##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062953_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1478470
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.186145136526275	32.0	32.0	32.0	32.0	32.0
2	31.254243914316827	32.0	32.0	32.0	32.0	32.0
3	31.352841112771987	32.0	32.0	32.0	32.0	32.0
4	31.45794165590103	32.0	32.0	32.0	32.0	32.0
5	31.363626586944612	32.0	32.0	32.0	32.0	32.0
6	34.90819157642698	36.0	36.0	36.0	36.0	36.0
7	34.93244840950442	36.0	36.0	36.0	36.0	36.0
8	34.85833395334366	36.0	36.0	36.0	32.0	36.0
9	34.97587506002827	36.0	36.0	36.0	36.0	36.0
10	34.8053832678377	36.0	36.0	36.0	32.0	36.0
11	35.00543805420468	36.0	36.0	36.0	36.0	36.0
12	34.88607276441186	36.0	36.0	36.0	32.0	36.0
13	34.956520592233865	36.0	36.0	36.0	36.0	36.0
14	34.88558577448308	36.0	36.0	36.0	32.0	36.0
15	34.85124689713014	36.0	36.0	36.0	32.0	36.0
16	34.86404188113387	36.0	36.0	36.0	32.0	36.0
17	34.819238807686325	36.0	36.0	36.0	32.0	36.0
18	34.84175397539348	36.0	36.0	36.0	32.0	36.0
19	34.82633533314846	36.0	36.0	36.0	32.0	36.0
20	34.811576832806885	36.0	36.0	36.0	32.0	36.0
21	34.79712946491981	36.0	36.0	36.0	32.0	36.0
22	34.78566626309631	36.0	36.0	36.0	32.0	36.0
23	34.719644632626974	36.0	36.0	36.0	32.0	36.0
24	34.69158386710586	36.0	36.0	36.0	32.0	36.0
25	34.66460259592687	36.0	36.0	36.0	32.0	36.0
26	34.591129343172334	36.0	36.0	36.0	32.0	36.0
27	34.58493848370275	36.0	36.0	36.0	32.0	36.0
28	34.541112095612355	36.0	36.0	36.0	32.0	36.0
29	34.497174782038186	36.0	36.0	36.0	32.0	36.0
30	34.47724742470256	36.0	36.0	36.0	32.0	36.0
31	34.47805501633445	36.0	36.0	36.0	32.0	36.0
32	34.44768848877556	36.0	36.0	36.0	32.0	36.0
33	34.42091892294061	36.0	36.0	36.0	32.0	36.0
34	34.41387583109566	36.0	36.0	36.0	32.0	36.0
35	34.36635373054577	36.0	36.0	36.0	32.0	36.0
36	34.33868729159198	36.0	36.0	36.0	32.0	36.0
37	34.33429694210907	36.0	36.0	36.0	32.0	36.0
38	33.85937759981603	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	5.0
21	28.0
22	117.0
23	320.0
24	1018.0
25	2439.0
26	5144.0
27	10096.0
28	17458.0
29	27729.0
30	42105.0
31	61405.0
32	88661.0
33	144493.0
34	351268.0
35	726183.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.434365982247584	18.27455771158615	11.88134751686696	26.409728789299308
2	15.953857704248312	19.909433400745367	37.14664484230319	26.990064052703133
3	18.67856635575967	23.6218523203041	28.11054671383254	29.58903461010369
4	11.75130963533124	15.410713138288697	35.92049862526336	36.9174786011167
5	14.301879645850102	36.8767036192821	32.80249176513558	16.018924969732222
6	35.414675586704895	35.72912249090106	15.76096624278223	13.095235679611813
7	31.099797560033632	29.92398186495029	19.949839799846192	19.02638077516989
8	28.574337052356043	33.225403889924145	18.446465350184486	19.753793707535333
9	27.27977408390686	13.584794629419822	18.00706833285422	41.1283629538191
10	16.189095080530176	25.894516697194092	30.182177033816448	27.73421118845928
11	39.36416525128805	20.250973516693353	21.51294335847533	18.871917873543275
12	25.588802455516422	23.090877239735484	27.85391895794766	23.466401346800435
13	29.95102359403355	18.422392924563077	25.209355366064983	26.417228115338386
14	23.492326526747245	20.069801889791474	23.783911746602907	32.65395983685838
15	25.257191556135734	28.06976130729741	21.243447618145787	25.42959951842107
16	26.445464869402063	25.303269801395906	23.670702598431216	24.580562730770815
17	24.310537244583927	26.181187308501357	24.40245659364072	25.10581885327399
18	25.78192320439373	23.862844697558963	26.47710132772393	23.878130770323374
19	26.28676943055997	24.160449654034238	24.557211171007868	24.995569744397926
20	26.469931753772478	23.669469113340142	24.260823689354535	25.59977544353284
21	27.854219466218094	23.59454273305697	23.421931741551564	25.129306059173373
22	26.480956664660088	24.12588689658904	24.56695096958342	24.82620546916745
23	24.37863002691301	23.711993571719898	25.573381076479894	26.335995324887197
24	25.315765622569273	24.509459102991606	25.015860991430326	25.158914283008787
25	25.044877474686672	24.340433015211673	25.062057397174105	25.552632112927554
