8	28.682402818059828	32.985652677373274	18.71699277087572	19.614951733691182
9	27.540591086025035	13.66099298935044	18.2410811212981	40.55733480332642
10	16.447903294098868	25.518139796626492	30.36655659732885	27.667400311945794
11	39.307090683176035	20.32293651453938	21.61004252475425	18.75993027753033
12	25.82601472919812	22.97622332970785	27.982094446518722	23.215667494575303
13	29.935633567956817	18.398923054547424	25.216894243550676	26.448549133945093
14	23.64784834769527	20.048040727064137	23.84834498951181	32.455765935728785
15	25.282979807307846	28.003888752453303	21.454439422261824	25.258692017977026
16	26.416317054791094	25.142764759122944	23.906552499956028	24.534365686129934
17	24.249113589729888	25.917682405001376	24.752444435724854	25.080759569543883
18	25.785131457815737	23.811082784366434	26.570719495556972	23.833066262260864
19	26.289248360663496	24.133454910974592	24.621569298579086	24.955727429782822
20	26.378824374534542	23.58556229491896	24.44102628622295	25.594587044323546
21	28.008704186843747	23.42351605045831	23.45368443196523	25.114095330732724
22	26.693800300602966	24.02868850795678	24.600402337181286	24.677108854258968
23	24.428459429531586	23.753846403608396	25.520300284983925	26.29739388187609
24	25.36485808180776	24.51554071514484	25.003619154051492	25.115982048995907
25	25.15513685123436	24.27359828874227	25.062321955390672	25.508942904632693
26	24.36665358751979	25.917209000257074	25.754103018576895	23.96203439364624
27	25.652573597801315	24.297201115576332	24.8707502135843	25.17947507303805
28	24.390243902439025	24.682388401342553	25.444507426382156	25.482860269836266
29	24.317320258785138	24.37454846539114	25.45884033736231	25.84929093846141
30	24.037724005730436	25.01207368396845	25.961802516466882	24.988399793834237
31	25.836427957818408	24.653368236639	23.915663873049372	25.59453993249322
32	25.162019985565152	24.626090815068245	24.05004981848215	26.161839380884448
33	24.345853248715787	24.1943993863379	25.39074284771142	26.069004517234895
34	25.77048585931835	24.604813135194227	25.19407584990205	24.430625155585382
35	26.249950109556032	24.243846901617395	25.47937106246013	24.02683192636644
36	24.031597183935144	25.350536755707658	24.9952477045229	25.622618355834298
37	25.89136474234656	24.882721840896746	24.432684873332825	24.793228543423865
38	24.70487966557989	24.715431756381985	24.891502861240006	25.688185716798117
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	48.0
1	83.0
2	118.0
3	118.0
4	301.5
5	485.0
6	485.0
7	625.5
8	766.0
9	783.5
10	801.0
11	801.0
12	1062.0
13	1323.0
14	1803.0
15	2283.0
16	2283.0
17	3334.5
18	4386.0
19	4386.0
20	5639.0
21	6892.0
22	7611.0
23	8330.0
24	8330.0
25	11002.0
26	13674.0
27	13674.0
28	18458.5
29	23243.0
30	29784.0
31	36325.0
32	36325.0
33	47034.0
34	57743.0
35	57743.0
36	62776.0
37	67809.0
38	76516.5
39	85224.0
40	85224.0
41	85913.0
42	86602.0
43	97797.5
44	108993.0
45	108993.0
46	108039.5
47	107086.0
48	107086.0
49	115571.5
50	124057.0
51	128925.0
52	133793.0
53	133793.0
54	127514.0
55	121235.0
56	121235.0
57	119096.0
58	116957.0
59	105325.0
60	93693.0
61	93693.0
62	93084.5
63	92476.0
64	78158.0
65	63840.0
66	63840.0
67	54550.0
68	45260.0
69	45260.0
70	36667.0
71	28074.0
72	22061.0
73	16048.0
74	16048.0
75	12252.5
76	8457.0
77	8457.0
78	8202.5
79	7948.0
80	6285.0
81	4622.0
82	4622.0
83	4694.5
84	4767.0
85	4767.0
86	3223.5
87	1680.0
88	1453.0
89	1226.0
90	1226.0
91	907.5
92	589.0
93	487.0
94	385.0
95	385.0
96	371.0
97	357.0
98	357.0
99	616.0
100	875.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12654974399209992
2	0.05620675427976218
3	0.01359513551171143
4	0.0030436870548607685
5	6.763749010801707E-5
6	2.7054996043206827E-4
7	1.3527498021603413E-4
8	4.0582494064810243E-4
9	3.381874505400854E-4
10	5.410999208641365E-4
11	0.0025025871339966316
12	0.0031113245449687858
13	0.01454206037322367
14	0.009536886105230407
15	0.024417133928994163
16	0.0110925483777148
17	0.021441084364241414
18	0.005884461639397485
19	0.008657598733826185
20	0.006155011599829554
21	0.0068313865009097235
22	0.0073048489316658435
23	0.009536886105230407
24	0.015150797784195823
25	0.017518109937976423
26	0.019614872131324953
27	0.008860511204150236
28	0.0062226490899375715
29	0.01203947323922704
30	0.0032465995251848194
31	0.006763749010801707
32	0.006966661481125759
33	0.008928148694258254
34	0.012310023199659108
35	0.015624260214951944
36	0.016030085155600046
37	0.00994271104587851
38	0.006087374109721537
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1478470.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.46806623163274
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.82050254289209	34.474116313358955
2	13.919930610488876	12.658246538748802
3	4.689045795556882	6.396055343866179
4	2.01122124024159	3.6578536223108458
5	1.0364433978163299	2.3562538528625683
