##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062951_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	73813
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.360844295720266	32.0	32.0	32.0	32.0	32.0
2	31.068741278636555	32.0	32.0	32.0	32.0	32.0
3	31.092707246690964	32.0	32.0	32.0	32.0	32.0
4	31.15559589774159	32.0	32.0	32.0	32.0	32.0
5	31.128757806890384	32.0	32.0	32.0	32.0	32.0
6	34.770568869982256	36.0	36.0	36.0	32.0	36.0
7	34.75586956227223	36.0	36.0	36.0	32.0	36.0
8	34.76230474306694	36.0	36.0	36.0	32.0	36.0
9	34.8233102570008	36.0	36.0	36.0	32.0	36.0
10	34.6524324983404	36.0	36.0	36.0	32.0	36.0
11	34.82140002438595	36.0	36.0	36.0	32.0	36.0
12	34.73398994757021	36.0	36.0	36.0	32.0	36.0
13	34.74673837941826	36.0	36.0	36.0	32.0	36.0
14	34.678389985503905	36.0	36.0	36.0	32.0	36.0
15	34.659612805332394	36.0	36.0	36.0	32.0	36.0
16	34.65103708018913	36.0	36.0	36.0	32.0	36.0
17	34.619768875401356	36.0	36.0	36.0	32.0	36.0
18	34.60191294216466	36.0	36.0	36.0	32.0	36.0
19	34.628141384309	36.0	36.0	36.0	32.0	36.0
20	34.6178586427865	36.0	36.0	36.0	32.0	36.0
21	34.57984365897606	36.0	36.0	36.0	32.0	36.0
22	34.579586251744274	36.0	36.0	36.0	32.0	36.0
23	34.54610976386273	36.0	36.0	36.0	32.0	36.0
24	34.491241380244674	36.0	36.0	36.0	32.0	36.0
25	34.48942598187311	36.0	36.0	36.0	32.0	36.0
26	34.462601438770946	36.0	36.0	36.0	32.0	36.0
27	34.4911194505033	36.0	36.0	36.0	32.0	36.0
28	34.47857423489087	36.0	36.0	36.0	32.0	36.0
29	34.43240350615745	36.0	36.0	36.0	32.0	36.0
30	34.391990570766666	36.0	36.0	36.0	32.0	36.0
31	34.445924159700866	36.0	36.0	36.0	32.0	36.0
32	34.419749908552696	36.0	36.0	36.0	32.0	36.0
33	34.36591115386179	36.0	36.0	36.0	32.0	36.0
34	34.36270033733895	36.0	36.0	36.0	32.0	36.0
35	34.35552003034696	36.0	36.0	36.0	32.0	36.0
36	34.32081069730264	36.0	36.0	36.0	32.0	36.0
37	34.33973690271361	36.0	36.0	36.0	32.0	36.0
38	33.80806903932911	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	18.0
16	19.0
17	25.0
18	31.0
19	35.0
20	61.0
21	54.0
22	101.0
23	161.0
24	199.0
25	313.0
26	517.0
27	794.0
28	1137.0
29	1544.0
30	2012.0
31	2864.0
32	3887.0
33	6085.0
34	14584.0
35	39369.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.90598800770545	17.64793661990938	12.195783704588003	25.250291667797164
2	15.957432386633227	20.27384260828306	39.07001965701891	24.698705348064802
3	18.0922701713976	24.660930831244496	29.741887406002306	27.5049115913556
4	13.346430023032111	16.299959355100935	36.08454139005555	34.26906923181141
5	14.710145909257177	36.432606722393075	34.510181133404686	14.347066234945064
6	33.44984555356852	35.30997669755595	17.84127242182843	13.398905327047093
7	28.92715375340387	31.60825328871608	21.855228753742566	17.60936420413748
8	27.3515505398778	34.07665316407679	20.12247165133513	18.449324644710284
9	27.01285681384038	15.601587796187664	18.70537710159457	38.680178288377384
10	16.41332032731805	27.1094131035604	32.56787514225329	23.90939142686826
11	35.93318159648838	21.676692136779927	23.43656857963475	18.95355768709695
12	24.074350046739735	24.059447522794088	29.914784658528987	21.951417771937194
13	29.740386439391887	20.069916804422643	25.296062437332324	24.89363431885315
14	23.32235906190302	19.379750436938583	26.652576244089477	30.64531425706892
15	24.367869047941678	27.388276105043495	23.052115233475163	25.19173961353966
16	24.604045577097644	26.8070289531087	24.326301670527982	24.262623799265672
17	24.004065040650406	26.527100271002713	25.60569105691057	23.863143631436316
18	24.15764587934048	26.78327078619718	25.748194713525084	23.31088862093726
19	24.46856074462464	26.315219011231694	26.294896286360743	22.921323957782928
20	26.239991329205676	25.368847461760442	25.43116879597892	22.959992413054962
21	25.42949273791459	25.685562540646	25.45659007153696	23.42835464990245
22	25.783112941686536	24.917353132451765	25.333297203555173	23.966236722306526
23	24.90718911741593	25.602254559249925	25.31095032924153	24.179605994092622
24	24.1680667714487	26.21131645981248	25.30892634545553	24.31169042328329
25	24.524416020811877	25.34957454880494	26.16795837623977	23.958051054143407
26	24.1378843105107	26.33704150350266	25.62973401444425	23.89534017154239
27	24.714457978240546	25.722492446515915	25.09518067391982	24.467868901323722
