FastQCFastQC Report
Thu 2 Feb 2017
SRR4062951_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062951_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73813
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2670.3617248994079634No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT1710.23166650860959453No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT900.1219297413734708No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGCTA200.00373098432.05
TGATTTC350.002048386522.85714317
GGTATCA350.002048386522.8571431
CTGTGCT400.004444528420.04
AGACTGT600.00241296916.06
GTATCAA650.00411273514.7692311
CCTGGAC650.00411273514.7692313
GTACATG1455.8836762E-511.0344831
TACATGG1601.5962198E-410.02
ACATGGG1457.238574E-49.9310343
Produced by FastQC (version 0.11.5)