##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062951_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	73813
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.295774457073957	32.0	32.0	32.0	32.0	32.0
2	31.43111646999851	32.0	32.0	32.0	32.0	32.0
3	31.4511264953328	32.0	32.0	32.0	32.0	32.0
4	31.525124300597454	32.0	32.0	32.0	32.0	32.0
5	31.499193908932032	32.0	32.0	32.0	32.0	32.0
6	35.02832834324577	36.0	36.0	36.0	36.0	36.0
7	35.01749014401257	36.0	36.0	36.0	36.0	36.0
8	34.98547681302752	36.0	36.0	36.0	36.0	36.0
9	35.08123230325281	36.0	36.0	36.0	36.0	36.0
10	34.949101106851096	36.0	36.0	36.0	36.0	36.0
11	35.083806375570695	36.0	36.0	36.0	36.0	36.0
12	34.993537723707206	36.0	36.0	36.0	36.0	36.0
13	35.03857044152114	36.0	36.0	36.0	36.0	36.0
14	35.01628439434788	36.0	36.0	36.0	36.0	36.0
15	34.99302290924363	36.0	36.0	36.0	36.0	36.0
16	35.01471285545907	36.0	36.0	36.0	36.0	36.0
17	35.00173411187731	36.0	36.0	36.0	36.0	36.0
18	34.94285559454297	36.0	36.0	36.0	36.0	36.0
19	34.984636852586945	36.0	36.0	36.0	36.0	36.0
20	34.95800197797136	36.0	36.0	36.0	36.0	36.0
21	34.94533483261756	36.0	36.0	36.0	36.0	36.0
22	34.96650996436942	36.0	36.0	36.0	36.0	36.0
23	34.90993456437213	36.0	36.0	36.0	32.0	36.0
24	34.87289501849268	36.0	36.0	36.0	32.0	36.0
25	34.87958760651917	36.0	36.0	36.0	32.0	36.0
26	34.80468210206874	36.0	36.0	36.0	32.0	36.0
27	34.81780987088995	36.0	36.0	36.0	32.0	36.0
28	34.78110901873654	36.0	36.0	36.0	32.0	36.0
29	34.794778697519405	36.0	36.0	36.0	32.0	36.0
30	34.76410659368946	36.0	36.0	36.0	32.0	36.0
31	34.763442753986425	36.0	36.0	36.0	32.0	36.0
32	34.71476569168033	36.0	36.0	36.0	32.0	36.0
33	34.737553005568124	36.0	36.0	36.0	32.0	36.0
34	34.692384810263775	36.0	36.0	36.0	32.0	36.0
35	34.665519623914484	36.0	36.0	36.0	32.0	36.0
36	34.644805115630035	36.0	36.0	36.0	32.0	36.0
37	34.60070719249997	36.0	36.0	36.0	32.0	36.0
38	34.29479901914297	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	0.0
22	8.0
23	12.0
24	37.0
25	99.0
26	190.0
27	454.0
28	758.0
29	1234.0
30	1838.0
31	2615.0
32	3709.0
33	5830.0
34	14514.0
35	42514.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.115246521332665	18.872193694365034	12.620754129012154	26.391805655290145
2	14.977036565374663	21.141262379255686	38.26426239280343	25.617438662566215
3	16.99836072236598	25.38848170376492	30.315798030157286	27.297359543711814
4	12.677983553032663	17.46304851448932	35.77960521859293	34.07936271388509
5	14.02462980775744	37.40533510357254	34.259547776137	14.310487312533024
6	33.39384661238535	35.64277295327381	17.786839716581092	13.176540717759744
7	28.691811629545334	32.01647428602396	21.520890912046823	17.770823172383896
8	26.881807836124207	34.82902509077115	19.529344821980168	18.75982225112448
9	26.73079528519171	15.28519170844059	18.672266630537866	39.311746375829834
10	15.6598431170661	27.392193787002288	33.06328153577283	23.88468156015878
11	35.82722505995367	21.93152411017925	23.283699378107766	18.95755145175932
12	23.22626095674204	24.410334222968856	29.925622857762185	22.437781962526927
13	29.668215625973744	19.96125343774132	25.667565334019756	24.702965602265184
14	22.86453605733407	19.75532765231057	26.2934713397369	31.086664950618452
15	24.395431699023206	27.840624280275833	22.699253519027813	25.064690501673148
16	24.4360749461477	26.706677685502555	24.37240052565266	24.484846842697085
17	23.46470133987238	26.785254629943232	25.93852031484969	23.811523715334697
18	24.064866622410687	26.59287659355398	25.654017584978256	23.688239199057076
19	24.33853115304892	26.563071545662687	26.212184845487922	22.886212455800468
20	25.606600463333017	25.293647460474446	25.64046983593676	23.45928224025578
21	24.88450543942124	25.612019562949616	25.519894869467436	23.983580128161705
22	25.118881497839133	25.255713763158248	25.735304079227234	23.890100659775378
23	24.8452169672009	25.491444596480296	25.24216601411676	24.42117242220205
24	24.112283744055926	26.415401081110375	24.991532656849063	24.480782517984636
25	24.324983404007426	25.337000257407233	25.89110319320445	24.446913145380893