26	24.457800603191547	25.879135781676855	25.634896639699583	24.028166975432015
27	25.61849537392448	24.238823051173952	24.779450606608062	25.36323096829351
28	24.280303286505646	24.71548289786063	25.57197643509845	25.432237380535284
29	24.333601628710763	24.246552178941744	25.4475910907898	25.97225510155769
30	23.92885888790439	24.897021921310543	26.074861174051556	25.099258016733515
31	25.789566240775937	24.53549953668319	23.97065885679114	25.70427536574973
32	25.182519767056483	24.606045438865852	24.145975231151123	26.06545956292654
33	24.285376098263743	24.099643550427132	25.554525962650576	26.060454388658545
34	25.669306783363883	24.47252903339263	25.35256041718804	24.50560376605545
35	26.226572064363836	24.21428909616022	25.388137736984856	24.17100110249109
36	24.071100529601548	25.327128720907428	24.99408171961555	25.60768902987548
37	26.019871894593738	24.832833943198036	24.304314595493988	24.842979566714238
38	24.81742938133975	24.47126047282696	24.991528398633992	25.7197817471993
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	26.0
1	34.0
2	42.0
3	42.0
4	172.0
5	302.0
6	302.0
7	448.0
8	594.0
9	704.5
10	815.0
11	815.0
12	1017.0
13	1219.0
14	1707.5
15	2196.0
16	2196.0
17	3387.5
18	4579.0
19	4579.0
20	5709.5
21	6840.0
22	7559.5
23	8279.0
24	8279.0
25	10952.5
26	13626.0
27	13626.0
28	18402.5
29	23179.0
30	29637.0
31	36095.0
32	36095.0
33	47505.0
34	58915.0
35	58915.0
36	63433.5
37	67952.0
38	76655.0
39	85358.0
40	85358.0
41	86280.0
42	87202.0
43	98392.0
44	109582.0
45	109582.0
46	108272.0
47	106962.0
48	106962.0
49	115644.5
50	124327.0
51	128803.0
52	133279.0
53	133279.0
54	127650.5
55	122022.0
56	122022.0
57	119762.0
58	117502.0
59	105798.0
60	94094.0
61	94094.0
62	93666.5
63	93239.0
64	78301.0
65	63363.0
66	63363.0
67	54292.0
68	45221.0
69	45221.0
70	36935.0
71	28649.0
72	22353.0
73	16057.0
74	16057.0
75	12068.5
76	8080.0
77	8080.0
78	8080.0
79	8080.0
80	6369.5
81	4659.0
82	4659.0
83	4258.0
84	3857.0
85	3857.0
86	2563.5
87	1270.0
88	1008.0
89	746.0
90	746.0
91	451.5
92	157.0
93	102.5
94	48.0
95	48.0
96	42.5
97	37.0
98	37.0
99	28.5
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009198698654690323
2	0.0
3	0.0
4	3.381874505400854E-4
5	0.0
6	6.763749010801707E-5
7	4.734624307561195E-4
8	0.0023673121537805975
9	0.0030436870548607685
10	0.0012174748219443072
11	0.004531711837237144
12	2.0291247032405121E-4
13	2.0291247032405121E-4
14	0.0
15	0.0
16	6.763749010801707E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	6.763749010801707E-5
22	0.0
23	2.0291247032405121E-4
24	0.0
25	0.0
26	6.763749010801707E-5
27	2.0291247032405121E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	6.763749010801707E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1478470.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.11858686771496
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.5711332103352	31.722832982860677
2	15.306487893332818	13.199882557365969
3	5.044604820398784	6.525486934849758
4	2.2201271602820682	3.8291498327198283
5	1.1066337493269423	2.3858241725549427
6	0.6225341980222685	1.610567693732787
7	0.4138893539113362	1.2492426842173858
8	0.267442830991869	0.922540555221641
9	0.19431555171298012	0.7540750796655682
>10	0.9924463642346297	7.778390865506349
>50	0.10508962528463077	3.169728270196168
>100	0.12092535183088275	11.356330467767243
>500	0.02223006668498749	6.705556602167685
>1k	0.01213982365066693	8.790391301173948
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3726	0.2520172881424716	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3324	0.22482701711904873	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3206	0.2168457932863027	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	3047	0.20609143235912802	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2902	0.19628399629346552	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2836	0.1918199219463364	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2698	0.18248594831143006	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2549	0.1724079622853355	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2442	