##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062950_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	800559
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22676654687537	32.0	32.0	32.0	32.0	32.0
2	30.873786941374714	32.0	32.0	32.0	32.0	32.0
3	30.93223235264359	32.0	32.0	32.0	32.0	32.0
4	30.992750065891457	32.0	32.0	32.0	32.0	32.0
5	30.935266482545323	32.0	32.0	32.0	32.0	32.0
6	34.585154873032465	36.0	36.0	36.0	32.0	36.0
7	34.49225353784043	36.0	36.0	36.0	32.0	36.0
8	34.48076281698163	36.0	36.0	36.0	32.0	36.0
9	34.58250922168135	36.0	36.0	36.0	32.0	36.0
10	34.328381793221986	36.0	36.0	36.0	32.0	36.0
11	34.573370607288155	36.0	36.0	36.0	32.0	36.0
12	34.3962081495555	36.0	36.0	36.0	32.0	36.0
13	34.49590473656532	36.0	36.0	36.0	32.0	36.0
14	34.39914984404647	36.0	36.0	36.0	32.0	36.0
15	34.34121032928241	36.0	36.0	36.0	32.0	36.0
16	34.34147264598862	36.0	36.0	36.0	32.0	36.0
17	34.26486492563321	36.0	36.0	36.0	32.0	36.0
18	34.30505809065915	36.0	36.0	36.0	32.0	36.0
19	34.28195048709714	36.0	36.0	36.0	32.0	36.0
20	34.26024815160407	36.0	36.0	36.0	32.0	36.0
21	34.23114973412328	36.0	36.0	36.0	32.0	36.0
22	34.20036749321412	36.0	36.0	36.0	32.0	36.0
23	34.14370458642024	36.0	36.0	36.0	32.0	36.0
24	34.12922095685639	36.0	36.0	36.0	32.0	36.0
25	34.113293336281274	36.0	36.0	36.0	32.0	36.0
26	34.04990887617277	36.0	36.0	36.0	32.0	36.0
27	34.0508757006042	36.0	36.0	36.0	32.0	36.0
28	34.03856680144749	36.0	36.0	36.0	32.0	36.0
29	33.995258313253615	36.0	36.0	36.0	32.0	36.0
30	33.95526875595677	36.0	36.0	36.0	32.0	36.0
31	33.98727389236771	36.0	36.0	36.0	32.0	36.0
32	33.92099020809209	36.0	36.0	36.0	32.0	36.0
33	33.88235345552295	36.0	36.0	36.0	32.0	36.0
34	33.89229276043365	36.0	36.0	36.0	32.0	36.0
35	33.81912138892949	36.0	36.0	36.0	32.0	36.0
36	33.78356123658594	36.0	36.0	36.0	32.0	36.0
37	33.77739054835434	36.0	36.0	36.0	32.0	36.0
38	33.14928818488082	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	1.0
9	2.0
10	6.0
11	5.0
12	4.0
13	7.0
14	181.0
15	304.0
16	358.0
17	447.0
18	545.0
19	672.0
20	869.0
21	1188.0
22	1731.0
23	2516.0
24	3686.0
25	5279.0
26	7769.0
27	10892.0
28	15461.0
29	20842.0
30	28096.0
31	37970.0
32	51174.0
33	76312.0
34	168870.0
35	365370.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.78011448123891	17.245221715167578	11.455002269865071	25.519661533728446
2	16.715554616818036	20.186369947845908	37.03002890783861	26.06804652749744
3	18.75246728196723	24.70942289337844	28.540268540218573	27.997841284435758
4	12.28828432701304	15.375314005618687	36.15183694067652	36.18456472669176
5	14.618376774083947	36.66873190315707	33.0930830399948	15.619808282764186
6	33.89473342343715	35.768926173993634	16.894715935634892	13.441624466934313
7	29.820075497340603	30.64125272622346	21.22906772526163	18.309604051174304
8	28.447193641427155	32.1764721312786	19.847306312979587	19.52902791431466
9	27.82182360987065	14.049503157184704	18.593101036156167	39.53557219678848
10	16.494307074467088	25.863931897246285	30.689584101029908	26.952176927256716
11	38.149492597570664	20.930037924022045	21.955835252851795	18.964634225555493
12	25.212794395768846	23.867324224459054	28.19341492846061	22.72646645131149
13	29.560261501924533	18.690666410145255	25.57476828745723	26.17430380047298
14	24.20460621324099	20.26598907660865	24.435091769479463	31.094312940670893
15	25.638446737799548	26.79027461955571	22.600139933531572	24.971138709113173
16	26.18326914211972	25.274931196929817	23.898245162547642	24.64355449840282
17	24.003288327063608	25.533135017559815	25.421816650778915	25.041760004597663
18	25.628780364713847	23.72139039971618	26.729067928516287	23.920761307053684
19	25.955018563212363	24.857840271427644	25.15115401719908	24.035987148160917
20	25.832398521957035	23.621829865162308	25.157710382527316	25.388061230353347
21	27.92965992971989	23.74483298792896	23.670380544427246	24.655126537923902
22	26.004297260496433	24.136238148180535	24.68638742801464	25.17307716330839
23	24.065294762102855	23.60320452521134	25.537387584900806	26.794113127785003
24	24.976888173740967	25.387154322036437	24.712538852853875	24.92341865136872