26	24.03777112432769	26.247408992995812	25.56866676601683	24.146153116659665
27	24.33853115304892	26.193217996829826	25.051142752631648	24.4171080974896
28	24.329047728719875	25.911424816766694	25.685177407773697	24.074350046739735
29	24.47129909365559	25.685177407773697	25.8789102190671	23.96461327950361
30	24.028287699998646	25.94258463956214	25.255713763158248	24.773413897280967
31	24.52142576510913	25.716337230569142	25.820654898188668	23.941582106133065
32	24.284340156882934	25.92632734071234	25.575440640537572	24.21389186186715
33	24.534973514150625	26.30566431387425	25.862652920217304	23.29670925175782
34	25.13649357159308	25.824719222901116	25.675693983444653	23.363093222061153
35	24.34395025266552	26.076707355072955	25.39796512809397	24.18137726416756
36	24.92243913673743	25.330226382886483	25.58085974015417	24.166474740221915
37	24.127186268001573	25.467058648205597	25.847750396271657	24.55800468752117
38	23.948355980653815	25.72175633018574	26.126834026526492	24.203053662633952
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	4.5
2	7.0
3	7.0
4	13.5
5	20.0
6	20.0
7	23.0
8	26.0
9	23.0
10	20.0
11	20.0
12	31.5
13	43.0
14	78.5
15	114.0
16	114.0
17	181.0
18	248.0
19	248.0
20	344.0
21	440.0
22	500.0
23	560.0
24	560.0
25	695.0
26	830.0
27	830.0
28	1047.0
29	1264.0
30	1640.0
31	2016.0
32	2016.0
33	2490.0
34	2964.0
35	2964.0
36	3401.0
37	3838.0
38	4528.0
39	5218.0
40	5218.0
41	5442.0
42	5666.0
43	6035.5
44	6405.0
45	6405.0
46	6679.5
47	6954.0
48	6954.0
49	7176.0
50	7398.0
51	7002.5
52	6607.0
53	6607.0
54	6358.5
55	6110.0
56	6110.0
57	5548.0
58	4986.0
59	4372.0
60	3758.0
61	3758.0
62	3339.0
63	2920.0
64	2508.5
65	2097.0
66	2097.0
67	1697.0
68	1297.0
69	1297.0
70	1097.5
71	898.0
72	717.5
73	537.0
74	537.0
75	417.5
76	298.0
77	298.0
78	241.5
79	185.0
80	123.5
81	62.0
82	62.0
83	37.5
84	13.0
85	13.0
86	11.5
87	10.0
88	5.5
89	1.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008128649424898054
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0013547749041496755
8	0.0013547749041496755
9	0.004064324712449027
10	0.0
11	0.008128649424898054
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	73813.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.0423773590018
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.50995109046726	25.76917345183098
2	17.235227167861208	15.181607575901266
3	8.354609492755852	11.038705919011555
4	4.9740071980067055	8.762684080040101
5	3.10067981174444	6.828065516914365
6	2.0240548771109537	5.348651321582919
7	1.4580577686179212	4.495143131968623
8	1.0366360084899566	3.6524731415875253
9	0.7474853117598204	2.9628927153753404
>10	2.550063059460457	15.53384905098018
>50	0.00615214248361992	0.23708560822619323
>100	0.00307607124180996	0.1896684865809546
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	140	0.1896684865809546	No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGAT	93	0.12599406608591984	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	82	0.11109154214027339	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0013547749041496755	0.0
21	0.0	0.0	0.0	0.0013547749041496755	0.0
22	0.0	0.0	0.0	0.0013547749041496755	0.0
23	0.0	0.0	0.0	0.0013547749041496755	0.0
24	0.0	0.0	0.0	0.0013547749041496755	0.0
25	0.0	0.0	0.0	0.0013547749041496755	0.0
26	0.0	0.0	0.0	0.0013547749041496755	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTGTGC	20	0.003730984	32.0	8
GGTATCA	25	2.869056E-4	32.0	1
TTGTGCA	30	8.342103E-4	26.666666	9
GTATCAA	115	1.8189894E-12	20.869566	1
TTTCCCA	40	0.0044445284	20.0	24
TATTACA	50	7.109032E-4	19.2	2
TGGACCT	55	0.0013483203	17.454546	4
TATCAAC	130	4.5602064E-9	16.0	2
GAGTACT	65	0.004112735	14.769231	12
CAACGCA	145	1.9292202E-8	14.344828	5
TACTTTT	90	1.5036872E-4	14.222222	15
ATCAACG	150	3.012974E-8	13.866668	3
AACGCAG	150	3.012974E-8	13.866668	6
TCAACGC	150	3.012974E-8	13.866668	4
GTACTTT	70	0.006724696	13.714286	14
CTTTTTT	85	0.0015836079	13.176471	17
ACTTTTT	85	0.0015836079	13.176471	16
ACGCAGA	170	1.5487967E-7	12.235294	7
AGAGTAC	170	1.5487967E-7	12.235294	11
CGCAGAG	190	6.550672E-7	10.947369	8
>>END_MODULE