##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062952_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2380725
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.278612187463903	32.0	32.0	32.0	32.0	32.0
2	31.335224773965912	32.0	32.0	32.0	32.0	32.0
3	31.41160402818469	32.0	32.0	32.0	32.0	32.0
4	31.50216803704754	32.0	32.0	32.0	32.0	32.0
5	31.427176175324743	32.0	32.0	32.0	32.0	32.0
6	34.98146026945573	36.0	36.0	36.0	36.0	36.0
7	35.0134417036827	36.0	36.0	36.0	36.0	36.0
8	34.93860819708282	36.0	36.0	36.0	36.0	36.0
9	35.04441462159636	36.0	36.0	36.0	36.0	36.0
10	34.90923941236388	36.0	36.0	36.0	32.0	36.0
11	35.07731972403364	36.0	36.0	36.0	36.0	36.0
12	34.98974262042025	36.0	36.0	36.0	36.0	36.0
13	35.036590114355924	36.0	36.0	36.0	36.0	36.0
14	34.9772044230224	36.0	36.0	36.0	36.0	36.0
15	34.950915372418066	36.0	36.0	36.0	36.0	36.0
16	34.96736708355648	36.0	36.0	36.0	36.0	36.0
17	34.93495888857386	36.0	36.0	36.0	32.0	36.0
18	34.94039966816831	36.0	36.0	36.0	36.0	36.0
19	34.93047328019826	36.0	36.0	36.0	36.0	36.0
20	34.92543741927354	36.0	36.0	36.0	32.0	36.0
21	34.91669218410358	36.0	36.0	36.0	32.0	36.0
22	34.89822638061935	36.0	36.0	36.0	32.0	36.0
23	34.848111814678305	36.0	36.0	36.0	32.0	36.0
24	34.831373636182256	36.0	36.0	36.0	32.0	36.0
25	34.80835501790421	36.0	36.0	36.0	32.0	36.0
26	34.74568587300087	36.0	36.0	36.0	32.0	36.0
27	34.733997836793414	36.0	36.0	36.0	32.0	36.0
28	34.70137920171376	36.0	36.0	36.0	32.0	36.0
29	34.672403154501254	36.0	36.0	36.0	32.0	36.0
30	34.65087903894822	36.0	36.0	36.0	32.0	36.0
31	34.643816064434155	36.0	36.0	36.0	32.0	36.0
32	34.62465929496267	36.0	36.0	36.0	32.0	36.0
33	34.60509466654065	36.0	36.0	36.0	32.0	36.0
34	34.59950729294648	36.0	36.0	36.0	32.0	36.0
35	34.58246626552836	36.0	36.0	36.0	32.0	36.0
36	34.54739543626416	36.0	36.0	36.0	32.0	36.0
37	34.538112969788614	36.0	36.0	36.0	32.0	36.0
38	34.12945804324313	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	6.0
21	30.0
22	130.0
23	450.0
24	1234.0
25	3224.0
26	6883.0
27	13864.0
28	24428.0
29	39332.0
30	60002.0
31	87966.0
32	128550.0
33	210424.0
34	533355.0
35	1270847.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.08025981161893	18.016386514731817	11.927146214189156	26.976207459460095
2	15.731510359239307	19.459912421636265	37.510926293461026	27.2976509256634
3	18.613195560175996	22.985561121087063	28.36207457812221	30.03916874061473
4	12.18688608460539	15.526585831278986	35.503290386995886	36.783237697119745
5	14.229494893150152	36.70438068419523	33.55227233396227	15.513852088692348
6	35.706244922750194	35.314094319444806	15.816488975174718	13.16317178263028
7	31.191566899677998	29.90333992239303	19.942462639359455	18.96263053856952
8	28.060229875594317	34.38922257053555	18.266637375303432	19.283910178566703
9	26.9563566390004	14.080265071911423	18.12670306308774	40.83667522600044
10	15.787793506111647	26.734951904901923	31.53131431931785	25.945940269668583
11	38.24875210607697	20.614287676567066	22.059084831583153	19.077875385772813
12	25.078978108751	23.15061433018457	28.645499707441815	23.124907853622616
13	29.909372030837705	19.27339689388345	24.898287158265433	25.918943917013408
14	23.042990283628008	19.798893109827095	24.49943798609163	32.65867862045327
15	24.929380755862184	28.449064885696586	21.343162271996977	25.278392086444256