6	0.5777715811151232	1.5762093910138553
7	0.3806091781328218	1.2113894323797312
8	0.24398873146427327	0.8874956641591707
9	0.17832605901489998	0.7297326955903968
>10	0.905859768473149	7.57264517866514
>50	0.09385271815287527	3.0380083047709077
>100	0.11109184767727183	10.9503570765608
>500	0.02135230306319817	6.768914706603919
>1k	0.010004225910729213	7.722721879108768
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3470	0.23470209067481926	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3293	0.22273025492570023	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	3014	0.20385939518556348	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3012	0.20372412020534741	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2805	0.1897231597529879	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2638	0.17842769890494903	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2600	0.1758574742808444	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2549	0.1724079622853355	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2445	0.16537366331410172	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	2428	0.16422382598226545	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2380	0.16097722645708062	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2249	0.1521167152529304	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	2233	0.15103451541120214	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2180	0.1474497284354772	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2130	0.14406785393007637	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2104	0.1423092791872679	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	2092	0.1414976293059717	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	2043	0.13818339229067889	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2005	0.13561316766657422	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1838	0.12431770681853536	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	1834	0.12404715685810332	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1773	0.11992126996151425	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	1760	0.11904198259011003	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT	1695	0.11464554573308892	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1677	0.11342807091114462	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1668	0.11281933350017247	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	1653	0.11180477114855222	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	1633	0.11045202134639187	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1632	0.11038438385628387	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1607	0.10869344660358343	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG	1601	0.10828762166293533	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1594	0.10781415923217923	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG	1535	0.1038235473158062	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	1519	0.10274134747407793	No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC	1513	0.10233552253342981	No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT	1489	0.10071222277083741	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	6.763749010801707E-5	0.0
8	0.0	0.0	0.0	6.763749010801707E-5	0.0
9	0.0	0.0	0.0	6.763749010801707E-5	0.0
10	0.0	0.0	0.0	6.763749010801707E-5	0.0
11	0.0	0.0	0.0	6.763749010801707E-5	0.0
12	0.0	0.0	0.0	6.763749010801707E-5	0.0
13	0.0	0.0	0.0	6.763749010801707E-5	0.0
14	0.0	0.0	0.0	6.763749010801707E-5	0.0
15	0.0	0.0	0.0	6.763749010801707E-5	0.0
16	0.0	0.0	0.0	6.763749010801707E-5	0.0
17	0.0	0.0	0.0	1.3527498021603413E-4	0.0
18	0.0	0.0	0.0	2.7054996043206827E-4	0.0
19	0.0	0.0	0.0	3.381874505400854E-4	0.0
20	0.0	0.0	0.0	3.381874505400854E-4	6.763749010801707E-5
21	0.0	0.0	0.0	5.410999208641365E-4	6.763749010801707E-5
22	0.0	0.0	0.0	7.440123911881878E-4	6.763749010801707E-5
23	0.0	0.0	0.0	0.001014562351620256	6.763749010801707E-5
24	0.0	0.0	0.0	0.0016232997625924097	6.763749010801707E-5
25	0.0	0.0	0.0	0.001893849723024478	6.763749010801707E-5
26	0.0	0.0	0.0	0.002570224624104649	6.763749010801707E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	615	0.0	20.291996	28
TAGCGGC	600	0.0	20.26598	30
CTAGCGG	600	0.0	20.26598	29
ATCGTTT	445	0.0	19.774612	29
CGTTTAT	475	0.0	18.86252	31
CGCTTAC	120	8.367351E-11	18.664772	18
CCTTATA	95	3.77986E-8	18.53133	2
AGCGGCG	665	0.0	18.525688	31
CATCGTT	480	0.0	18.332712	28
GCATCGT	520	0.0	17.845549	27
GGCGTAT	45	0.008853164	17.777178	29
TCGTTTA	495	0.0	17.777176	30
TAGGACG	720	0.0	17.77357	4
GGCATCG	540	0.0	17.48089	26
TAGATCG	55	0.0013673734	17.449821	5
GTCCTAC	975	0.0	17.412542	1
GCGGCGC	725	0.0	17.21321	32
CGGTCCA	765	0.0	17.150908	10
GTCCTAA	425	0.0	16.958385	1
ACCTCTA	740	0.0	16.864864	25
>>END_MODULE