##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062950_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	800559
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.204312236824517	32.0	32.0	32.0	32.0	32.0
2	31.330000662037403	32.0	32.0	32.0	32.0	32.0
3	31.39250074010785	32.0	32.0	32.0	32.0	32.0
4	31.50011804251779	32.0	32.0	32.0	32.0	32.0
5	31.422053839879386	32.0	32.0	32.0	32.0	32.0
6	34.94634374231006	36.0	36.0	36.0	36.0	36.0
7	34.97198957228637	36.0	36.0	36.0	36.0	36.0
8	34.9143623393154	36.0	36.0	36.0	36.0	36.0
9	35.00638553810525	36.0	36.0	36.0	36.0	36.0
10	34.86559391625102	36.0	36.0	36.0	32.0	36.0
11	35.03293698528154	36.0	36.0	36.0	36.0	36.0
12	34.91400758719845	36.0	36.0	36.0	32.0	36.0
13	34.985196593880026	36.0	36.0	36.0	36.0	36.0
14	34.91632221984888	36.0	36.0	36.0	32.0	36.0
15	34.891224756701256	36.0	36.0	36.0	32.0	36.0
16	34.89517074943883	36.0	36.0	36.0	32.0	36.0
17	34.84713181664312	36.0	36.0	36.0	32.0	36.0
18	34.86074480456781	36.0	36.0	36.0	32.0	36.0
19	34.849597593681416	36.0	36.0	36.0	32.0	36.0
20	34.83078823671959	36.0	36.0	36.0	32.0	36.0
21	34.81599357448982	36.0	36.0	36.0	32.0	36.0
22	34.79984236015085	36.0	36.0	36.0	32.0	36.0
23	34.72782768040832	36.0	36.0	36.0	32.0	36.0
24	34.70034063697991	36.0	36.0	36.0	32.0	36.0
25	34.66904125742138	36.0	36.0	36.0	32.0	36.0
26	34.61559735135074	36.0	36.0	36.0	32.0	36.0
27	34.59584615250094	36.0	36.0	36.0	32.0	36.0
28	34.553135996222636	36.0	36.0	36.0	32.0	36.0
29	34.50965263022463	36.0	36.0	36.0	32.0	36.0
30	34.49344770341724	36.0	36.0	36.0	32.0	36.0
31	34.48496113340803	36.0	36.0	36.0	32.0	36.0
32	34.44414590305024	36.0	36.0	36.0	32.0	36.0
33	34.39650794007687	36.0	36.0	36.0	32.0	36.0
34	34.39090060820002	36.0	36.0	36.0	32.0	36.0
35	34.33468863631538	36.0	36.0	36.0	32.0	36.0
36	34.31101267988993	36.0	36.0	36.0	32.0	36.0
37	34.278621563182725	36.0	36.0	36.0	32.0	36.0
38	33.83121793646689	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	3.0
21	23.0
22	51.0
23	207.0
24	534.0
25	1342.0
26	2903.0
27	5350.0
28	9815.0
29	15297.0
30	22858.0
31	32998.0
32	47165.0
33	74813.0
34	179832.0
35	407365.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.33367062547705	18.516339305902736	12.038706147336704	26.11128392128351
2	15.73587955416153	21.136855622134036	36.41505498033249	26.71220984337194
3	17.919728589647985	25.24323628864331	28.91829334252691	27.9187417791818
4	11.819990781441469	16.085385336995774	36.09565316235281	35.99897071920995
5	13.988992677607367	37.05565368155712	33.028462647303506	15.92689099353201
6	33.91117956328016	36.20370266276439	16.575293014006462	13.309824759948985
7	29.581366996519925	30.860224993067337	21.12514021470025	18.433267795712492
8	28.34492131580854	32.42415763320351	19.580983883418984	19.64993716756897
9	27.75237781158888	13.762451188343663	18.590901482275406	39.894269517792054
10	16.241438709089113	26.0864079864993	30.670375778682434	27.001777525729153
11	38.23367382502882	20.81263296227775	21.922551776432243	19.03114143626118
12	24.994066608873354	24.05509184889464	28.159749373558814	22.791092168673195
13	29.716522725399034	18.692836746369405	25.504835377324316	26.085805150907245
14	24.160867593768852	20.15166902127139	24.340866819310005	31.346596565649754
15	25.542027508278593	26.845241887231296	22.601082493607592	25.011648110882522
16	26.231828299760917	25.24689029401992	23.80577047509362	24.71551093112554
17	24.07167991366033	25.60111122353256	25.17116165079651	25.156047212010606
18	25.731520100329895	23.527060466499034	26.777164456336134	23.964254976834937
19	25.857432119306633	24.804542825700544	25.23586643832622	24.102158616666607
20	25.81970847870051	23.474722037975965	25.338794517331014	25.366774965992512
21	27.84554292687984	23.740286474825716	23.76764235990102	24.646528238393422
22	25.958986158421805	24.077300985936077	24.80966424710733	25.154048608534787
23	23.86682305738865	23.37041991908154	25.71678039969571	27.045976623834093
