FastQCFastQC Report
Thu 2 Feb 2017
SRR4062953_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062953_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1478470
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC37260.2520172881424716No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT33240.22482701711904873No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT32060.2168457932863027No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT30470.20609143235912802No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA29020.19628399629346552No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC28360.1918199219463364No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC26980.18248594831143006No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG25490.1724079622853355No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC24420.1651707508437777No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG24420.1651707508437777No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG24210.16375036355150932No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG23760.16070667649664858No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC22720.1536723775254148No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG21800.1474497284354772No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT21690.14670571604428903No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG21540.14569115369266877No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG21110.14278274161802404No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA21060.14244455416748397No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG20610.13940086711262317No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA19660.13297530555236156No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA19600.13256948061171345No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC19470.13169019324030923No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG19370.13101381833922907No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT19010.12857886869534044No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT18720.12661738148220797No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC18460.12485880673939953No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG17620.11917725757032609No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT17440.11795978274838177No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT16880.11417208330233282No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC16870.1141044458122248No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCT16310.11031674636617585No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC16290.11018147138595982No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT16050.10855817162336741No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC15680.10605558448937076No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGC15630.10571739703883068No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG15580.1053792095882906No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC15530.10504102213775052No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG15480.10470283468721044No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC15470.10463519719710242No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGG15250.10314717241472604No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG15100.10213261006310578No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGTT4350.022.06810228
ATCGTTT4650.020.98842629
TCTAGCG6500.020.4299728
CTAGCGG6550.020.27401529
TAGCGGC6500.020.18382630
TACACCG400.004480703720.00265
GCATCGT4900.019.5910727
CGGCATC4950.019.3931825
TCGTTTA5150.018.64004730
GGCATCG5150.018.64004726
GGAGCGT1301.2732926E-1118.4639386
CGTTTAT5200.018.46081731
GTATAAT1055.979018E-918.2862361
GTAGGAC10600.018.2652913
AGCGGCG7350.018.0673231
GTATTAC904.367521E-717.7782841
TGTAGGA11700.017.6409322
CGGTCCA8000.017.60050210
GATAGTC652.0988297E-417.233017
CGCGAAC652.1015437E-417.23009512
Produced by FastQC (version 0.11.5)