16	25.639217313724732	25.78786957244939	24.048566738521558	24.52434637530432
17	24.115721051360406	26.508815591889025	24.854445599554758	24.52101775719581
18	24.88221025107898	24.83676191076248	26.46576148022136	23.815266357937183
19	25.788740824748764	24.66181520335192	24.915435424083	24.634008547816315
20	26.072309905595986	24.456583603734156	24.747461382562037	24.723645108107824
21	26.53741175996189	24.203497930459754	24.23075856819906	25.028331741379294
22	26.097061230113194	24.876886190923432	24.677619077221888	24.348433501741486
23	24.37894890709625	24.7312789985559	25.47357482309988	25.416197271247963
24	25.258356173014523	24.756072204895567	25.290657257768117	24.694914364321793
25	24.973537461713327	24.7411291691099	25.28512334903164	25.00021002014513
26	24.34300475695429	25.854729126631593	25.64567516197797	24.15659095443615
27	25.0833886737578	25.090151336634015	24.927343378197932	24.89911661141025
28	24.42806288000504	25.0378771172647	25.34921084963614	25.18484915309412
29	24.57553896397106	24.828781148599692	25.284692688151715	25.31098719927753
30	24.238288756576253	25.09601066901889	25.746820821388443	24.918879753016412
31	25.48034737317414	24.66017704690798	24.6139306303752	25.24554494954268
32	24.831637421373742	25.199424545044053	24.535383129088828	25.433554904493377
33	24.23530647176805	24.998645370632897	25.531760283107037	25.23428787449201
34	25.1464154826786	24.951390857826922	25.555072509424647	24.34712115006983
35	25.25638198447952	24.86645874681032	25.651723739617132	24.22543552909303
36	24.119039368259667	25.327578783773852	25.402009891944683	25.1513719560218
37	25.217066229824947	24.96273194090036	24.681599092713356	25.138602736561346
38	24.585682584660205	25.142824259689178	25.05541383856921	25.21607931708141
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	21.0
2	34.0
3	34.0
4	153.5
5	273.0
6	273.0
7	373.0
8	473.0
9	539.5
10	606.0
11	606.0
12	888.5
13	1171.0
14	1845.0
15	2519.0
16	2519.0
17	4046.0
18	5573.0
19	5573.0
20	7377.5
21	9182.0
22	11124.0
23	13066.0
24	13066.0
25	17149.5
26	21233.0
27	21233.0
28	28245.5
29	35258.0
30	46187.0
31	57116.0
32	57116.0
33	74245.5
34	91375.0
35	91375.0
36	102694.5
37	114014.0
38	128241.5
39	142469.0
40	142469.0
41	148244.0
42	154019.0
43	168896.0
44	183773.0
45	183773.0
46	187866.0
47	191959.0
48	191959.0
49	203494.0
50	215029.0
51	220017.0
52	225005.0
53	225005.0
54	218240.0
55	211475.0
56	211475.0
57	201478.0
58	191481.0
59	172922.5
60	154364.0
61	154364.0
62	143154.5
63	131945.0
64	110242.5
65	88540.0
66	88540.0
67	73671.0
68	58802.0
69	58802.0
70	46495.5
71	34189.0
72	26304.5
73	18420.0
74	18420.0
75	13938.5
76	9457.0
77	9457.0
78	8750.5
79	8044.0
80	6166.0
81	4288.0
82	4288.0
83	3944.5
84	3601.0
85	3601.0
86	2344.0
87	1087.0
88	865.5
89	644.0
90	644.0
91	397.5
92	151.0
93	99.0
94	47.0
95	47.0
96	30.0
97	13.0
98	13.0
99	17.5
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009702926629493116
2	0.0
3	0.0
4	8.400802276617417E-5
5	4.2004011383087086E-5
6	2.9402807968160956E-4
7	4.620441252139579E-4
8	0.001932184523622006
9	0.0036963530017116632
10	0.0010501002845771771
11	0.004788457297671927
12	8.400802276617417E-5
13	1.2601203414926124E-4
14	4.2004011383087086E-5
15	0.0
16	4.2004011383087086E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	1.2601203414926124E-4
