FastQCFastQC Report
Thu 2 Feb 2017
SRR4062952_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062952_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2380725
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT31610.13277467998193826No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT28710.12059351668084302No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT28140.11819928803200705No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC25900.10879038948219555No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30150.019.9174181
GGTATCA11150.019.1014271
CTAGCGG5250.017.98074229
CGTTCGG450.00885036917.77868814
GTAGGAC17450.017.0517313
TGTAGGA17650.016.9552022
TCTAGCG5600.016.85623628
TAGCGGC5700.016.84155330
GTCCTAA9000.016.547391
TAGGACG14750.016.2675764
TATACTG2950.016.2675765
AACCGCG2400.015.9977957
TACCGTC4650.015.8257747
ATACCGT4800.015.6631936
ACCGTCG4400.015.6345378
GTCCTAC15300.015.4902831
CTGTAGG18150.015.4401131
CGCGGTC2500.015.36078610
GTGTATA5350.015.2653051
TTAGGAC9200.015.1280313
Produced by FastQC (version 0.11.5)