##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062949_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1470146
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.260068047663292	32.0	32.0	32.0	32.0	32.0
2	30.840955932267953	32.0	32.0	32.0	32.0	32.0
3	30.88484545072394	32.0	32.0	32.0	32.0	32.0
4	30.961639184135453	32.0	32.0	32.0	32.0	32.0
5	30.856268697122598	32.0	32.0	32.0	32.0	32.0
6	34.529690928656066	36.0	36.0	36.0	32.0	36.0
7	34.45433854868836	36.0	36.0	36.0	32.0	36.0
8	34.43715590152271	36.0	36.0	36.0	32.0	36.0
9	34.59655775684864	36.0	36.0	36.0	32.0	36.0
10	34.28370855683721	36.0	36.0	36.0	32.0	36.0
11	34.57879149417813	36.0	36.0	36.0	32.0	36.0
12	34.36485083794398	36.0	36.0	36.0	32.0	36.0
13	34.47407400353434	36.0	36.0	36.0	32.0	36.0
14	34.36167904412215	36.0	36.0	36.0	32.0	36.0
15	34.290925527124514	36.0	36.0	36.0	32.0	36.0
16	34.31825206476091	36.0	36.0	36.0	32.0	36.0
17	34.22637003399662	36.0	36.0	36.0	32.0	36.0
18	34.25558686008056	36.0	36.0	36.0	32.0	36.0
19	34.24773457874252	36.0	36.0	36.0	32.0	36.0
20	34.234141370993086	36.0	36.0	36.0	32.0	36.0
21	34.21821846265609	36.0	36.0	36.0	32.0	36.0
22	34.19292709703662	36.0	36.0	36.0	32.0	36.0
23	34.13128151897839	36.0	36.0	36.0	32.0	36.0
24	34.108426646061005	36.0	36.0	36.0	32.0	36.0
25	34.08869799326053	36.0	36.0	36.0	32.0	36.0
26	34.03437345678592	36.0	36.0	36.0	32.0	36.0
27	34.04463570284856	36.0	36.0	36.0	32.0	36.0
28	34.022340638276745	36.0	36.0	36.0	32.0	36.0
29	33.98344858265778	36.0	36.0	36.0	32.0	36.0
30	33.95779874923987	36.0	36.0	36.0	32.0	36.0
31	33.973024447911975	36.0	36.0	36.0	32.0	36.0
32	33.9344316822955	36.0	36.0	36.0	32.0	36.0
33	33.90420815347591	36.0	36.0	36.0	32.0	36.0
34	33.907437084480044	36.0	36.0	36.0	32.0	36.0
35	33.89176653203151	36.0	36.0	36.0	32.0	36.0
36	33.82895440316812	36.0	36.0	36.0	32.0	36.0
37	33.83629585088828	36.0	36.0	36.0	32.0	36.0
38	33.22926566477071	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	4.0
10	15.0
11	19.0
12	5.0
13	12.0
14	97.0
15	287.0
16	352.0
17	478.0
18	543.0
19	759.0
20	1259.0
21	1749.0
22	2716.0
23	4262.0
24	6428.0
25	10095.0
26	14626.0
27	21162.0
28	29585.0
29	39773.0
30	53178.0
31	71843.0
32	98030.0
33	146017.0
34	320440.0
35	646409.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.301532753357876	16.768891086384745	11.17869318917034	25.75088297108704
2	17.131882446299528	18.880610118460222	37.42073047066942	26.566776964570828
3	19.5867367729304	22.81743912585317	27.865675165089637	29.73014893612679
4	12.562963446679444	14.574936789971288	35.528967777000055	37.333131986349215
5	15.018093514903342	36.26470948345056	33.15044825662862	15.566748745017481
6	35.66481038213342	34.84284840705755	16.273949233440874	13.218391977368157
7	31.407832963528794	29.796156300122572	20.01685546877657	18.779155267572065
8	28.8859655149193	32.73892403664133	18.70049376149123	19.67461668694814
9	27.581447803752557	13.968359562056506	18.171893365547863	40.27829926864307
10	16.27398404510034	25.84515530451439	30.84371326890427	27.037147381481
11	38.736451730954194	20.63220947491147	21.53751341732682	19.093825376807512
12	25.501714498720844	23.122979316499663	27.95779088489357	23.417515299885928
13	29.83522198872453	18.673775458070082	25.00120755117466	26.489795002030725
14	23.85654372974058	19.59843400827894	24.191782965248184	32.3532392967323
15	25.54085692066207	27.146775202058038	21.645571256627825	25.66679662065207
16	26.49831391002123	25.260532705111466	23.406275914662146	24.834877470205154
17	24.513641973544736	25.787487022061473	24.489625133181203	25.209245871212588
18	25.61807001074786	24.14642939743956	25.83200685686299	24.403493734949596
19	26.095356266292253	24.468444052614107	24.702861994089893	24.73333768700375
20	26.366370942177948	23.744916881056717	24.306525847209166	25.582186329556166
21	27.54212441753682	23.746471208462296	23.77647018808884	24.934934185912045