25	25.169909947727703	24.100726614894104	24.97176496456878	25.757598472809413
26	24.412247539422953	25.441204764486734	25.82700125683702	24.319546439253294
27	25.733077658198987	24.452869786819406	24.70746225772803	25.10659029725357
28	24.30760544097017	24.16469815207384	25.883707944471023	25.643988462484963
29	24.113712625269528	24.582185055354703	25.934508803533905	25.369593515841864
30	24.329358094983505	24.919585429317	26.250188935204903	24.500867540494593
31	25.140596463789695	24.85690515391284	24.28415101221959	25.718347370077876
32	24.33027321230261	24.92676571148751	24.33676904684871	26.40619202936117
33	24.048249904122304	24.23850623173795	25.404903691322538	26.308340172817207
34	25.161812600957195	24.667848482690644	25.71736607438445	24.45297284196771
35	26.425682842333163	24.24126200432012	25.429731860312003	23.903323293034713
36	24.443936395618454	25.54619413128648	24.614715759174864	25.395153713920205
37	25.7152208593019	25.333453260327776	24.620262415254714	24.33106346511561
38	24.51059630362954	24.846505062366102	25.210021048949738	25.43287758505462
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	429.0
1	346.0
2	263.0
3	263.0
4	538.0
5	813.0
6	813.0
7	933.0
8	1053.0
9	1031.5
10	1010.0
11	1010.0
12	1229.5
13	1449.0
14	1738.5
15	2028.0
16	2028.0
17	2959.0
18	3890.0
19	3890.0
20	4425.5
21	4961.0
22	5197.0
23	5433.0
24	5433.0
25	6535.5
26	7638.0
27	7638.0
28	10024.5
29	12411.0
30	15258.5
31	18106.0
32	18106.0
33	23599.5
34	29093.0
35	29093.0
36	31440.0
37	33787.0
38	38754.5
39	43722.0
40	43722.0
41	45302.5
42	46883.0
43	53833.0
44	60783.0
45	60783.0
46	63804.0
47	66825.0
48	66825.0
49	69933.5
50	73042.0
51	73054.5
52	73067.0
53	73067.0
54	68352.5
55	63638.0
56	63638.0
57	63181.5
58	62725.0
59	56321.0
60	49917.0
61	49917.0
62	49301.0
63	48685.0
64	39851.0
65	31017.0
66	31017.0
67	26191.0
68	21365.0
69	21365.0
70	17369.5
71	13374.0
72	10461.0
73	7548.0
74	7548.0
75	5638.0
76	3728.0
77	3728.0
78	3949.0
79	4170.0
80	3239.0
81	2308.0
82	2308.0
83	2557.5
84	2807.0
85	2807.0
86	1832.5
87	858.0
88	777.0
89	696.0
90	696.0
91	483.0
92	270.0
93	244.5
94	219.0
95	219.0
96	185.0
97	151.0
98	151.0
99	274.0
100	397.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11891690681136557
2	0.053712468412696626
3	0.010367755530822836
4	0.00199860347582127
5	6.245635861941468E-4
6	1.2491271723882936E-4
7	1.2491271723882936E-4
8	2.498254344776587E-4
9	4.996508689553175E-4
10	4.996508689553175E-4
11	0.0013740398896271231
12	0.0026231670620154167
13	0.013240748027315914
14	0.009368453792912203
15	0.022359376385750456
16	0.010742493682539326
17	0.018986733020302064
18	0.005121421406792004
19	0.006370548579180297
20	0.005121421406792004
21	0.00599581042746381
22	0.006120723144702639
23	0.008244239337762738
24	0.012866009875599424
25	0.016863216827241966
26	0.017113042261719624
27	0.00799441390328508
28	0.004996508689553174
29	0.010617580965300497
30	0.002498254344776587
31	0.005121421406792004
32	0.005745984992986151
33	0.007494763034329762
34	0.01199162085492762
35	0.014739700634181866
36	0.013865311613510059
37	0.008993715641195714
38	0.0054961595585084925
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	800559.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.227944300270146
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.74193260010478	40.239873050343945
2	11.439581758287739	11.262941872307504
3	3.097052201412524	4.573845397984943
4	1.1835665048335209	2.3305818390243993
5	0.6006342635441881	1.4783995035293536
6	0.34835916550295876	1.0289403357520945
7	0.23306889593460478	0.8031451839035929
8	0.1748403141381603	0.6885623396668072
9	0.1354260175611389	0.6000070004376438
>10	0.7454770586527142	7.266966623621494
>50	0.12939829597870595	4.491609826102768
>100	0.14773805365433768	15.498799662636381
>500	0.01732101318699751	5.8158900587492495
>1k	0.005603857207558018	3.9204373059398305
