FastQCFastQC Report
Thu 2 Feb 2017
SRR4062951_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062951_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73813
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT1400.1896684865809546No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGAT930.12599406608591984No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT820.11109154214027339No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGTGC200.00373098432.08
GGTATCA252.869056E-432.01
TTGTGCA308.342103E-426.6666669
GTATCAA1151.8189894E-1220.8695661
TTTCCCA400.004444528420.024
TATTACA507.109032E-419.22
TGGACCT550.001348320317.4545464
TATCAAC1304.5602064E-916.02
GAGTACT650.00411273514.76923112
CAACGCA1451.9292202E-814.3448285
TACTTTT901.5036872E-414.22222215
ATCAACG1503.012974E-813.8666683
AACGCAG1503.012974E-813.8666686
TCAACGC1503.012974E-813.8666684
GTACTTT700.00672469613.71428614
CTTTTTT850.001583607913.17647117
ACTTTTT850.001583607913.17647116
ACGCAGA1701.5487967E-712.2352947
AGAGTAC1701.5487967E-712.23529411
CGCAGAG1906.550672E-710.9473698
Produced by FastQC (version 0.11.5)