##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062952_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2380725
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.305961839355657	32.0	32.0	32.0	32.0	32.0
2	30.91004294910164	32.0	32.0	32.0	32.0	32.0
3	30.949862751892805	32.0	32.0	32.0	32.0	32.0
4	31.026402461435065	32.0	32.0	32.0	32.0	32.0
5	30.919302313370927	32.0	32.0	32.0	32.0	32.0
6	34.60301756817776	36.0	36.0	36.0	32.0	36.0
7	34.5489273225593	36.0	36.0	36.0	32.0	36.0
8	34.524047506536874	36.0	36.0	36.0	32.0	36.0
9	34.67820978903485	36.0	36.0	36.0	32.0	36.0
10	34.37729599176721	36.0	36.0	36.0	32.0	36.0
11	34.65645801174012	36.0	36.0	36.0	32.0	36.0
12	34.46765544109463	36.0	36.0	36.0	32.0	36.0
13	34.554408426004684	36.0	36.0	36.0	32.0	36.0
14	34.45057703010637	36.0	36.0	36.0	32.0	36.0
15	34.38164466706571	36.0	36.0	36.0	32.0	36.0
16	34.412513415031135	36.0	36.0	36.0	32.0	36.0
17	34.345250711442944	36.0	36.0	36.0	32.0	36.0
18	34.36900313979985	36.0	36.0	36.0	32.0	36.0
19	34.3587701225467	36.0	36.0	36.0	32.0	36.0
20	34.340793245754966	36.0	36.0	36.0	32.0	36.0
21	34.32978735469237	36.0	36.0	36.0	32.0	36.0
22	34.30336935177309	36.0	36.0	36.0	32.0	36.0
23	34.24842306440265	36.0	36.0	36.0	32.0	36.0
24	34.229354923395185	36.0	36.0	36.0	32.0	36.0
25	34.20898717827553	36.0	36.0	36.0	32.0	36.0
26	34.156015919520314	36.0	36.0	36.0	32.0	36.0
27	34.16061031828539	36.0	36.0	36.0	32.0	36.0
28	34.14070125697004	36.0	36.0	36.0	32.0	36.0
29	34.10441273141585	36.0	36.0	36.0	32.0	36.0
30	34.082078778523346	36.0	36.0	36.0	32.0	36.0
31	34.09688225225509	36.0	36.0	36.0	32.0	36.0
32	34.054547249262306	36.0	36.0	36.0	32.0	36.0
33	34.03759233006752	36.0	36.0	36.0	32.0	36.0
34	34.04314190005145	36.0	36.0	36.0	32.0	36.0
35	34.016125760010084	36.0	36.0	36.0	32.0	36.0
36	33.96786609121171	36.0	36.0	36.0	32.0	36.0
37	33.95672326707201	36.0	36.0	36.0	32.0	36.0
38	33.38209537010785	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	4.0
8	3.0
9	9.0
10	20.0
11	17.0
12	12.0
13	14.0
14	123.0
15	376.0
16	623.0
17	677.0
18	888.0
19	1051.0
20	1553.0
21	2425.0
22	3805.0
23	6012.0
24	9435.0
25	14583.0
26	21300.0
27	31576.0
28	44383.0
29	60112.0
30	81411.0
31	110037.0
32	151328.0
33	228699.0
34	514514.0
35	1095733.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.58178089704294	16.869097405634193	11.352158831307344	26.19696286601552
2	16.690979651481662	18.558057180273742	38.25640805804184	26.494555110202754
3	19.653228970710185	22.265207203104666	27.987773575901375	30.093790250283774
4	12.598523098636512	14.663790713499617	35.62281048112708	37.11487570673679
5	14.766205181625727	36.186447797305014	33.833840182801836	15.213506838267415
6	35.60725899877096	34.970727269214194	16.138771813834	13.283241918180849
7	31.350308288700536	29.713704618302927	19.983215182950726	18.95277191004581
8	28.167882337869088	34.10353612255142	18.496796130574218	19.23178540900528
9	27.090494166253276	14.200889899975511	18.28247540551859	40.426140528252624
10	16.014866134641174	26.41620388513861	31.56856790381705	26.00036207640316
11	38.131884333742036	20.654964953246495	22.157520738848653	19.05562997416281
12	25.27689707258635	23.015514973108388	28.687116389181522	23.02047156512374
13	29.93164070705153	19.27490921556535	24.92862436165225	25.86482571573087
14	23.150356290844904	19.80979618138886	24.528512719392026	32.51133480837421
15	24.967858817439765	28.25109111962234	21.507997062337928	25.273053000599973
16	25.619314634939382	25.636244178631472	24.31216902321994	24.432272163209205
