FastQCFastQC Report
Thu 2 Feb 2017
SRR4062950_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062950_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences800559
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT31830.3975971789711939No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT22580.2820529155252767No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA20310.2536977287120624No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC19550.2442043622019114No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG16720.2088540632233227No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA16420.20510668170615784No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC15570.19448910074085735No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC15380.19211575911331957No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT15330.19149119552712543No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC14880.18587012325137808No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA13800.17237954978958453No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG13770.17200481163786804No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT13750.1717549862033904No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG13540.16913181914137496No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG12880.16088757980361224No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA12830.1602630162174181No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT12650.15801458730711915No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC12640.15788967458988032No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG11470.14327488667293728No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG11280.14090154504539953No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG10860.1356552109213687No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA10670.13328186929383093No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT10460.13065870223181553No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT10360.1294095750594272No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG10170.12703623343188947No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC10070.12578710625950118No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10030.12528745539054587No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT9860.12316393919748576No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC9570.11954147039755972No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT9570.11954147039755972No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT9440.11791760507345493No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG9400.11741795420449962No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT9210.11504461257696186No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG8710.10879897671502037No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT8570.10705019867367678No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG8520.10642563508748261No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC8370.10455194432890018No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCT8310.1038024680254672No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAC308.415962E-426.665941
AAACGGG350.00206632422.85651829
TCCTCGC502.7968295E-522.39938729
GTCCTAA1250.020.479441
GTAATAC400.004479781720.0019513
TAACTTT701.7846565E-518.2874986
TCTAGAC450.0088430217.7795123
TAACACC450.0088430217.7795124
CGGCTAA450.0088430217.7795129
TAGTAGT450.0088430217.7795124
AACGGGA450.00884938617.77729230
CTAAACG450.00884938617.77729212
ATAATAC550.001363263517.456253
GCGACGC550.001364405317.4540732
TAGGGGT753.229006E-517.0683334
TCCTACA2350.017.0208112
TGGATAC3600.016.88842824
TAACAGT1051.18641765E-716.7635424
AGGCCCG3750.016.63954510
GTCCTAC1750.016.4566921
Produced by FastQC (version 0.11.5)