24	24.85900477041667	25.1209467384665	25.0225155172823	24.99753297383453
25	25.209041693418836	23.811391554390813	25.166571316506737	25.81299543568361
26	24.473524125017644	25.249357011788014	25.947244363001353	24.32987450019299
27	25.822585581253005	24.076047242225705	24.951439938542634	25.149927237978652
28	24.428680459528902	23.870820264340292	26.135487827880265	25.565011448250534
29	24.25854933864962	24.194094376554382	26.11462740410138	25.432728880694615
30	24.38958277903315	24.47077604523839	26.49498662809362	24.64465454763484
31	25.14630402006598	24.315259712276045	24.549221231664376	25.989215035993602
32	24.51324636909959	24.558964423609	24.685376093454696	26.242413113836704
33	24.11102741959056	23.7952480704108	25.75125630965363	26.34246820034501
34	25.21338214922323	24.223823603257223	26.03880538473741	24.523988862782133
35	26.553320867044157	23.973248692476133	25.636711347945624	23.836719092534093
36	24.537104698092207	25.12919097780426	24.948442275959675	25.385262048143858
37	25.884413266230222	24.97142621593162	24.905971952098472	24.23818856573969
38	24.73649629383505	24.305297055303924	25.429013263248883	25.529193387612143
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	187.0
1	165.5
2	144.0
3	144.0
4	471.5
5	799.0
6	799.0
7	965.5
8	1132.0
9	1085.5
10	1039.0
11	1039.0
12	1269.5
13	1500.0
14	1852.0
15	2204.0
16	2204.0
17	3299.5
18	4395.0
19	4395.0
20	5009.5
21	5624.0
22	5566.0
23	5508.0
24	5508.0
25	6377.5
26	7247.0
27	7247.0
28	9594.5
29	11942.0
30	14805.5
31	17669.0
32	17669.0
33	23134.0
34	28599.0
35	28599.0
36	31259.0
37	33919.0
38	38948.5
39	43978.0
40	43978.0
41	44918.5
42	45859.0
43	53300.5
44	60742.0
45	60742.0
46	63821.5
47	66901.0
48	66901.0
49	70497.0
50	74093.0
51	73407.0
52	72721.0
53	72721.0
54	68122.5
55	63524.0
56	63524.0
57	63497.0
58	63470.0
59	56627.5
60	49785.0
61	49785.0
62	49251.0
63	48717.0
64	40307.5
65	31898.0
66	31898.0
67	26844.5
68	21791.0
69	21791.0
70	17773.0
71	13755.0
72	10847.5
73	7940.0
74	7940.0
75	5836.0
76	3732.0
77	3732.0
78	3906.5
79	4081.0
80	3147.0
81	2213.0
82	2213.0
83	2290.5
84	2368.0
85	2368.0
86	1460.5
87	553.0
88	482.5
89	412.0
90	412.0
91	242.0
92	72.0
93	50.5
94	29.0
95	29.0
96	19.0
97	9.0
98	9.0
99	8.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008244239337762738
2	0.0
3	0.0
4	0.0
5	1.2491271723882936E-4
6	0.0
7	1.2491271723882936E-4
8	0.0021235161930600994
9	0.004122119668881369
10	9.99301737910635E-4
11	0.006245635861941468
12	6.245635861941468E-4
13	1.2491271723882936E-4
14	0.0
15	0.0
16	1.2491271723882936E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	1.2491271723882936E-4
26	0.0
27	4.996508689553175E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.2491271723882936E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	800559.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.9292081275653
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.47145866378675	37.76461831961616
2	12.171352919387852	11.423839086960042
3	3.2894520316589757	4.631141370580998
4	1.3375940490212153	2.5108891806683764
5	0.6476647262208729	1.5197196366850974
6	0.3889491349198506	1.0951844942214088
7	0.24931332366552364	0.819005379869209
8	0.18358600712796447	0.6892436750253546
9	0.136341002887619	0.5758537770750649
>10	0.8022058303396329	7.486780891078701
>50	0.13558544965457842	4.530874603682773
>100	0.15862951423411026	15.674019242810115
>500	0.020632555060830962	6.377123333147412
>1k	0.0072347920343173505	4.901707008579381