22	26.547735258004064	23.909430727839815	24.514178233851574	25.028655780304547
23	24.575493486058853	23.72733079773415	25.324948758538255	26.372226957668737
24	25.35872798173501	24.530313296814242	24.852848734213385	25.258109987237365
25	25.224475633899594	24.202956724064034	24.834713273963	25.737854368073364
26	24.817139338769316	25.368085533410213	25.415505822082068	24.3992693057384
27	25.776745888296077	24.242199548166646	24.48825212872557	25.49280243481171
28	24.794105782724547	24.361671805944145	25.285313230629413	25.558909180701896
29	24.720061933321	24.58747401303974	25.248440782384378	25.444023271254885
30	24.603529832705032	24.869226405314475	25.611625891607225	24.915617870373268
31	25.66152196819579	24.68448502336984	24.017095935735824	25.636897072698538
32	25.323426133402993	24.490524760517047	23.89469144419003	26.29135766188993
33	24.568727717003476	24.133560905326558	25.08906395938534	26.208647418284624
34	25.781487039730493	24.46852078364357	24.969556870487104	24.78043530613883
35	26.19404787416788	23.974229451763563	25.383717884610807	24.44800478945775
36	24.48396184504943	25.01134430141894	24.791533579472357	25.71316027405927
37	26.020017700692318	24.697023603417417	24.403213879737333	24.879744816152936
38	25.006003209421195	24.366159721423873	24.91573398673648	25.71210308241845
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	37.0
1	69.5
2	102.0
3	102.0
4	234.0
5	366.0
6	366.0
7	493.0
8	620.0
9	638.0
10	656.0
11	656.0
12	901.0
13	1146.0
14	1611.5
15	2077.0
16	2077.0
17	3071.5
18	4066.0
19	4066.0
20	5144.5
21	6223.0
22	6963.0
23	7703.0
24	7703.0
25	9836.5
26	11970.0
27	11970.0
28	15735.0
29	19500.0
30	24584.5
31	29669.0
32	29669.0
33	39043.5
34	48418.0
35	48418.0
36	54580.5
37	60743.0
38	69733.0
39	78723.0
40	78723.0
41	82890.0
42	87057.0
43	98974.0
44	110891.0
45	110891.0
46	114239.5
47	117588.0
48	117588.0
49	126641.0
50	135694.0
51	138900.0
52	142106.0
53	142106.0
54	135911.5
55	129717.0
56	129717.0
57	126308.0
58	122899.0
59	111453.5
60	100008.0
61	100008.0
62	94716.5
63	89425.0
64	75076.5
65	60728.0
66	60728.0
67	50542.0
68	40356.0
69	40356.0
70	32324.5
71	24293.0
72	18740.5
73	13188.0
74	13188.0
75	9903.0
76	6618.0
77	6618.0
78	6397.0
79	6176.0
80	4869.5
81	3563.0
82	3563.0
83	3613.0
84	3663.0
85	3663.0
86	2515.5
87	1368.0
88	1170.5
89	973.0
90	973.0
91	703.0
92	433.0
93	393.5
94	354.0
95	354.0
96	307.5
97	261.0
98	261.0
99	514.5
100	768.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12298098284115999
2	0.05387220044811876
3	0.012719825105805818
4	0.0031969613902292694
5	4.0812273066756635E-4
6	3.401022755563053E-4
7	0.0
8	2.0406136533378317E-4
9	1.3604091022252212E-4
10	1.3604091022252212E-4
11	0.002176654563560354
12	0.002516756839116659
13	0.015576684220478781
14	0.01027108872180042
15	0.026868079768948117
16	0.012039620554693207
17	0.023399036558273804
18	0.005849759139568451
19	0.008298495523573849
20	0.005713718229345929
21	0.0065299636906810616
22	0.0075502705173499775
23	0.009862965991132854
24	0.015644704675590045
25	0.018773645610708052
26	0.02115436153960219
27	0.009182761440020244
28	0.006461943235569801
29	0.012107641009804469
30	0.0032649818453405308
31	0.006053820504902234
32	0.00707412733157115
33	0.009182761440020244
34	0.013059927381362123
35	0.017073134232926526
36	0.016665011502258957
37	0.009999006901355375
38	0.006734025056014845
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1470146.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.7753270982577
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.93311005853253	41.65720702221829
2	12.79913540501253	13.509571151488545
3	3.7326486005256503	5.909752525067851
4	1.5660741086535817	3.3060029337722048
5	0.8150354591908446	2.1506881477737743
6	0.4875388532103482	1.5438013470771341
7	0.30939385903069333	1.1429853478775949
8	0.21614281887517495	0.912560637286146
9	0.1579979389533573	0.7504553618202583
>10	0.8101634044161673	7.691937530810423