0.1651707508437777	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2442	0.1651707508437777	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2421	0.16375036355150932	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2376	0.16070667649664858	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	2272	0.1536723775254148	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	2180	0.1474497284354772	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2169	0.14670571604428903	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2154	0.14569115369266877	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2111	0.14278274161802404	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2106	0.14244455416748397	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	2061	0.13940086711262317	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1966	0.13297530555236156	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1960	0.13256948061171345	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1947	0.13169019324030923	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1937	0.13101381833922907	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1901	0.12857886869534044	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT	1872	0.12661738148220797	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	1846	0.12485880673939953	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG	1762	0.11917725757032609	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1744	0.11795978274838177	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1688	0.11417208330233282	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC	1687	0.1141044458122248	No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCT	1631	0.11031674636617585	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	1629	0.11018147138595982	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1605	0.10855817162336741	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	1568	0.10605558448937076	No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGC	1563	0.10571739703883068	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	1558	0.1053792095882906	No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC	1553	0.10504102213775052	No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG	1548	0.10470283468721044	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC	1547	0.10463519719710242	No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGG	1525	0.10314717241472604	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG	1510	0.10213261006310578	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	6.763749010801707E-5
13	0.0	0.0	0.0	0.0	6.763749010801707E-5
14	0.0	0.0	0.0	0.0	2.0291247032405121E-4
15	0.0	0.0	0.0	0.0	2.0291247032405121E-4
16	0.0	0.0	0.0	0.0	2.0291247032405121E-4
17	0.0	0.0	0.0	6.763749010801707E-5	2.0291247032405121E-4
18	0.0	0.0	0.0	6.763749010801707E-5	2.0291247032405121E-4
19	0.0	0.0	0.0	1.3527498021603413E-4	2.7054996043206827E-4
20	0.0	0.0	0.0	2.0291247032405121E-4	3.381874505400854E-4
21	0.0	0.0	0.0	4.734624307561195E-4	3.381874505400854E-4
22	0.0	0.0	0.0	7.440123911881878E-4	3.381874505400854E-4
23	0.0	0.0	0.0	9.46924861512239E-4	4.0582494064810243E-4
24	0.0	0.0	0.0	0.0017585747428084438	4.0582494064810243E-4
25	0.0	0.0	0.0	0.002029124703240512	4.0582494064810243E-4
26	0.0	0.0	0.0	0.0025025871339966316	4.0582494064810243E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATCGTT	435	0.0	22.068102	28
ATCGTTT	465	0.0	20.988426	29
TCTAGCG	650	0.0	20.42997	28
CTAGCGG	655	0.0	20.274015	29
TAGCGGC	650	0.0	20.183826	30
TACACCG	40	0.0044807037	20.0026	5
GCATCGT	490	0.0	19.59107	27
CGGCATC	495	0.0	19.39318	25
TCGTTTA	515	0.0	18.640047	30
GGCATCG	515	0.0	18.640047	26
GGAGCGT	130	1.2732926E-11	18.463938	6
CGTTTAT	520	0.0	18.460817	31
GTATAAT	105	5.979018E-9	18.286236	1
GTAGGAC	1060	0.0	18.265291	3
AGCGGCG	735	0.0	18.06732	31
GTATTAC	90	4.367521E-7	17.778284	1
TGTAGGA	1170	0.0	17.640932	2
CGGTCCA	800	0.0	17.600502	10
GATAGTC	65	2.0988297E-4	17.23301	7
CGCGAAC	65	2.1015437E-4	17.230095	12
>>END_MODULE