FastQCFastQC Report
Thu 2 Feb 2017
SRR4062952_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062952_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2380725
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT31970.1342868243917294No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT29300.12307175335244515No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC27880.1171071837360468No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT27160.11408289491646453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG5200.019.07644729
TCTAGCG5300.019.01839428
GGTATCA11250.018.917071
GTATCAA27250.018.7317751
TAAACCG601.1381339E-418.6681615
TAGCGGC5650.017.55708530
TGTAGGA17100.016.7484722
GTCCTAC15950.016.3524071
ACGTTTT7150.016.33525729
TTATCGA703.6974045E-416.0006084
CGTTTTT7250.015.88925930
GTAGGAC17500.015.8177443
TTAGGAC8900.015.6410453
TAGGACG15350.015.4272984
CACGTTT7700.015.3762428
TCACGTT7300.015.34208327
TCCTACA16950.015.2919752
GTCTAAC951.4325888E-515.1591091
CTGTAGG18900.014.9006631
GTCCTAA9050.014.8520171
Produced by FastQC (version 0.11.5)