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2412	0.30128947398005645	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1977	0.24695244198116567	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1778	0.2220948112506386	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1672	0.2088540632233227	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1572	0.19636279149943978	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1536	0.19186593367884192	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1527	0.19074171922369246	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1519	0.1897424174857818	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1458	0.18212274173421322	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1426	0.17812553478257068	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1371	0.17125533533443507	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1344	0.16788269196898667	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1318	0.1646349613207771	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1310	0.16363565958286647	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1230	0.15364264220376012	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1208	0.15089456242450586	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1185	0.1480215699280128	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1121	0.1400271560247277	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1114	0.1391527670040559	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1111	0.13877802885233942	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	1052	0.1314081785352485	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1024	0.12791062245256127	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	981	0.12253937561129162	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	976	0.12191481202509748	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	973	0.12154007387338098	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	961	0.12004112126651502	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	957	0.11954147039755972	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	933	0.1165435651838278	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT	929	0.11604391431487247	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	926	0.115669176163156	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	904	0.11292109638390176	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	898	0.11217162008046878	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	896	0.11192179464599111	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	849	0.10605089693576614	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	842	0.10517650791509432	No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT	838	0.10467685704613902	No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG	838	0.10467685704613902	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCT	818	0.10217860270136242	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	815	0.10180386454964595	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	2.498254344776587E-4
16	0.0	0.0	0.0	0.0	2.498254344776587E-4
17	0.0	0.0	0.0	1.2491271723882936E-4	2.498254344776587E-4
18	0.0	0.0	0.0	1.2491271723882936E-4	3.747381517164881E-4
19	0.0	0.0	0.0	1.2491271723882936E-4	3.747381517164881E-4
20	0.0	0.0	0.0	2.498254344776587E-4	6.245635861941468E-4
21	0.0	0.0	0.0	4.996508689553175E-4	6.245635861941468E-4
22	0.0	0.0	0.0	8.743890206718056E-4	6.245635861941468E-4
23	0.0	0.0	0.0	0.002373341627537758	6.245635861941468E-4
24	0.0	0.0	0.0	0.003622468799926052	6.245635861941468E-4
25	0.0	0.0	0.0	0.004496857820597857	6.245635861941468E-4
26	0.0	0.0	0.0	0.00587089771022498	6.245635861941468E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	25	2.8954673E-4	31.997751	5
TCAATAG	40	0.004482515	19.999844	3
ACGATTA	50	7.193707E-4	19.19985	32
ATCGTTT	255	0.0	18.823381	29
TAGATAG	60	1.1377022E-4	18.666521	9
CTATACT	60	1.13824746E-4	18.665356	4
AACTTAC	60	1.13824746E-4	18.665356	7
GATATAC	210	0.0	18.303865	1
CGGCATC	260	0.0	17.8449	25
ACATCGC	45	0.008854761	17.775417	6
TCTAGCG	335	0.0	17.6704	28
CATCGTT	285	0.0	17.402285	28
GTCCTAC	195	0.0	17.247873	1
GCATTAC	65	2.1005275E-4	17.229559	7
CTAGCGG	345	0.0	17.159286	29
TGTAGGA	280	0.0	17.148079	2
AACCCGT	150	5.456968E-12	17.066534	29
TCTAAAC	75	3.2319265E-5	17.066534	3
TAACCCG	150	5.456968E-12	17.065468	28
GTTCAAA	400	0.0	16.816675	1
>>END_MODULE