>50	0.07493407348854854	2.7815385415479716
>100	0.07854506722665855	9.142529542783327
>500	0.013198630833317022	4.661024533873997
>1k	0.00608172205064608	4.83994537660252
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2594	0.1764450605586112	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2519	0.17134352642526662	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2508	0.17059530141904272	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2213	0.15052926716122073	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2124	0.14447544665631848	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2086	0.14189066936209058	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2018	0.13726527841452482	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1990	0.1353607056714095	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1917	0.13039521244828745	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1900	0.129238864711396	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1859	0.1264500260518343	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1796	0.12216473737982486	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1691	0.11502258959314245	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1660	0.11291395548469335	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1658	0.11277791457447084	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1639	0.11148552592735686	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1639	0.11148552592735686	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1609	0.10944491227401903	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1559	0.10604388951845599	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1525	0.10373119404467311	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1486	0.10107839629533394	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.0406136533378317E-4
2	0.0	0.0	0.0	0.0	2.0406136533378317E-4
3	0.0	0.0	0.0	0.0	2.0406136533378317E-4
4	0.0	0.0	0.0	0.0	2.0406136533378317E-4
5	0.0	0.0	0.0	0.0	3.401022755563053E-4
6	0.0	0.0	0.0	0.0	3.401022755563053E-4
7	0.0	0.0	0.0	0.0	3.401022755563053E-4
8	0.0	0.0	0.0	0.0	3.401022755563053E-4
9	0.0	0.0	0.0	0.0	3.401022755563053E-4
10	0.0	0.0	0.0	0.0	3.401022755563053E-4
11	0.0	0.0	0.0	0.0	3.401022755563053E-4
12	0.0	0.0	0.0	0.0	0.001020306826668916
13	0.0	0.0	0.0	0.0	0.001020306826668916
14	0.0	0.0	0.0	0.0	0.001020306826668916
15	0.0	0.0	0.0	0.0	0.001224368192002699
16	0.0	0.0	0.0	0.0	0.001224368192002699
17	0.0	0.0	0.0	1.3604091022252212E-4	0.001224368192002699
18	0.0	0.0	6.802045511126106E-5	2.0406136533378317E-4	0.0014284295573364822
19	0.0	0.0	6.802045511126106E-5	2.0406136533378317E-4	0.0014964500124477432
20	0.0	0.0	6.802045511126106E-5	2.7208182044504423E-4	0.0015644704675590044
21	0.0	0.0	6.802045511126106E-5	3.401022755563053E-4	0.0015644704675590044
22	0.0	0.0	6.802045511126106E-5	4.7614318577882743E-4	0.0016324909226702654
23	0.0	0.0	6.802045511126106E-5	6.802045511126106E-4	0.0016324909226702654
24	0.0	0.0	6.802045511126106E-5	8.162454613351327E-4	0.0016324909226702654
25	0.0	0.0	6.802045511126106E-5	0.001156347736891438	0.0016324909226702654
26	0.0	0.0	6.802045511126106E-5	0.0013604091022252213	0.0017685318328927876
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATTCC	495	0.0	19.391691	4
CTAGCGG	375	0.0	19.199734	29
ATCGTTT	415	0.0	18.891306	29
CGTTTAT	425	0.0	18.823269	31
AAGACGG	595	0.0	18.55058	5
TCTAGCG	380	0.0	18.526058	28
CATCGTT	425	0.0	18.446804	28
CGGCATC	420	0.0	18.28546	25
CCTATTC	535	0.0	17.942457	3
GTATCAA	2545	0.0	17.873827	1
CGCAAGA	540	0.0	17.782372	2
GGATCGT	45	0.008862534	17.773903	6
TCGTTTA	445	0.0	17.617733	30
GTATTAT	110	1.0497388E-8	17.473326	1
GTCCTAC	425	0.0	17.336279	1
GTCCTAT	580	0.0	17.121851	1
CAAGACG	620	0.0	17.030285	4
TCCTATA	95	7.6752076E-7	16.846457	2
GGCATCG	470	0.0	16.680052	26
GCGCAAG	605	0.0	16.679083	1
>>END_MODULE