22	4.2004011383087086E-5
23	1.2601203414926124E-4
24	0.0
25	4.2004011383087086E-5
26	0.0
27	2.520240682985225E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	8.400802276617417E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2380725.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.0086761095233
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.06783737390342	37.781023267382835
2	14.942001646425283	15.24343444820942
3	4.888790507713249	7.481121947257714
4	2.116665321421612	4.3187318325062005
5	1.1190782465210583	2.854134990900296
6	0.6514383645231338	1.9937405124766858
7	0.4383858908456644	1.5653038741991931
8	0.295875773629314	1.207378520456985
9	0.22788391717237436	1.046165122945357
>10	1.105720845398703	9.891825566764446
>50	0.0707423426168316	2.539784705536099
>100	0.06075223545653379	6.68637717175585
>500	0.009940246506987728	3.420441589542681
>1k	0.0048872878659353665	3.9705364500662395
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3197	0.1342868243917294	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2930	0.12307175335244515	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2788	0.1171071837360468	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2716	0.11408289491646453	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.400802276617417E-5
2	0.0	0.0	0.0	0.0	8.400802276617417E-5
3	0.0	0.0	0.0	0.0	8.400802276617417E-5
4	0.0	0.0	0.0	0.0	8.400802276617417E-5
5	0.0	0.0	0.0	0.0	8.400802276617417E-5
6	0.0	0.0	0.0	0.0	8.400802276617417E-5
7	0.0	0.0	0.0	0.0	8.400802276617417E-5
8	0.0	0.0	0.0	0.0	8.400802276617417E-5
9	0.0	0.0	0.0	4.2004011383087086E-5	8.400802276617417E-5
10	0.0	0.0	0.0	4.2004011383087086E-5	8.400802276617417E-5
11	0.0	0.0	0.0	4.2004011383087086E-5	1.2601203414926124E-4
12	0.0	0.0	0.0	4.2004011383087086E-5	2.1002005691543542E-4
13	0.0	0.0	0.0	4.2004011383087086E-5	2.1002005691543542E-4
14	0.0	0.0	0.0	4.2004011383087086E-5	2.1002005691543542E-4
15	0.0	0.0	0.0	1.2601203414926124E-4	4.2004011383087083E-4
16	0.0	0.0	0.0	1.2601203414926124E-4	4.2004011383087083E-4
17	0.0	0.0	0.0	1.2601203414926124E-4	4.2004011383087083E-4
18	0.0	0.0	0.0	1.6801604553234834E-4	4.2004011383087083E-4
19	0.0	0.0	0.0	1.6801604553234834E-4	4.2004011383087083E-4
20	0.0	0.0	0.0	2.520240682985225E-4	4.2004011383087083E-4
21	0.0	0.0	0.0	2.940280796816096E-4	4.2004011383087083E-4
22	0.0	0.0	0.0	3.360320910646967E-4	4.2004011383087083E-4
23	0.0	0.0	0.0	5.880561593632192E-4	4.2004011383087083E-4
24	0.0	0.0	0.0	7.560722048955675E-4	4.2004011383087083E-4
25	0.0	0.0	0.0	8.820842390448287E-4	4.2004011383087083E-4
26	0.0	0.0	0.0	0.001092104295960264	4.2004011383087083E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	520	0.0	19.076447	29
TCTAGCG	530	0.0	19.018394	28
GGTATCA	1125	0.0	18.91707	1
GTATCAA	2725	0.0	18.731775	1
TAAACCG	60	1.1381339E-4	18.668161	5
TAGCGGC	565	0.0	17.557085	30
TGTAGGA	1710	0.0	16.748472	2
GTCCTAC	1595	0.0	16.352407	1
ACGTTTT	715	0.0	16.335257	29
TTATCGA	70	3.6974045E-4	16.000608	4
CGTTTTT	725	0.0	15.889259	30
GTAGGAC	1750	0.0	15.817744	3
TTAGGAC	890	0.0	15.641045	3
TAGGACG	1535	0.0	15.427298	4
CACGTTT	770	0.0	15.37624	28
TCACGTT	730	0.0	15.342083	27
TCCTACA	1695	0.0	15.291975	2
GTCTAAC	95	1.4325888E-5	15.159109	1
CTGTAGG	1890	0.0	14.900663	1
GTCCTAA	905	0.0	14.852017	1
>>END_MODULE