17	24.199545418261415	26.351288331701824	24.990021888824934	24.459144361211827
18	24.914611826687317	24.7144521189835	26.601708225534832	23.76922782879435
19	25.80311542497465	24.621619782385693	24.95561935055126	24.619645442088398
20	26.119853145031886	24.438078115417248	24.842349343437313	24.599719396113553
21	26.55538580255341	24.14656409795416	24.23927248073266	25.058777618759777
22	26.149921404849458	24.784524946714086	24.799227406729692	24.26632624170676
23	24.373631331476865	24.66440972448306	25.619920462321684	25.342038481718394
24	25.248207524301925	24.728455738127774	25.389698311226798	24.633638426343502
25	24.991492661843182	24.68295986468921	25.31359518850161	25.011952284966
26	24.366557070151572	25.79426664302765	25.74906123148616	24.090115055334614
27	25.042154761534697	24.985318296804806	25.161162673271402	24.811364268389095
28	24.50049210427822	24.932527315673028	25.356287278001744	25.21069330204701
29	24.536021236383313	24.86898447053763	25.380821973052914	25.214172320026147
30	24.21969938500067	25.13885896012565	25.785950325393912	24.85549132947977
31	25.539538578315796	24.629137456581308	24.65366909756746	25.177654867535438
32	24.800530630110654	25.105668147827032	24.624942135609768	25.46885908645255
33	24.2612958513266	25.0197855930637	25.531312486347517	25.18760606926218
34	25.2097320722663	24.893527103223047	25.5855064110589	24.311234413451754
35	25.280151643603922	24.78450358313469	25.75529824166351	24.180046531597878
36	24.12035897302325	25.28842445594044	25.52023949459448	25.07097707644183
37	25.265481167944902	24.938993626939677	24.779572600419495	25.015952604695922
38	24.548304113612456	25.2683795236799	25.084139017988864	25.099177344718775
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	21.0
1	53.5
2	86.0
3	86.0
4	243.0
5	400.0
6	400.0
7	512.5
8	625.0
9	621.0
10	617.0
11	617.0
12	931.0
13	1245.0
14	1910.5
15	2576.0
16	2576.0
17	4091.5
18	5607.0
19	5607.0
20	7494.0
21	9381.0
22	11224.0
23	13067.0
24	13067.0
25	17214.5
26	21362.0
27	21362.0
28	28591.5
29	35821.0
30	46618.0
31	57415.0
32	57415.0
33	74317.0
34	91219.0
35	91219.0
36	102332.0
37	113445.0
38	126749.5
39	140054.0
40	140054.0
41	146480.5
42	152907.0
43	167512.0
44	182117.0
45	182117.0
46	187311.5
47	192506.0
48	192506.0
49	203561.0
50	214616.0
51	220228.5
52	225841.0
53	225841.0
54	218234.0
55	210627.0
56	210627.0
57	200643.0
58	190659.0
59	172440.0
60	154221.0
61	154221.0
62	142733.5
63	131246.0
64	110005.5
65	88765.0
66	88765.0
67	73997.0
68	59229.0
69	59229.0
70	46893.0
71	34557.0
72	26712.0
73	18867.0
74	18867.0
75	14445.0
76	10023.0
77	10023.0
78	9039.5
79	8056.0
80	6301.5
81	4547.0
82	4547.0
83	4377.0
84	4207.0
85	4207.0
86	2885.0
87	1563.0
88	1296.5
89	1030.0
90	1030.0
91	760.0
92	490.0
93	419.5
94	349.0
95	349.0
96	328.0
97	307.0
98	307.0
99	680.5
100	1054.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1242058616597885
2	0.05620136723057052
3	0.012895231494607735
4	0.0027302607399006603
5	2.1002005691543542E-4
6	4.620441252139579E-4
7	8.400802276617417E-5
8	4.2004011383087083E-4
9	3.360320910646967E-4
10	5.04048136597045E-4
11	0.00201619254638818
12	0.0023942286488359637
13	0.015163448109294436
14	0.009492906572577682
15	0.025244410841235337
16	0.0113830870848166
17	0.021632065862289846
18	0.005292505434268973
19	0.007686734083104936
20	0.006090581650547627
21	0.005964569616398366
22	0.0071826859465078914
23	0.009156874481512985
24	0.015037436075145176
25	0.017935712860578188
26	0.02011992145249871
27	0.008862846401831376
28	0.005544529502567496