28	24.49466211456132	25.929388175364437	25.677396629274373	23.89855308079987
29	25.030485326396228	25.357017044684717	25.988402026935482	23.624095601983576
30	24.475695008941635	25.485015986560448	25.40643797756462	24.632851026933288
31	25.312631250931457	25.328889430828216	25.64321424216559	23.715265076074736
32	24.2904174174581	25.9676741949032	25.875547005188928	23.86636138244977
33	24.836742988754914	25.779704647066794	25.882671724698554	23.500880639479746
34	25.272685392192727	25.48947874747639	25.775374984756716	23.462460875574166
35	24.953591414750477	25.524044389642413	25.28692023143318	24.23544396417393
36	24.202967278639658	25.24896687216313	26.19470225594472	24.35336359325249
37	24.38148660000542	25.358371948080098	25.90304311302604	24.357098338888434
38	24.138304790808583	25.69369174073271	25.975504010405377	24.192499458053327
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	3.5
2	4.0
3	4.0
4	13.5
5	23.0
6	23.0
7	30.5
8	38.0
9	32.5
10	27.0
11	27.0
12	42.0
13	57.0
14	84.5
15	112.0
16	112.0
17	187.0
18	262.0
19	262.0
20	372.0
21	482.0
22	523.5
23	565.0
24	565.0
25	705.5
26	846.0
27	846.0
28	1038.0
29	1230.0
30	1598.0
31	1966.0
32	1966.0
33	2419.5
34	2873.0
35	2873.0
36	3327.0
37	3781.0
38	4418.0
39	5055.0
40	5055.0
41	5394.0
42	5733.0
43	6053.5
44	6374.0
45	6374.0
46	6666.5
47	6959.0
48	6959.0
49	7130.5
50	7302.0
51	6941.5
52	6581.0
53	6581.0
54	6316.5
55	6052.0
56	6052.0
57	5570.5
58	5089.0
59	4463.0
60	3837.0
61	3837.0
62	3321.0
63	2805.0
64	2455.5
65	2106.0
66	2106.0
67	1721.5
68	1337.0
69	1337.0
70	1128.0
71	919.0
72	717.0
73	515.0
74	515.0
75	405.5
76	296.0
77	296.0
78	242.5
79	189.0
80	125.5
81	62.0
82	62.0
83	46.0
84	30.0
85	30.0
86	18.0
87	6.0
88	6.0
89	6.0
90	6.0
91	4.5
92	3.0
93	4.5
94	6.0
95	6.0
96	4.5
97	3.0
98	3.0
99	141.0
100	279.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1341227155108179
2	0.06502919539918443
3	0.010838199233197404
4	0.004064324712449027
5	0.0
6	0.0013547749041496755
7	0.0
8	0.0
9	0.0
10	0.0013547749041496755
11	0.0013547749041496755
12	0.0
13	0.014902523945646432
14	0.005419099616598702
15	0.020321623562245133
16	0.005419099616598702
17	0.017612073753945783
18	0.002709549808299351
19	0.005419099616598702
20	0.002709549808299351
21	0.0067738745207483785
22	0.0067738745207483785
23	0.009483424329047729
24	0.01219297413734708
25	0.01219297413734708
26	0.01625729884979611
27	0.008128649424898054
28	0.0013547749041496755
29	0.009483424329047729
30	0.0013547749041496755
31	0.005419099616598702
32	0.002709549808299351
33	0.004064324712449027
34	0.013547749041496757
35	0.01625729884979611
36	0.010838199233197404
37	0.009483424329047729
38	0.0067738745207483785
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	73813.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.64372129570672
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.060817428986965	27.870429328167123
2	16.164554332017453	14.756208255998265
3	8.016977827906564	10.977741048324821
4	4.698584191623876	8.578434693075746
5	2.8523938143717906	6.509693414439191
6	1.840254073788252	5.039762643436793
7	1.335668279362441	4.267540948071478
8	0.958713009409041	3.5007383523227618
9	0.7746876020302158	3.182366249847588
>10	2.285476833575732	14.525896522292822
>50	0.005936303463833071	0.1977971360058526
>100	0.005936303463833071	0.5933914080175579
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	267	0.3617248994079634	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	171	0.23166650860959453	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	90	0.1219297413734708	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0013547749041496755	0.0
21	0.0	0.0	0.0	0.0013547749041496755	0.0
22	0.0	0.0	0.0	0.0013547749041496755	0.0
23	0.0	0.0	0.0	0.0013547749041496755	0.0
24	0.0	0.0	0.0	0.0013547749041496755	0.0
25	0.0	0.0	0.0	0.0013547749041496755	0.0
26	0.0	0.0	0.0	0.0013547749041496755	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGAGCTA	20	0.003730984	32.0	5
TGATTTC	35	0.0020483865	22.857143	17
GGTATCA	35	0.0020483865	22.857143	1
CTGTGCT	40	0.0044445284	20.0	4
AGACTGT	60	0.002412969	16.0	6
GTATCAA	65	0.004112735	14.769231	1
CCTGGAC	65	0.004112735	14.769231	3
GTACATG	145	5.8836762E-5	11.034483	1
TACATGG	160	1.5962198E-4	10.0	2
ACATGGG	145	7.238574E-4	9.931034	3
>>END_MODULE