FastQCFastQC Report
Thu 2 Feb 2017
SRR4062950_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062950_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences800559
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT24120.30128947398005645No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA19770.24695244198116567No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT17780.2220948112506386No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC16720.2088540632233227No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG15720.19636279149943978No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT15360.19186593367884192No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC15270.19074171922369246No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA15190.1897424174857818No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG14580.18212274173421322No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC14260.17812553478257068No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC13710.17125533533443507No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG13440.16788269196898667No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG13180.1646349613207771No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA13100.16363565958286647No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG12300.15364264220376012No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT12080.15089456242450586No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG11850.1480215699280128No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT11210.1400271560247277No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA11140.1391527670040559No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA11110.13877802885233942No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT10520.1314081785352485No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC10240.12791062245256127No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT9810.12253937561129162No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT9760.12191481202509748No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT9730.12154007387338098No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC9610.12004112126651502No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC9570.11954147039755972No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT9330.1165435651838278No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT9290.11604391431487247No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG9260.115669176163156No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG9040.11292109638390176No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG8980.11217162008046878No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT8960.11192179464599111No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC8490.10605089693576614No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC8420.10517650791509432No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT8380.10467685704613902No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG8380.10467685704613902No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCT8180.10217860270136242No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC8150.10180386454964595No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG252.8954673E-431.9977515
TCAATAG400.00448251519.9998443
ACGATTA507.193707E-419.1998532
ATCGTTT2550.018.82338129
TAGATAG601.1377022E-418.6665219
CTATACT601.13824746E-418.6653564
AACTTAC601.13824746E-418.6653567
GATATAC2100.018.3038651
CGGCATC2600.017.844925
ACATCGC450.00885476117.7754176
TCTAGCG3350.017.670428
CATCGTT2850.017.40228528
GTCCTAC1950.017.2478731
GCATTAC652.1005275E-417.2295597
CTAGCGG3450.017.15928629
TGTAGGA2800.017.1480792
AACCCGT1505.456968E-1217.06653429
TCTAAAC753.2319265E-517.0665343
TAACCCG1505.456968E-1217.06546828
GTTCAAA4000.016.8166751
Produced by FastQC (version 0.11.5)