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3183	0.3975971789711939	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2258	0.2820529155252767	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2031	0.2536977287120624	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1955	0.2442043622019114	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1672	0.2088540632233227	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1642	0.20510668170615784	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1557	0.19448910074085735	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1538	0.19211575911331957	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1533	0.19149119552712543	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1488	0.18587012325137808	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1380	0.17237954978958453	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1377	0.17200481163786804	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1375	0.1717549862033904	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1354	0.16913181914137496	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1288	0.16088757980361224	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1283	0.1602630162174181	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1265	0.15801458730711915	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1264	0.15788967458988032	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1147	0.14327488667293728	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1128	0.14090154504539953	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1086	0.1356552109213687	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1067	0.13328186929383093	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1046	0.13065870223181553	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1036	0.1294095750594272	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	1017	0.12703623343188947	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	1007	0.12578710625950118	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1003	0.12528745539054587	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	986	0.12316393919748576	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	957	0.11954147039755972	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	957	0.11954147039755972	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT	944	0.11791760507345493	No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG	940	0.11741795420449962	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	921	0.11504461257696186	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	871	0.10879897671502037	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	857	0.10705019867367678	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	852	0.10642563508748261	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	837	0.10455194432890018	No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCT	831	0.1038024680254672	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	1.2491271723882936E-4
16	0.0	0.0	0.0	0.0	1.2491271723882936E-4
17	0.0	0.0	0.0	0.0	1.2491271723882936E-4
18	0.0	0.0	0.0	0.0	2.498254344776587E-4
19	0.0	0.0	0.0	0.0	2.498254344776587E-4
20	0.0	0.0	0.0	1.2491271723882936E-4	2.498254344776587E-4
21	0.0	0.0	0.0	2.498254344776587E-4	2.498254344776587E-4
22	0.0	0.0	0.0	6.245635861941468E-4	2.498254344776587E-4
23	0.0	0.0	0.0	0.0021235161930600994	2.498254344776587E-4
24	0.0	0.0	0.0	0.003622468799926052	2.498254344776587E-4
25	0.0	0.0	0.0	0.004496857820597857	2.498254344776587E-4
26	0.0	0.0	0.0	0.00587089771022498	3.747381517164881E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAC	30	8.415962E-4	26.66594	1
AAACGGG	35	0.002066324	22.856518	29
TCCTCGC	50	2.7968295E-5	22.399387	29
GTCCTAA	125	0.0	20.47944	1
GTAATAC	40	0.0044797817	20.001951	3
TAACTTT	70	1.7846565E-5	18.287498	6
TCTAGAC	45	0.00884302	17.779512	3
TAACACC	45	0.00884302	17.779512	4
CGGCTAA	45	0.00884302	17.779512	9
TAGTAGT	45	0.00884302	17.779512	4
AACGGGA	45	0.008849386	17.777292	30
CTAAACG	45	0.008849386	17.777292	12
ATAATAC	55	0.0013632635	17.45625	3
GCGACGC	55	0.0013644053	17.45407	32
TAGGGGT	75	3.229006E-5	17.068333	4
TCCTACA	235	0.0	17.020811	2
TGGATAC	360	0.0	16.888428	24
TAACAGT	105	1.18641765E-7	16.763542	4
AGGCCCG	375	0.0	16.639545	10
GTCCTAC	175	0.0	16.456692	1
>>END_MODULE