29	0.011845131210030559
30	0.00302428881958227
31	0.005292505434268973
32	0.006678637809910847
33	0.008610822333532853
34	0.012895231494607735
35	0.01709563263291644
36	0.015919520314190007
37	0.009912946686408552
38	0.0059225656050152795
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2380725.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.95086576859263
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.85764728765804	40.167280990500245
2	13.921441976265836	14.743048107810065
3	4.544425185756033	7.21893744019138
4	1.9675465569693968	4.167331745261724
5	1.0485260340490452	2.7760180641902883
6	0.6146549296640346	1.9527906404786421
7	0.40204443796793554	1.490202074749459
8	0.27541729766614015	1.1666867487254644
9	0.2075868161226256	0.9892711472254799
>10	1.0242907225329243	9.458398888378932
>50	0.06671052451283571	2.5024895401927565
>100	0.05631583482935674	6.497169956991655
>500	0.00908769728957866	3.239625374283919
>1k	0.004304698716115779	3.6307492810200763
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3161	0.13277467998193826	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2871	0.12059351668084302	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2814	0.11819928803200705	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2590	0.10879038948219555	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.400802276617417E-5
2	0.0	0.0	0.0	0.0	8.400802276617417E-5
3	0.0	0.0	0.0	8.400802276617417E-5	8.400802276617417E-5
4	0.0	0.0	0.0	8.400802276617417E-5	8.400802276617417E-5
5	0.0	0.0	0.0	8.400802276617417E-5	8.400802276617417E-5
6	0.0	0.0	0.0	8.400802276617417E-5	8.400802276617417E-5
7	0.0	0.0	0.0	8.400802276617417E-5	8.400802276617417E-5
8	0.0	0.0	0.0	8.400802276617417E-5	8.400802276617417E-5
9	0.0	0.0	0.0	8.400802276617417E-5	8.400802276617417E-5
10	0.0	0.0	0.0	1.2601203414926124E-4	8.400802276617417E-5
11	0.0	0.0	0.0	1.2601203414926124E-4	8.400802276617417E-5
12	0.0	0.0	0.0	1.2601203414926124E-4	2.520240682985225E-4
13	0.0	0.0	0.0	1.2601203414926124E-4	3.7803610244778376E-4
14	0.0	0.0	0.0	1.6801604553234834E-4	3.7803610244778376E-4
15	0.0	0.0	0.0	2.520240682985225E-4	5.04048136597045E-4
16	0.0	0.0	0.0	2.520240682985225E-4	5.04048136597045E-4
17	0.0	0.0	0.0	2.940280796816096E-4	5.04048136597045E-4
18	0.0	0.0	0.0	4.2004011383087083E-4	5.46052147980132E-4
19	0.0	0.0	0.0	4.6204412521395796E-4	5.46052147980132E-4
20	0.0	0.0	0.0	5.880561593632192E-4	5.880561593632192E-4
21	0.0	0.0	4.2004011383087086E-5	6.720641821293934E-4	6.300601707463063E-4
22	0.0	0.0	4.2004011383087086E-5	7.560722048955675E-4	6.300601707463063E-4
23	0.0	0.0	4.2004011383087086E-5	9.66092261811003E-4	6.300601707463063E-4
24	0.0	0.0	4.2004011383087086E-5	0.0011761123187264385	6.300601707463063E-4
25	0.0	0.0	4.2004011383087086E-5	0.0013861323756418737	6.300601707463063E-4
26	0.0	0.0	4.2004011383087086E-5	0.0018061724894727447	7.140681935124804E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3015	0.0	19.917418	1
GGTATCA	1115	0.0	19.101427	1
CTAGCGG	525	0.0	17.980742	29
CGTTCGG	45	0.008850369	17.778688	14
GTAGGAC	1745	0.0	17.051731	3
TGTAGGA	1765	0.0	16.955202	2
TCTAGCG	560	0.0	16.856236	28
TAGCGGC	570	0.0	16.841553	30
GTCCTAA	900	0.0	16.54739	1
TAGGACG	1475	0.0	16.267576	4
TATACTG	295	0.0	16.267576	5
AACCGCG	240	0.0	15.997795	7
TACCGTC	465	0.0	15.825774	7
ATACCGT	480	0.0	15.663193	6
ACCGTCG	440	0.0	15.634537	8
GTCCTAC	1530	0.0	15.490283	1
CTGTAGG	1815	0.0	15.440113	1
CGCGGTC	250	0.0	15.360786	10
GTGTATA	535	0.0	15.265305	1
TTAGGAC	920	0.0	15.128031	3
>>END_MODULE