FastQCFastQC Report
Thu 2 Feb 2017
SRR4062949_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062949_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1470146
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT25940.1764450605586112No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC25190.17134352642526662No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT25080.17059530141904272No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT22130.15052926716122073No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA21240.14447544665631848No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC20860.14189066936209058No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG20180.13726527841452482No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC19900.1353607056714095No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG19170.13039521244828745No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG19000.129238864711396No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC18590.1264500260518343No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG17960.12216473737982486No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG16910.11502258959314245No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC16600.11291395548469335No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG16580.11277791457447084No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA16390.11148552592735686No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC16390.11148552592735686No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA16090.10944491227401903No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT15590.10604388951845599No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG15250.10373119404467311No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT14860.10107839629533394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATTCC4950.019.3916914
CTAGCGG3750.019.19973429
ATCGTTT4150.018.89130629
CGTTTAT4250.018.82326931
AAGACGG5950.018.550585
TCTAGCG3800.018.52605828
CATCGTT4250.018.44680428
CGGCATC4200.018.2854625
CCTATTC5350.017.9424573
GTATCAA25450.017.8738271
CGCAAGA5400.017.7823722
GGATCGT450.00886253417.7739036
TCGTTTA4450.017.61773330
GTATTAT1101.0497388E-817.4733261
GTCCTAC4250.017.3362791
GTCCTAT5800.017.1218511
CAAGACG6200.017.0302854
TCCTATA957.6752076E-716.8464572
GGCATCG4700.016.68005226
GCGCAAG6050.016.6790831
Produced by FastQC (version 0.11.5)